Assessing spatial mating patterns in translocated populations of Campanula glomerata DOI Creative Commons
Fabienne Van Rossum,

Sarah Le Pajolec,

Cécile Godé

et al.

Global Ecology and Conservation, Journal Year: 2023, Volume and Issue: 46, P. e02548 - e02548

Published: June 19, 2023

Plant translocations represent a solution for rescuing nonviable populations and recreating population networks. An essential criterion translocation success is genetic diversity enhancement or maintenance in post-translocation generations. Genetic mixing, i.e. individual relatedness spatial structure, also needs to be considered. We investigated the processes involved patterns of first generation (recruits seed progeny) translocated insect-pollinated, self-incompatible herb Campanula glomerata. combined direct indirect estimates contemporary mating gene dispersal using 15 microsatellite loci estimate clonal extent, reconstruct sibship parentage pollen distances. representative natural seed-source populations. Combining sources enhanced effective number breeders (Ne = 102–175). Clonal propagation was only marginal. Pollen occurred at short distances within (up 18 m), favouring fine-scale structure (SGS). This means that diversity, random high admixture levels are ascribed mixed arrangement transplants. Stronger SGS might lead biparental inbreeding reduced set due lack compatible neighbours, impact long-term sustainability. Increasing seed-dispersal among sites mediated by grazing livestock used grassland management. Some long-distance flow events (0.68–2.35 km) between were detected, suggesting potential creating large restore connectivity flow.

Language: Английский

Easy‐to‐use R functions to separate reduced‐representation genomic datasets into sex‐linked and autosomal loci, and conduct sex assignment DOI Creative Commons
Diana A. Robledo‐Ruiz, Lana Austin, J. Nevil Amos

et al.

Molecular Ecology Resources, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 1, 2023

Identifying sex-linked markers in genomic datasets is important because their presence supposedly neutral autosomal can result incorrect estimates of genetic diversity, population structure and parentage. However, detecting loci be challenging, available scripts neglect some categories variation. Here, we present new R functions to (1) identify separate ZW XY sex determination systems (2) infer the individuals based on these loci. We tested data for two bird one mammal species compared biological inferences made before after removing using our function. found that function identified with ≥98.8% accuracy an average 87.8%. showed standard filters, such as low read depth call rate, failed remove up 54.7% This led (i) overestimation FIS by 24%, number private alleles 8%; (ii) wrongly inferring significant differences heterozygosity; (iii) obscuring (iv) ~11% fewer correct parentages. discuss how failure lead (e.g. sex-biased dispersal cryptic structure) misleading management recommendations. For reduced-representation at least 15 known-sex each sex, offer convenient resources remaining (freely https://github.com/drobledoruiz/conservation_genomics).

Language: Английский

Citations

8
Reproductive characteristics, population genetics, and pairwise kinship inform strategic recovery of a plant species in a fragmented landscape DOI Creative Commons
Chantelle Doyle, Jia‐Yee S. Yap, Jason G. Bragg

et al.

Conservation Science and Practice, Journal Year: 2023, Volume and Issue: 5(4)

Published: Feb. 22, 2023

Abstract Population genetics and understanding of mating systems provide fundamental information for conservation planning. Pairing these methods is a powerful tool in the study threatened species, however, they are rarely applied concert. We examined system used molecular to measure pairwise kinship potential inbreeding Hibbertia spanantha , critically endangered long‐lived shrub endemic Sydney Basin, Australia, as model planning species highly fragmented populations. In situ hand pollination experiments demonstrated that preferentially outcrossing, with limited ability self‐pollinate (either autogamously or geitonogamously). Although population confirmed high levels clonality, there currently enough heterozygosity successful open pollination, primarily through buzz by Sweat Bees ( Lasioglossum [Chilalictus ]). High clonality one may be cause reduced fitness, identified because our outcrossing treatment produced significantly more seeds greater viability seed mass than treatments. Differences weight filled (viable) were between populations, although not treatments, where clonal dominance swamping pollinator foraging activities. Identification system, reproductive capacity, impacts fragmentation on genetic health provides robust basis strategic this including establishment an ex rescue augmentation. These easily applicable particularly relevant other plant small populations those occurring systems.

