Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral Galaxea fascicularis: Revealing Key Proteins Related to Gamete Maturation and Fertilization DOI Creative Commons
Yinyin Zhou,

Jingzhao Ke,

Lishu Zheng

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(12), P. 2341 - 2341

Published: Dec. 20, 2024

Sexually propagated scleractinian corals are in high demand for coral reef restoration. However, threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck large-scale cultivation sexually reproducing corals. In this study, we analyzed proteomic signatures red eggs and spermatozoa from ecologically significant Galaxea fascicularis, using data-independent acquisition mass spectrometry (DIA-MS) method. A total 7741 7279 proteins mature were identified, respectively. Among these proteins, 596 spermatozoa-specific 1056 egg-specific. Additionally, 4413 differentially abundant (DAPs) among 3121 up-regulated 1292 spermatozoa. Furthermore, anenrichment analyses showed that DAPs identified mainly involved progesterone-mediated oocyte maturation pathway lectin pathway; detected insulin secretion metabolic pathways generation energy. This result will contribute discovery intrinsic regulation gamete fertilization. at least 57 associated with including fluorescent protein (RFP), vitellogenin (VG), egg (EP), testis-specific serine/threonine-protein kinase family (TSSKs), EF-hand Ca2+-binding (EFHC1 EFHC2). Particularly, third yolk EUPHY was reported first time G. fascicularis. conclusion, study unveiled groundbreaking insights into sexual reproduction, paving way more effective conservation sustainable development ecosystems

Language: Английский

Survival and growth of “branches of opportunity” from ten coral species outplanted on framed reef modules DOI
Junling Zhang,

Jingzhao Ke,

Xiangbo Liu

et al.

Ecological Engineering, Journal Year: 2025, Volume and Issue: 212, P. 107529 - 107529

Published: Jan. 21, 2025

Language: Английский

Citations

0
The impact of environmental conditions on the heat tolerance of Acropora hyacinthus DOI
Hongmin Wang, He Zhao, Wentao Zhu

et al.

Journal of Experimental Marine Biology and Ecology, Journal Year: 2025, Volume and Issue: 585, P. 152096 - 152096

Published: March 6, 2025

Language: Английский

Citations

0
An integrative analysis of proteomics and metabolomics reveals the effects of active restoration on Acropora hyacinthus DOI Open Access
He Zhao, Xiangbo Liu, Junling Zhang

et al.

Journal of Experimental Marine Biology and Ecology, Journal Year: 2023, Volume and Issue: 570, P. 151972 - 151972

Published: Nov. 8, 2023

Language: Английский

Citations

4
Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral Galaxea fascicularis: Revealing Key Proteins Related to Gamete Maturation and Fertilization DOI Creative Commons
Yinyin Zhou,

Jingzhao Ke,

Lishu Zheng

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(12), P. 2341 - 2341

Published: Dec. 20, 2024

Sexually propagated scleractinian corals are in high demand for coral reef restoration. However, threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck large-scale cultivation sexually reproducing corals. In this study, we analyzed proteomic signatures red eggs and spermatozoa from ecologically significant Galaxea fascicularis, using data-independent acquisition mass spectrometry (DIA-MS) method. A total 7741 7279 proteins mature were identified, respectively. Among these proteins, 596 spermatozoa-specific 1056 egg-specific. Additionally, 4413 differentially abundant (DAPs) among 3121 up-regulated 1292 spermatozoa. Furthermore, anenrichment analyses showed that DAPs identified mainly involved progesterone-mediated oocyte maturation pathway lectin pathway; detected insulin secretion metabolic pathways generation energy. This result will contribute discovery intrinsic regulation gamete fertilization. at least 57 associated with including fluorescent protein (RFP), vitellogenin (VG), egg (EP), testis-specific serine/threonine-protein kinase family (TSSKs), EF-hand Ca2+-binding (EFHC1 EFHC2). Particularly, third yolk EUPHY was reported first time G. fascicularis. conclusion, study unveiled groundbreaking insights into sexual reproduction, paving way more effective conservation sustainable development ecosystems

Language: Английский

Citations

0