Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish DOI Creative Commons

Xinyan Zhu,

Yang Chen, Xinliang Zhang

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(5), P. 227 - 227

Published: May 15, 2025

In the field of fish genetic breeding, accurately determining DNA content and ploidy is great significance. This article introduces use flow cytometry (FCM) to measure conduct analysis by sampling different tissues freshwater species. It describes FCM detection methods their effectiveness for individual tissues. These include embryos fry, as well blood, caudal fins, sperm adult live fish, also specific such testes, ovaries, gills, spleens, livers under anatomical conditions. Moreover, application or individuals in stages practice breeding analyzed. research covers samples from growth a variety tissue types. The results show that this method exhibits high stability reliability samples, providing solid data support subsequent research. holds significant value breeding.

Language: Английский

Novel screening of molecular markers reveals genetic basis for heterosis in hybrids from red crucian carp and white crucian carp DOI
He Zhang, Ru Li, Yun Zeng

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742098 - 742098

Published: Jan. 1, 2025

Language: Английский

Citations

1
Study on the Biological Characteristics of a Novel Intergeneric Hybrid Fish Derived from Megalobrama Amblycephala (♀) × Culter Dabryi (♂) DOI
Zhifeng Zhou,

Xinge Ouyang,

Chang Wu

et al.

Published: Jan. 1, 2025

Distant hybridization in fish typically involves the combination of genetic material from two distantly related species, creating new hybrids with substantial alterations both phenotypic traits and molecular genetics. In this study, we successfully produced a hybrid (BG) through intergeneric between blunt snout bream (Megalobrama amblycephala, BSB, 2n=48, ♀) green tip culter (Culter dabryi, GTC, ♂). We analyzed DNA content, chromosomal numbers, karyotypes, morphological traits, fertility, 5S rDNA, Sox genes, mitochondrial genome DNA, muscle nutritional composition GTC BG. The flow cytometry analysis demonstrated that BG was an allodiploid 48 chromosomes. Fertility revealed exhibited bisexual capable reproduction male female gametes. With regard to growth, possessed growth rate faster than GTC. Muscle indicated had higher levels crude protein umami amino acids its parents, it rich polyunsaturated fatty were beneficial human health. Genetic rDNA genomic predominantly inherited characteristics certain gene fragments derived speculating occurrence interactions heterologous genes. Additionally, Sox9 sequencing further suggested may have distinct sets material, one each parent. integration biparental elements hypothesized enhance BG's regulatory efficiency during developmental reproductive processes. conclusion, exhibits superior making promising germplasm resource for future breeding. Its potential applications are improvement development aquaculture.

Language: Английский

Citations

0
First successful artificial distant hybridization of softshell turtle between Palea steindachneri and Pelodiscus sinensis DOI
Xiaofei Ma, Karsoon Tan, Mengting He

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742329 - 742329

Published: Feb. 1, 2025

Language: Английский

Citations

0
Assessment of Germplasm Improvement in Three Farmed Grass Carp Populations Based on Genetic Variability DOI Creative Commons

Zhongyuan Shen,

Shao Li-ming,

Xixi Liu

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(3), P. 230 - 230

Published: Feb. 25, 2025

The assessment of genetic improvement was comprehensively analyzed using the mtDNA Cyt b gene and SSR markers among three farmed grass carp populations caught in May 2024. results an analysis 198 individuals showed that haplotype diversity index (Hi) nucleotide (Pi) were 0.555 0.00058, respectively. marker data 196 indicated unequal dosage amplification at same locus found CC population. Moreover, total number alleles (A: 338), per (Na: 15.36), observed heterozygosity (Ho: 0.8391), expected (He: 0.8380), polymorphic information content (PIC: 0.8191) KC population relatively higher than 129; Na: 5.86; Ho: 0.0025; He: 0.6191; PIC: 0.5747) CY 293; 8.77; 0.821; 0.7483; populations. FST AMOVA existence a significant differentiation (p < 0.001), with high between In summary, variability exists population, while father (CY) mother (CC) have low variability. This study reveals evidence “micro-hybrid”. demonstrated combining both gynogenesis backcross breeding technology is vital for carp. continuous research into health these required as well support protection germplasm resources artificial breeding.

Language: Английский

Citations

0
Comparison of testicular development of hybrid crucian carp (Japanese white crucian carp ♀ × red crucian carp ♂) in traditional pond, lotus pond and rice paddy DOI

Weiling Qin,

Tao Dai,

Jiamin Pi

et al.

