The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality DOI Creative Commons
Larbi Allai, Xavier Druart, Pınar Terzioğlu

et al.

Veterinary Medicine International, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 9

Published: Jan. 1, 2023

Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context, first aim study was determine antioxidant activity and composition (minerals, vitamins, sugars) Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose investigate effect ETHEX supplementation liquid ram extended with skim milk (SM) at 5°C. The studied using free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH•) assay. mineral sugar vitamin contents were determined an inductively coupled plasma optical emission spectrometry (ICP-OES) HPLC-DAD-RID analytical instruments. As second part, collected from five Boujaâd rams vagina. ejaculates more than 70% motility pooled, extender without (control) or supplemented 1–8% (37°C; 0.8 × 109 sperm/mL). Sperm parameters assessed 8, 24, 48, 72 h. results showed that had higher compared those ascorbic acid butylated hydroxytoluene (BHT). Furthermore, contains considerable amount minerals, sugars. inclusion 1 2% in SM increased sperm motility, viability, membrane integrity decreased abnormality spontaneous catalyzed lipids peroxidation ( p<0.05 ) up addition, diluted level DNA fragmentation control group id="M2">p<0.05 ). conclusion, could be recommended spermatozoa. However, its effects insemination should studied.

Language: Английский

Heat stress and ram semen production and preservation: Exploring impacts and effective strategies DOI
Anass Ben Moula,

Zineb Moussafir,

Naima Hamidallah

et al.

Journal of Thermal Biology, Journal Year: 2024, Volume and Issue: 119, P. 103794 - 103794

Published: Jan. 1, 2024

Language: Английский

Citations

4
Investigation of chia seed (Salvia hispanica L.) extract supplementation to extender on post-thawing ram sperm parameters and genes-related antioxidant capacity DOI
Wael A. Khalil, Aya A. Ismail,

M. El-Harairy

et al.

Small Ruminant Research, Journal Year: 2024, Volume and Issue: 239, P. 107345 - 107345

Published: Aug. 30, 2024

Language: Английский

Citations

2
Effect of C-phycocyanin purified from Spirulina platensis on cooled ram semen quality and in vivo fertility DOI
Abdellatif Rahim,

Saad Salhi,

Nora El Khelfaoui

et al.

Theriogenology, Journal Year: 2023, Volume and Issue: 215, P. 234 - 240

Published: Dec. 9, 2023

Language: Английский

Citations

5
Exploring Endogenous and Exogenous Factors for Successful Artificial Insemination in Sheep: A Global Overview DOI Creative Commons
Bouchra El Amiri, Abdellatif Rahim

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(2), P. 86 - 86

Published: Feb. 11, 2024

Artificial insemination (AI) plays a vital role in animal breeding programs. AI is applied to enhance genetics and facilitate the widespread integration of desirable characteristics with high potential for productivity. However, sheep, this biotechnology not commonly practicable due multi-factorial challenges, resulting inconsistent outcomes unpredictable results. Thoughtful selection semen donors recipients based on genetic merit deeply impacts ovine outcomes. Additionally, endogenous factors such as breed, age, fertility traits, disorders, cervical anatomy ewes contribute success. Extensive research has studied exogenous influences sexual behavior, reproductive health, hormonal regulation, all impacting These include techniques like estrus induction, synchronization, handling methods (fresh/chilled/frozen), (cervical/laparoscopic), well nutritional climatic conditions. This overview literature highlights challenges facing successful proposes strategies best practices improvement. paper will serve guide understanding optimizing success AI.

Language: Английский

Citations

1
Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination DOI
Bouchra El Amiri, Anass Ben Moula,

Nora El Khelfaoui

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(8), P. 1132 - 1138

Published: June 22, 2023

This study aimed to investigate the effectiveness of Duragen® and skimmed milk (SM) extenders on quality parameters, bacterial load fertilization ability stored ram semen. A total 50 ejaculates from Sardi rams (n = 5) aged 2.5-3 years, were collected in SM at 15°C. The motilities velocity parameters generated by CASA system then evaluated 0, 8 24 h storage. Afterward, loads sperm extended determined 5 incubation. In addition, ewes 100) 2 have been chosen same herd. selected synchronized inseminated using semen for results revealed that progressive motilities, straight (VSL), straightness (SRT), lateral head displacement (ALH) beat cross frequency (BCF) not affected extender type after storage (p > .05). However, curvilinear (VCL), average path (VAP), linearity (LIN) wobble (WOB) showed higher values compared with < Bacterial observed mainly (183 UFC/mL) (357 only case showing a is when time attains (199 UFC/mL). Concerning fertility, diluted both resulting high fertility rates which reaches 66% 73% SM, respectively, no statistical difference summary, decreased maintained fertility. These findings suggest could be used as alternative ovine artificial insemination (OAI).

Language: Английский

Citations

2
Adjusting ram semen preservation: Exploring the impact of oxygen exposure during liquid storage DOI
Anass Ben Moula,

Naima Hamidallah,

Abdelmoughit Badi

et al.

Reproduction in Domestic Animals, Journal Year: 2024, Volume and Issue: 59(5)

Published: May 1, 2024

Abstract This study investigated the effects of storage conditions on quality chilled ram semen stored at 4°C for 48 h, comparing aerobic and anaerobic conditions. Ejaculates from INRA180 rams were collected under both conditions, with assessments 0‐, 24‐, 48‐h intervals. Various sperm parameters examined, including motility, velocity, viability, morphology, membrane integrity, lipid peroxidation. Results showed that duration significantly impacted quality, leading to a gradual decline 0 24 h h. Notably, after initial progressive motility (PM) integrity (MI) demonstrated distinct responses Anaerobic consistently improved PM MI values compared between also enhanced viability reduced abnormality mark. Total remained stable throughout storage. Velocity (VCL: curvilinear velocity; VSL: straight velocity VAP: average path) exhibited differences 24‐ intervals, resulting in higher VAP VSL values. Moreover, peroxidation increase independent Remarkably, yielded lower levels storage, regardless duration. In conclusion, this highlights proved advantageous particularly

Language: Английский

Citations

0
The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality DOI Creative Commons
Larbi Allai, Xavier Druart, Pınar Terzioğlu

et al.

Veterinary Medicine International, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 9

Published: Jan. 1, 2023

Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context, first aim study was determine antioxidant activity and composition (minerals, vitamins, sugars) Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose investigate effect ETHEX supplementation liquid ram extended with skim milk (SM) at 5°C. The studied using free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH•) assay. mineral sugar vitamin contents were determined an inductively coupled plasma optical emission spectrometry (ICP-OES) HPLC-DAD-RID analytical instruments. As second part, collected from five Boujaâd rams vagina. ejaculates more than 70% motility pooled, extender without (control) or supplemented 1–8% (37°C; 0.8 × 109 sperm/mL). Sperm parameters assessed 8, 24, 48, 72 h. results showed that had higher compared those ascorbic acid butylated hydroxytoluene (BHT). Furthermore, contains considerable amount minerals, sugars. inclusion 1 2% in SM increased sperm motility, viability, membrane integrity decreased abnormality spontaneous catalyzed lipids peroxidation ( p<0.05 ) up addition, diluted level DNA fragmentation control group id="M2">p<0.05 ). conclusion, could be recommended spermatozoa. However, its effects insemination should studied.

Language: Английский

Citations

0