Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiosperm Zostera marina L.: Integrated metabolomic and transcriptomic analysis

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: March 10, 2023

Seed development is a crucial phase in the life cycle of seed-propagated plants. As only group angiosperms that evolved from terrestrial plants to complete their submerged marine environments, mechanisms underlying seed seagrasses are still largely unknown. In present study, we attempted combine transcriptomic, metabolomic, and physiological data comprehensively analyze molecular mechanism regulates energy metabolism Zostera marina seeds at four major developmental stages. Our results demonstrated was reprogrammed with significant alteration starch sucrose metabolism, glycolysis, tricarboxylic acid (TCA cycle), pentose phosphate pathway during transition formation seedling establishment. The interconversion sugar provided storage substances mature further acted as sources support germination growth. glycolysis active Z. establishment, which pyruvate for TCA by decomposing soluble sugar. Notably, biological processes were severely inhibited maturation may have positive effect on germination, maintaining low level metabolic activity preserve viability. Increased acetyl-CoA ATP contents accompanied higher indicating accumulations precursor intermediates metabolite can strengthen facilitate supply large amount oxidatively generated promotes fructose 1,6-bisphosphate synthesis feed back not provides but also complements glycolytic pathway. Collectively, our findings suggest these pathways cooperate each other process transformation maturity transforming tissue highly meet requirement development. These provide insights into roles different perspectives, could habitat restoration meadows via seeds.

Language: Английский

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Testing the relationship among tepal area, length, and width using four Magnolia species

Trees, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 13, 2025

Language: Английский

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Pattern of seed development in rapeseed-mustard genotypes grown under varying seed priming options in lower Gangetic plains of India

Discover Plants., Journal Year: 2025, Volume and Issue: 2(1)

Published: April 8, 2025

Language: Английский

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6-Phosphogluconate dehydrogenase inhibition arrests growth and induces apoptosis in gastric cancer via AMPK activation and oxidative stress

Open Life Sciences, Journal Year: 2023, Volume and Issue: 18(1)

Published: Jan. 1, 2023

Poor outcomes in advanced gastric cancer necessitate alternative therapeutic strategies. 6-Phosphogluconate dehydrogenase (6-PGDH), an enzyme that catalyzes the decarboxylation step oxidative pentose phosphate pathway, has been identified as a promising target many cancers. In this study, we systematically investigated expression and function of 6-PGDH cancer. We found activity were aberrantly elevated tissues compared to their adjacent normal tissues. knockdown using two independent shRNAs resulted minimal levels activity, decreased growth, enhanced cell sensitivity 5-flurorouracil. However, did not affect cells. Mechanistic studies showed inhibition disrupted lipid biosynthesis redox homeostasis cancer, inhibited induced apoptosis. Notably, vitro findings validated vivo xenograft mouse model. This study established is broadly patients can sensitize cells response chemotherapy.

Language: Английский

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Morphological, physiological and biochemical changes in Magnolia zenii Cheng seed during development

Physiologia Plantarum, Journal Year: 2021, Volume and Issue: 172(4), P. 2129 - 2141

Published: May 3, 2021

Abstract An understanding of the seed dynamics endangered plant species, such as Magnolia zenii Cheng, is important for successful conservation. This study examined morphological, physiological and biochemical changes that occur in M. Cheng during development to determine appropriate harvest stage. The appearance seeds was influenced by occurring developmental period, which colour fruits changed from green red, while light brown dark brown. There a significant decrease moisture content 90 120 days after flowering (DAF); however, there no change 135 165 DAF. DAF onwards showed developed cotyledonary embryos. viability exceeded 95% onwards; germination percentage 0, hypothesising might exhibit dormancy under certain conditions this experiment. increase fat, soluble sugar starch approaching maturity. were malate dehydrogenase (MDH), glucose‐6‐phosphate (G‐6‐PDH), 6‐phosphogluconate (6‐PGDH) β‐amylase activities period. At 135–165 DAF, MDH activity remained stable, whereas 6‐PGDH reached its maximum, indicating underwent vigorous metabolism. findings provide theoretical basis researching time.

Language: Английский

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Aging-Induced Reduction in Safflower Seed Germination via Impaired Energy Metabolism and Genetic Integrity Is Partially Restored by Sucrose and DA-6 Treatment

Plants, Journal Year: 2024, Volume and Issue: 13(5), P. 659 - 659

Published: Feb. 27, 2024

Seed storage underpins global agriculture and the seed trade revealing mechanisms of aging is essential for enhancing longevity management. Safflower a multipurpose oil crop, rich in unsaturated fatty acids that are at high risk peroxidation as contributory factor to aging. However, molecular responsible safflower viability loss not yet elucidated. We used controlled deterioration (CDT) conditions 60% relative humidity 50 °C reduce germination freshly harvested seeds analyzed aged using biochemical techniques. While malondialdehyde (MDA) acid content increased significantly during CDT, catalase activity soluble sugar decreased. KEGG analysis gene function qPCR validation indicated severely impaired several key functional biosynthetic pathways including glycolysis, metabolism, antioxidant activity, DNA replication repair. Furthermore, exogenous sucrose diethyl aminoethyl hexanoate (DA-6) treatment partially promoted seeds, further demonstrating vital role metabolism recovery processes. concluded energy genetic integrity aging, which contributes vigor. Such metabolic degradation, tricarboxylic cycle (TCA) impaired, especially produced by hydrolysis triacylglycerols they efficiently converted via glyoxylate provide supply seedling growth. At same time, reduced capacity nucleotide synthesis repair ability aggravate damage DNA, reducing vitality.

