Comparative physiological and co-expression network analysis reveals potential hub genes and adaptive mechanisms responsive to NaCl stress in peanut (Arachis hypogaea L.)

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 6, 2025

Salt stress has become a major threat to peanut yield and quality, salt is particularly detrimental seedling growth. Combined analysis of the physiology transcriptomics salt-tolerant variety (NH5) salt-sensitive (FH23) under 200 mM NaCl was conducted identify key factors influencing differences in tolerance investigate potential regulatory mechanisms hub genes associated with peanuts. Malondialdehyde (MDA) content electrolyte leakage rate were significantly increased prolonged stress, increase FH23 being even more pronounced. NH5 maintained intracellular osmotic homeostasis by accumulating free proline soluble protein content. In addition, exhibited higher antioxidant enzyme activity. The net photosynthetic (Pn) decreased 64.24% 94.49% after 96 h stress. intercellular CO2 concentration (Ci) 7.82%, while that 42.74%. This suggests non-stomatal limiting primary cause decline photosynthesis observed FH23. Transcriptome revealed presence 12,612 differentially expressed (DEGs) response exhibiting greater number than NH5. upregulated downregulated at 24 whereas exceeded 48 h. Subsequently, Weighted Gene Co-expression Network Analysis (WGCNA) performed conjunction physiological data. Twenty-four identified, which encoded delta-1-pyrroline-5-carboxylate synthase, aldehyde dehydrogenase, SNF1-related kinase, magnesium transporter, temperature-induced lipocalin-1, ERF transcription factors. A network for peanuts been constructed. findings distinct identified candidate further investigation.

Language: Английский

---

Multiomics Combined with Expression Pattern Analysis Reveals the Regulatory Response of Key Genes in Potato Jasmonic Acid Signaling Pathways to Cadmium Stress

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Jasmonic acid (JA) is an endogenous phytohormone that regulates plant physiological metabolism and stress response processes, either independently or through hormone crosstalk. Our assay transcriptome-metabolome analysis revealed the key genes metabolites involved in JA pathway to 0-250 μM cadmium (Cd) potato seedlings. Transcriptome gene set enrichment ontology indicated JA-related were significantly enriched. Specifically, members from

Language: Английский

---

Genome-wide identification and expression analysis of TPP gene family under salt stress in peanut (Arachis hypogaea L.)

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0305730 - e0305730

Published: July 18, 2024

Trehalose-6-phosphate phosphatase (TPP), a key enzyme for trehalose biosynthesis in plants, plays pivotal role the growth and development of higher as well their adaptations to various abiotic stresses. Employing bioinformatics techniques, 45 TPP genes distributed across 17 chromosomes were identified with conserved Trehalose-PPase domains peanut genome, aiming screen those involved salt tolerance. Collinearity analysis showed that 22 from formed homologous gene pairs 9 Arabidopsis 31 soybean, respectively. Analysis cis-acting elements promoters revealed presence multiple hormone- stress-responsive promoter regions AhTPP s. Expression pattern members family responded significantly stresses, including low temperature, drought, nitrogen deficiency, exhibited certain tissue specificity. Salt stress upregulated s, number responsive observed at seedling stage compared podding stage. The intuitive physiological effect was reflected accumulation content leaves plants under control. These findings indicate crucial peanut’s response laying foundation further functional studies utilization these genes.

Language: Английский

---

Integrative Analysis of Multi Omic Data

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

---

Drought Stress Inhibits the Accumulation of Rotenoids and the Biosynthesis of Drought-Responsive Phytohormones in Mirabilis himalaica (Edgew.) Heim Calli

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1644 - 1644

Published: Dec. 21, 2024

Background: Mirabilis himalaica, distributed in the high-altitude, arid, and semi-arid regions of Xizang, exhibits great tolerance to drought, which is rich rotenoids other secondary metabolites. It still unknown, though, how drought stress influences rotenoid synthesis M. himalaica. Methods: In this study, calli himalaica were subjected 5% PEG6000 for 0, 20, 40 h divided into control group (CK), mild-drought-treated (M), high-drought-treated (H), respectively. We then analyzed relative content three main using high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC-ESI-MS/MS). Results: Our findings demonstrated that was significantly reduced under stress. Transcriptome analysis subsequently revealed 14,525 differentially expressed genes (DEGs) between different treatments. Furthermore, these DEGs exhibited enrichment pathways associated with isoflavone biosynthesis hormone signaling pathways. Key decreased expression patterns during also found be involved accumulation drought-responsive phytohormone signaling, including abscisic acid (ABA), auxin (IAA), jasmonic (JA). Conclusions: These elucidate molecular processes resistance shed light on relationship production

Language: Английский

---