Reproduction and Breeding, Journal Year: 2025, Volume and Issue: 5(2), P. 27 - 36

Published: March 27, 2025

Language: Английский

Citations

0
Dietary Tea Polyphenols Alleviate Acute-Heat-Stress-Induced Death of Hybrid Crucian Carp HCC2: Involvement of Modified Lipid Metabolisms in Liver DOI Creative Commons
Na Zhang, Jun Tao, Qifang Yu

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(4), P. 229 - 229

Published: March 27, 2025

Global warming poses significant challenges to aquaculture, as elevated water temperatures adversely affect fish health and survival. This study investigated the effects potential mechanisms of dietary tea polyphenols (TPs) on acute heat stress survival in hybrid crucian carp HCC2. The control (CON) group (HS group, three replicates, each containing 20 fish, n = 60 per group) were fed diets with 0 mg/kg TPs, experimental groups (HSLTP, HSMTP, HSHTP, × 3 replicates) 100, 200, or 400 TPs for days. Further, (HS, HSLTP, HSHTP) exposed at 38 °C 24 h induce stress. Survival data serum tissue samples collected analysis. Metabolomics using UPLC-Q-TOF/MS was employed evaluate metabolite changes livers. Notably, significantly improved rates antioxidant enzyme levels reduced ALT, AST, cortisol, glucose, MDA, liver HSP-70 heat-stressed fish. Metabolomic analysis revealed that modulated lipid metabolism, particularly glycerophospholipid arachidonic acid pathways, which may contribute a higher tolerance These findings suggest are promising, eco-friendly feed additive protecting from optimizing aquaculture practices.

Language: Английский

Citations

0
The formation of allodiploid hybrids derived from Labeo rohita ♀ × Cyprinus carpio ♂ and exhibiting stronger low temperature tolerance DOI

Jiali Lin,

Haoran Zhang,

Jietao Wang

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742498 - 742498

Published: March 1, 2025

Language: Английский

Citations

0
Establishment and Characteristics of the Spermatogonial Stem Cell Line from the Yellow River Carp (Cyprinus carpio haematopterus) DOI Creative Commons

Huijie Zhou,

Tianqi Liu, T. Zhang

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 536 - 536

Published: May 12, 2025

To address the growing consumer demands for improved fish meat quality, desirable morphological traits, and sustainable production practices, researchers have intensified efforts in selective breeding genetic improvement of carp (Cyprinus carpio) varieties. However, traditional methods are often time-consuming inefficient, which poses challenges to development aquaculture industry. The establishment germ stem cell lines offers a crucial tool study cells, improvement, species conservation. In this study, we successfully established spermatogonial line (YRSSCs) from Yellow River carpio haematopterus) that can be cultured vitro long term. We optimized culture conditions maintain their self-renewal differentiation capabilities. results demonstrated YRSSCs diploid karyotype stably proliferate over year L-15 medium supplemented with 5 mmol/L HEPES, 50 μmol/L β-mercaptoethanol, 15% FBS, 2 ng/mL bFGF, LIF, 1% serum, 800 IU/mL penicillin, 0.8 mg/mL streptomycin, μg/mL amphotericin B, zebrafish embryo extract, glutamine at 30 °C absence CO2. cells exhibited typical gene expression profile, strong vasa, plzf-a, Oct4-a genes. Additionally, found possess ability differentiate functionally colonize vivo within recipient bodies. This research explored potential both vivo, providing novel strategy through cell-based editing transplantation technologies.

Language: Английский

Citations

0
Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish DOI Creative Commons

Xinyan Zhu,

Yang Chen, Xinliang Zhang

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(5), P. 227 - 227

Published: May 15, 2025

In the field of fish genetic breeding, accurately determining DNA content and ploidy is great significance. This article introduces use flow cytometry (FCM) to measure conduct analysis by sampling different tissues freshwater species. It describes FCM detection methods their effectiveness for individual tissues. These include embryos fry, as well blood, caudal fins, sperm adult live fish, also specific such testes, ovaries, gills, spleens, livers under anatomical conditions. Moreover, application or individuals in stages practice breeding analyzed. research covers samples from growth a variety tissue types. The results show that this method exhibits high stability reliability samples, providing solid data support subsequent research. holds significant value breeding.

Language: Английский

Citations

0