Language: Английский

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Adaptations of Rice Seed Germination to Drought and Hypoxic Conditions: Molecular and Physiological Insights

Seeds, Journal Year: 2024, Volume and Issue: 3(4), P. 656 - 676

Published: Dec. 2, 2024

Seed germination is crucial for plant survival, crop stand establishment, and achieving optimal grain yield. The main objective of this review to explore the physiological molecular mechanisms governing rice seed under aerobic (water stress) anaerobic (hypoxic) conditions in direct-seeded (DSR) systems. Moreover, it discusses recent genomic advancements innovations improve germination. Here, we discuss how coleoptile mesocotyl elongation plays a vital role (AG) function raised antioxidants, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) maintaining Reactive Oxygen Species (ROS), malondialdehyde (MDA) homeostasis stabilizing water-scarce conditions. This study comprehensively highlights functions dynamics phytohormones—GA (gibberellic acid) ABA (abscisic acid)—key regulatory genes, transcription factors (TFs), key proteins, metabolic pathways, glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA), regulating both Conventional agronomic cultural practices, such as selection, priming, coating, hardening, have proven utilization novel approaches—such clustered regularly interspaced short palindromic repeat (CRISPR-Cas9) mediated genome editing, marker-assisted selection (MAS), genome-wide associations studies (GWAS), single nucleotide polymorphisms (SNPs), multi-omics, RNA sequencing—combined with beneficial quantitative trait loci (QTLs) has expanded knowledge genomics inheritance. These aid development specific traits enhancing DSR.

Language: Английский

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Influence of Seed Development and Maturation on the Physiological and Biochemical Seed Quality

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 17, 2024

Seed quality is one of the widely discussed topics in seed system and biology; thus, many countries with functional vibrant have invested heavily assurance control. Good crucial for any cropping system, without it, there poor field establishment wastage other production inputs. responds well to added inputs, ensures uniform crop establishment, has higher yield advantage under same management practice. It is, however, important note that influenced greatly by development maturation. Storage reserves are deposited storage tissues during maturation, these early stages germination maintenance seedling life when it not yet developed good photosynthetic capacity. The stage at which harvested enormous influence on its performance either or storage, terms behavior vigor characteristics, viability. This chapter presents some current understandings findings emphasis physiological biochemical quality.

Language: Английский

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Effect of Steam Explosion on the Morphological, Textural and Chemical Composition Characteristics of Betel Nut

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: March 30, 2023

Abstract To reduce the adverse physical effects on oral mucosa caused by excessive hardness of betel nut fibres, steam explosion was used to soften nuts. The effect 3 operating parameters (pressure holding time, pressure and initial moisture content) morphology, texture chemical composition nuts investigated. fibre Shore decreased 56.17%-89.28% 7.03%-34.29%, respectively, transverse tensile strength also up 60.72% 24.62%, respectively. Moreover, coefficient friction content increased. After explosion, increased in diameter, became darker more yellow‒red colour, showed a damaged microstructure. contents lignin, hemicellulose, free phenol, bound phenol alkaloids after treatment, with total phenols decreasing from 44.07 mg(GAE)/g 7.84 mg/g 30.61 6.50 mg/g, A-50 s treatment condition. A slight increase cellulose observed when conditions exceeded 80 or 0.75 MPa. quantity phenols, soluble solids released under same simulated release conditions. research that efficiency positively correlated time pressure, while reduced efficiency. Therefore, is an effective pretreatment approach facilitate healthy development industry.

Language: Английский

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Biochemical Changes during Fruit and Seed Development in Nanjing Linden (Tilia miqueiana M.)

Forests, Journal Year: 2023, Volume and Issue: 14(5), P. 969 - 969

Published: May 8, 2023

In China, the wild population of Nanjing Linden (Tilia miqueliana M.) is experiencing a drastic decline, primarily due to high levels empty seeds. This study aimed measure biochemical and physiological changes during fruit seed development in T. determine developmental mechanism. The weight method photosynthetic respiration were used biological aspects both fruits embryos, while transmission electron microscopy anthrone colorimetric endosperm content, including sugar, starch, protein, fat. Enzyme-linked immunosorbent assays conducted endogenous plant hormones such as indole-3-acetic acid (IAA), gibberellic 3 (GA3), zeatin riboside (ZR), abscisic (ABA). nonlinear least-squares was fit model nutrient hormone levels, revealing that size expanded from 5th 65th day moisture content exhibited downward trend, along with decrease intensity. Embryos found be fully developed between 35 DAF 65 DAF, nutrients endosperm, i.e., fat, continuously accumulated after 50 DAF. Additionally, ABA, IAA, GA3, ZR contents synergistically regulate maturation.

Language: Английский

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