Pigment Cell & Melanoma Research,
Journal Year:
2024,
Volume and Issue:
37(4), P. 480 - 495
Published: April 13, 2024
Abstract
Melanocyte
stem
cells
(McSCs)
of
the
hair
follicle
are
a
rare
cell
population
within
skin
and
notably
underrepresented
in
whole‐skin,
single‐cell
RNA
sequencing
(scRNA‐seq)
datasets.
Using
enrichment
strategy
to
isolate
KIT+/CD45−
from
telogen
adult
female
C57BL/6J
mice,
we
evaluated
transcriptional
landscape
quiescent
McSCs
(qMcSCs)
at
high
resolution.
Through
this
evaluation,
confirmed
existing
molecular
signatures
for
qMcCS
subpopulations
(e.g.,
Kit+
,
Cd34+/−
Plp1+
Cd274+/−
Thy1+
Cdh3+/−
)
identified
novel
qMcSC
subpopulations,
including
two
that
differentially
regulate
their
immune
privilege
status.
Within
also
predicted
melanocyte
differentiation
potential,
neural
crest
quiescence
depth.
Taken
together,
results
demonstrate
is
heterogeneous
future
studies
focused
on
investigating
changes
qMcSCs
should
consider
subpopulation
composition.
Thyroid
hormone
(TH)
regulates
diverse
developmental
events
and
can
drive
disparate
cellular
outcomes.
In
zebrafish,
TH
has
opposite
effects
on
neural
crest
derived
pigment
cells
of
the
adult
stripe
pattern,
limiting
melanophore
population
expansion,
yet
increasing
yellow/orange
xanthophore
numbers.
To
learn
how
elicits
seemingly
responses
in
having
a
common
embryological
origin,
we
analyzed
individual
transcriptomes
from
thousands
crest-derived
cells,
reconstructed
trajectories,
identified
cell-lineage
specific
to
TH,
assessed
roles
for
receptors.
We
show
that
promotes
maturation
both
cell
types
but
distinct
ways.
melanophores,
drives
terminal
differentiation,
final
xanthophores,
accumulation
orange
carotenoids,
making
visible.
receptors
act
primarily
repress
these
programs
when
is
limiting.
Our
findings
single
endocrine
factor
integrates
very
different
activities
during
generation
form.
Annual Review of Genomics and Human Genetics,
Journal Year:
2019,
Volume and Issue:
20(1), P. 41 - 72
Published: May 18, 2019
Human
skin
and
hair
color
are
visible
traits
that
can
vary
dramatically
within
across
ethnic
populations.
The
genetic
makeup
of
these
traits—including
polymorphisms
in
the
enzymes
signaling
proteins
involved
melanogenesis,
vital
role
ion
transport
mechanisms
operating
during
maturation
distribution
melanosome—has
provided
new
insights
into
regulation
pigmentation.
A
large
number
novel
loci
process
have
been
recently
discovered
through
four
large-scale
genome-wide
association
studies
Europeans,
two
Africans,
one
study
Latin
Americans,
functional
testing
animal
models.
responsible
pigmentation
genes
appear
at
different
population
frequencies,
be
used
as
ancestry-informative
markers,
provide
insight
evolutionary
selective
forces
acted
to
create
this
human
diversity.
Development,
Journal Year:
2019,
Volume and Issue:
146(5)
Published: March 1, 2019
We
review
here
some
of
the
historical
highlights
in
exploratory
studies
vertebrate
embryonic
structure
known
as
neural
crest.
The
study
molecular
properties
cells
that
it
produces,
their
migratory
capacities
and
plasticity,
still-growing
list
tissues
depend
on
presence
for
form
function,
continue
to
enrich
our
understanding
congenital
malformations,
paediatric
cancers
evolutionary
biology.
Developmental
biology
has
been
key
crest,
starting
with
early
days
experimental
embryology
through
today,
when
increasingly
powerful
technologies
contribute
further
insight
into
this
fascinating
cell
population.
Trends in Ecology & Evolution,
Journal Year:
2021,
Volume and Issue:
36(5), P. 430 - 443
Published: Feb. 5, 2021
Melanins
are
widespread
pigments
in
vertebrates,
with
important
roles
visual
signaling,
UV
protection,
and
homeostasis.
Fossil
evidence
of
melanin
melanin-bearing
organelles
-
melanosomes
ancient
vertebrates
may
illuminate
the
evolution
its
functions,
but
macroevolutionary
trends
poorly
resolved.
Here,
we
integrate
fossil
data
current
understanding
function,
biochemistry,
genetics.
Mapping
key
genes
onto
phenotypic
attributes
identifies
potential
genomic
controls
on
evolution.
Taxonomic
anatomical
location,
geometry,
chemistry
vertebrate
linked
to
endothermy.
These
shifts
biology
suggest
fundamental
links
between
melanization
ecology.
Tissue-specific
taxonomic
support
for
evolutionary
tradeoffs
function
cytotoxicity.
Science Advances,
Journal Year:
2022,
Volume and Issue:
8(42)
Published: Oct. 21, 2022
During
embryonic
development,
reciprocal
interactions
between
epidermal
and
mesenchymal
layers
trigger
hair
follicle
morphogenesis.
This
study
revealed
that
microenvironmental
reprogramming
via
control
over
these
enabled
induction
in
vitro.
A
key
approach
is
to
modulate
spatial
distributions
of
epithelial
cells
their
spontaneous
organization.
The
de
novo
follicles
with
typical
morphological
features
emerged
aggregates
the
two
cell
types,
termed
follicloids,
shafts
sprouted
near
100%
efficiency
shaft
length
reached
~3
mm
culture.
Typical
trichogenic
signaling
pathways
were
up-regulated
follicloids.
Owing
replication
morphogenesis
vitro,
melanosome
production
transportation
also
monitored
bulb
region.
vitro
model
might
be
valuable
for
better
understanding
induction,
evaluating
growth
inhibition
by
drugs,
modeling
gray
hairs
a
well-defined
environment.
Nucleic Acids Research,
Journal Year:
2022,
Volume and Issue:
50(5), P. 2493 - 2508
Published: Feb. 11, 2022
Mobile
element
insertions
(MEIs)
are
a
major
class
of
structural
variants
(SVs)
and
have
been
linked
to
many
human
genetic
disorders,
including
hemophilia,
neurofibromatosis,
various
cancers.
However,
MEI
resources
from
large-scale
genome
sequencing
still
lacking
compared
those
for
SNPs
SVs.
Here,
we
report
comprehensive
map
36
699
non-reference
MEIs
constructed
5675
genomes,
comprising
2998
Chinese
samples
(∼26.2×,
NyuWa)
2677
the
1000
Genomes
Project
(∼7.4×,
1KGP).
We
discovered
that
LINE-1
were
highly
enriched
in
centromere
regions,
implying
role
chromosome
context
retroelement
insertion.
After
functional
annotation,
estimated
responsible
about
9.3%
all
protein-truncating
events
per
genome.
Finally,
built
companion
database
named
HMEID
public
use.
This
resource
represents
latest
largest
genomewide
study
on
will
broad
utility
exploration
findings.
