In-silico analysis of E-β-farnesene for its possible insect-repellent activity through interaction with Odorant Binding Proteins (OBPs) DOI Creative Commons
Aqsa Parvaiz,

Hafsa Abid,

Anam Altaf

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract Contrary to humans, many animals, especially insects, rely heavily on the sense of smell detect their foods, predators, mating partners and nesting sites. Odor molecules are chemicals, originating from a source. When such chemical compounds interact with odorant binding proteins (OBPs) subsequently receptors associated insect nervous system, cascade reactions commences. In present study, an in-silicoappraisal broad range aphid repellent was carried out in order propose mechanism action at molecular level. Initially 3-D protein structure prediction using SWISS model, Phyre2 Modeller. The molecule E-beta-farnesene (a compound used as ligand) retrieved PubChem. Physiochemical analyses by Protparam revealed that OBPs basic nature 9.30 isoelectric point (pI) based aliphatic index were found be thermostable. Protein-ligand interaction help Auto Dock Vina (ADV) tool complex interactions among ligand showing affinities different bonds including hydrogen bonding, hydrophobic elastic bonds. However, Phe230 residue OBP Apis gossypii showed ligand’s C9 atom via bond having length 3.04 Å. use new analogous for wide pests should future studies.

Language: Английский

The expansion and loss of specific olfactory genes in relatives of parasitic lice, the stored-product psocids (Psocodea: Liposcelididae) DOI Creative Commons

Jun Sun,

J. Wu, Shengming Sun

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 15, 2025

Booklice, belonging to the genus Liposcelis (Psocodea: Liposcelididae), commonly known as psocids, infest a wide range of stored products and are implicated in transmission harmful microorganisms such fungi bacteria. The olfactory system is critical for insect feeding reproduction. Elucidating molecular mechanisms booklice crucial developing effective control strategies. In this study, we aim bridge knowledge gap by leveraging transcriptome genome data from five species. Using HMMER method manual annotation, have identified common gene families associated with processes, including odorant binding proteins (OBPs), chemosensory (CSPs), receptors (ORs), ionotropic (IRs), sensory neuron membrane (SNMPs). Specifically, 94, 118, 26, 47, 34 olfactory-related genes L. bostrychophila, tricolor, entomophila, decolor, yangi, respectively. Comparison quantities revealed that number ORs IRs significantly higher than those transcriptome. This discrepancy may be due specific expression these certain tissues or their lack during experimental stage. Simultaneously, analysis profiles across different developmental stages varying periods peak genes. These results suggest identification on genome-wide scale more feasible reliable using transcriptome-based approach. Additionally, compared parasitic lice, possess increase inability lice survive without host, whereas habits live complex variable environments. Furthermore, observed IR family tricolor has undergone degree amplification, which facilitate its adaptation diverse environmental conditions. We species first time, providing valuable insights future functional investigations into pheromone recognition. discoveries present promising targets effectively managing psocid pests.

Language: Английский

Citations

0
HcGr76 responds to fructose and chlorogenic acid and is involved in regulation of peptide expression in the midgut of Hyphantria cunea larvae DOI
Jing Sun, Wenjing Zhang,

Zhebo Cui

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(11), P. 5672 - 5683

Published: July 10, 2024

Sensing dietary components in the gut is important to ensure an appropriate hormonal response and metabolic regulation after food intake. The fall webworm, Hyphantria cunea, a major invasive pest China has led significant economic losses ecosystem disruption. larvae's broad host range voracious appetite for leaves make H. cunea primary cause of serious damage both forests crops. To date, however, gustatory receptors (Grs) their regulatory function remain largely unknown.

Language: Английский

Citations

1
Full body sensillar diversity of pumpkin fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae) DOI

Alka Rana,

Dixit Sharma, Himanshu Thakur

et al.

Zoomorphology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Language: Английский

Citations

0
In-silico analysis of E-β-farnesene for its possible insect-repellent activity through interaction with Odorant Binding Proteins (OBPs) DOI Creative Commons
Aqsa Parvaiz,

Hafsa Abid,

Anam Altaf

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract Contrary to humans, many animals, especially insects, rely heavily on the sense of smell detect their foods, predators, mating partners and nesting sites. Odor molecules are chemicals, originating from a source. When such chemical compounds interact with odorant binding proteins (OBPs) subsequently receptors associated insect nervous system, cascade reactions commences. In present study, an in-silicoappraisal broad range aphid repellent was carried out in order propose mechanism action at molecular level. Initially 3-D protein structure prediction using SWISS model, Phyre2 Modeller. The molecule E-beta-farnesene (a compound used as ligand) retrieved PubChem. Physiochemical analyses by Protparam revealed that OBPs basic nature 9.30 isoelectric point (pI) based aliphatic index were found be thermostable. Protein-ligand interaction help Auto Dock Vina (ADV) tool complex interactions among ligand showing affinities different bonds including hydrogen bonding, hydrophobic elastic bonds. However, Phe230 residue OBP Apis gossypii showed ligand’s C9 atom via bond having length 3.04 Å. use new analogous for wide pests should future studies.

Language: Английский

Citations

0