Using Environmental DNA as a Plant Health Surveillance Tool in Forests DOI Open Access

Kirsty Elizabeth McLaughlin,

Hadj Ahmed Belaouni,

A McClure

et al.

Forests, Journal Year: 2025, Volume and Issue: 16(4), P. 632 - 632

Published: April 4, 2025

Plant pests (including pathogens) threaten forests, reduce carbon sequestration, disrupt trade, and are costly to manage. Traditionally, forest surveys involve the visual inspection of trees for symptoms disease; however, this process is time consuming by observed, disease may be widespread. New methods surveillance needed help plant health authorities monitor protect forests from disease. Previous research has shown that metabarcoding environmental DNA (eDNA) can used identify pests. This study collected rainwater samples five sites across Northern Ireland every month a year examine whether eDNA could detect diseases in forests. Metabarcoding internal transcribed spacer (ITS) region was determine fungal oomycete profile passed through canopy spruce, pine, oak, ash trees, along with non-tree field trap. In total, 65 known were detected; seven regulated pests, two had not been previously identified Ireland. work demonstrates programmes.

Language: Английский

Using Environmental DNA as a Plant Health Surveillance Tool in Forests DOI Open Access

Kirsty Elizabeth McLaughlin,

Hadj Ahmed Belaouni,

A McClure

et al.

Forests, Journal Year: 2025, Volume and Issue: 16(4), P. 632 - 632

Published: April 4, 2025

Plant pests (including pathogens) threaten forests, reduce carbon sequestration, disrupt trade, and are costly to manage. Traditionally, forest surveys involve the visual inspection of trees for symptoms disease; however, this process is time consuming by observed, disease may be widespread. New methods surveillance needed help plant health authorities monitor protect forests from disease. Previous research has shown that metabarcoding environmental DNA (eDNA) can used identify pests. This study collected rainwater samples five sites across Northern Ireland every month a year examine whether eDNA could detect diseases in forests. Metabarcoding internal transcribed spacer (ITS) region was determine fungal oomycete profile passed through canopy spruce, pine, oak, ash trees, along with non-tree field trap. In total, 65 known were detected; seven regulated pests, two had not been previously identified Ireland. work demonstrates programmes.

Language: Английский

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