Language: Английский

Citations

4
Genetic diversity assessment of Helichrysum arenarium (Asteraceae) for the genetic restoration of declining populations DOI Creative Commons
Fabienne Van Rossum, Cécile Godé, Alenka Baruca Arbeiter

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(2)

Published: Feb. 1, 2024

(L.) Moench (Asteraceae) is a self-compatible, insect-pollinated herb occurring in sand grasslands, and declining endangered many parts of its European distribution range. A recovery plan

Language: Английский

Citations

1
Maternal effects and inbreeding depression in post‐translocation progeny of Campanula glomerata DOI
Fabienne Van Rossum,

Sarah Le Pajolec

Plant Biology, Journal Year: 2024, Volume and Issue: 26(3), P. 427 - 436

Published: March 1, 2024

Evaluation of plant translocation success based on fitness-related quantitative traits combined with molecular markers may contribute to a finer assessment inbreeding, selective and rescue processes, which might have long-term consequences for population dynamics viability. We investigated fitness (seed germination, seedling viability, juvenile growth mortality) 15 microsatellite loci the first post-translocation seed progeny from two translocated populations Campanula glomerata, an insect-pollinated, self-incompatible perennial herb. examined whether heterosis through admixture, site maternal transplant source origin lineage affect quality in controlled cultivation conditions. Flower production germination transplants was higher one sites, be related differences soil vegetation composition cover. Strong effects were found size, largest producing progeny. The rosette diameter maintained across whole period measured. There inbreeding depression (rather than heterosis) biparental at early stage, also expressed mortality. Our findings highlight that origin, especially when sources consisted small, fragmented remnants, value generations. If persist, they global genetic diversity patterns long term. Further admixture next generations buffer or lead outbreeding depression.

Language: Английский

Citations

1
Easy-to-use R functions to separate reduced-representation genomic datasets into sex-linked and autosomal loci, and conduct sex-assignment DOI Open Access
Diana A. Robledo‐Ruiz, Lana Austin, J. Nevil Amos

et al.

Authorea (Authorea), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 2, 2022

Identifying sex-linked markers in genomic datasets is important, because their analyses can reveal sex-specific biology, and presence supposedly neutral autosomal result incorrect estimates of genetic diversity, population structure parentage. But detecting loci be challenging, available scripts neglect some categories variation. Here, we present new R functions to (1) identify separate ZW XY sex determination systems (2) infer the individuals based on these loci. Two additional are presented, (3) remove with artefactually high heterozygosity, (4) produce input files for parentage analysis. We test data two sexually-monomorphic bird species, including one a neo-sex chromosome system, by comparing biological inferences made before after removing using our function. found that standard filters, such as low read depth call rate, failed up 28.7% This led (i) overestimation FIS ≤ 9%, number private alleles 8%; (ii) wrongly inferring significant sex-differences (iii) obscuring structure, (iv) ~11% fewer correct parentages. discuss how failure lead (e.g., sex-biased dispersal cryptic structure) misleading management recommendations. For reduced-representation at least 15 known-sex each sex, offer convenient, easy-to-use resources avoid this, remaining individuals.

Language: Английский

Citations

5
Assessing spatial mating patterns in translocated populations of Campanula glomerata DOI Creative Commons
Fabienne Van Rossum,

Sarah Le Pajolec,

Cécile Godé

et al.

Global Ecology and Conservation, Journal Year: 2023, Volume and Issue: 46, P. e02548 - e02548

Published: June 19, 2023

Plant translocations represent a solution for rescuing nonviable populations and recreating population networks. An essential criterion translocation success is genetic diversity enhancement or maintenance in post-translocation generations. Genetic mixing, i.e. individual relatedness spatial structure, also needs to be considered. We investigated the processes involved patterns of first generation (recruits seed progeny) translocated insect-pollinated, self-incompatible herb Campanula glomerata. combined direct indirect estimates contemporary mating gene dispersal using 15 microsatellite loci estimate clonal extent, reconstruct sibship parentage pollen distances. representative natural seed-source populations. Combining sources enhanced effective number breeders (Ne = 102–175). Clonal propagation was only marginal. Pollen occurred at short distances within (up 18 m), favouring fine-scale structure (SGS). This means that diversity, random high admixture levels are ascribed mixed arrangement transplants. Stronger SGS might lead biparental inbreeding reduced set due lack compatible neighbours, impact long-term sustainability. Increasing seed-dispersal among sites mediated by grazing livestock used grassland management. Some long-distance flow events (0.68–2.35 km) between were detected, suggesting potential creating large restore connectivity flow.

Language: Английский

Citations

2