It’s a matter of time: the role of transcriptional regulation in the circadian clock-pathogen crosstalk in plants DOI Open Access
María José de Leone, Carlos Esteban Hernando,

Santiago Mora-Garcı́a

et al.

Transcription, Journal Year: 2020, Volume and Issue: 11(3-4), P. 100 - 116

Published: Aug. 7, 2020

Most living organisms possess an internal timekeeping mechanism known as the circadian clock, which enhances fitness by synchronizing timing of biological processes with diurnal and seasonal environmental changes. In plants, pace these rhythms relies on oscillations in expression level hundreds genes tightly controlled a group core clock regulators co-regulators that engage transcriptional translational feedback loops. last decade, role several control defense responses has been addressed, growing amount evidence demonstrates regulation is relevant for plant immunity. A reciprocal connection between pathways was also established following observation Arabidopsis thaliana, well crop species like tomato, plant-pathogen interactions trigger reconfiguration network. this review, we summarize current knowledge regarding interaction biotic stress at level, discuss relevance crosstalk evolutionary arms race. better understanding could aid development genetic tools improve traditional breeding practices, enhancing tolerance to diseases threaten yield food security all around world.

Language: Английский

Arabidopsis thaliana GLYCINE RICH RNA‐BINDING PROTEIN 7 interaction with its iCLIP target LHCB1.1 correlates with changes in RNA stability and circadian oscillation DOI Creative Commons
Martin Lewinski,

Alexander Steffen,

Nitin Kachariya

et al.

The Plant Journal, Journal Year: 2023, Volume and Issue: 118(1), P. 203 - 224

Published: Dec. 20, 2023

SUMMARY The importance of RNA‐binding proteins (RBPs) for plant responses to environmental stimuli and development is well documented. Insights into the portfolio RNAs they recognize, however, clearly lack behind understanding gathered in non‐plant model organisms. Here, we characterize binding circadian clock‐regulated Arabidopsis thaliana GLYCINE‐RICH RNA‐BINDING PROTEIN 7 ( At GRP7) its target transcripts. We identified novel RNA targets from individual‐nucleotide resolution UV crosslinking immunoprecipitation (iCLIP) data using an improved bioinformatics pipeline that will be broadly applicable RBP iCLIP data. 2705 transcripts with sites were plants expressing GRP7‐GFP not recovered dead variant or GFP alone. A conserved motif enriched uridine residues was at GRP7 sites. NMR titrations confirmed preference a central U‐rich motif. Among bound RNAs, overrepresented. Peak abundance LHCB1.1 transcript encoding chlorophyll‐binding protein reduced overexpressing whereas it elevated atgrp7 mutants, indicating regulated by dose‐dependent manner. In GRP7, half‐life shorter compared wild‐type mutant plants, significantly longer. Thus, modulates oscillations vivo affecting stability.

Language: Английский

Citations

7
The circadian clock and thermal regulation in plants: novel insights into the role of positive circadian clock regulators in temperature responses DOI
María José de Leone, Marcelo J. Yanovsky

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(10), P. 2809 - 2818

Published: Feb. 19, 2024

Abstract The impact of rising global temperatures on crop yields is a serious concern, and the development heat-resistant varieties crucial for mitigating effects climate change agriculture. To achieve this, better understanding molecular basis thermal responses plants necessary. circadian clock plays central role in modulating plant biology synchrony with environmental changes, including temperature fluctuations. Recent studies have uncovered transcriptional activators core network responses. This expert view highlights key novel findings regarding RVE LNK gene families controlling expression patterns growth under different conditions, ranging from regular diurnal oscillations to extreme stress temperatures. These reinforce essential adaptation changing provide future improvement.

Language: Английский

Citations

2
PICLN modulates alternative splicing and light/temperature responses in plants DOI Creative Commons
Julieta L. Mateos, Sabrina E. Sanchez, Martina Legris

et al.

PLANT PHYSIOLOGY, Journal Year: 2022, Volume and Issue: 191(2), P. 1036 - 1051

Published: Nov. 24, 2022

Abstract Plants undergo transcriptome reprograming to adapt daily and seasonal fluctuations in light temperature conditions. While most efforts have focused on the role of master transcription factors, importance splicing factors modulating these processes is now emerging. Efficient pre-mRNA depends proper spliceosome assembly, which plants animals requires methylosome complex. Ion Chloride nucleotide-sensitive protein (PICLN) part complex both humans Arabidopsis (Arabidopsis thaliana), we show here that human PICLN ortholog rescues phenotypes picln mutants. Altered photomorphogenic photoperiodic responses mutants are associated with changes partially overlap those PROTEIN ARGININE METHYL TRANSFERASE5 (prmt5) Mammalian also acts concert Survival Motor Neuron (SMN) component GEMIN2 modulate late steps UsnRNP many alternative events regulated by but not PRMT5, main methylosome, controlled GEMIN2. As SM E1/PORCUPINE (SME1/PCP), low temperature, increases expression, aggravates morphological molecular defects Taken together, results establish a key for regulation mediating plant adaptation environmental

Language: Английский

Citations

11
A leaf-expressedTERMINAL FLOWER1ortholog from coffee with alternate splice forms alters flowering time and inflorescence branching inArabidopsis DOI Creative Commons
Carlos Henrique Cardon,

Victoria Lesy,

Catherine Fust

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 15, 2024

ABSTRACT Perennial, polycarpic species, such as Coffea sp L. (coffee), exhibit asynchronous flowering while maintaining concomitant vegetative growth. This growth dichotomy is associated directly with fruit development and maturation time. To identify molecular components that underlie flowering, we isolated phosphatidylethanolamine binding protein (PEBP) homologs expressed in coffee identified a gene high similarity to Arabidopsis TERMINAL FLOWER1-like . In Arabidopsis, interaction of TFL1 (AtTFL1) bZIP transcription factor floral regulator FD (AtFD) forms repressor complex maintains inflorescence meristems an indeterminate state. Unlike AtTFL1 , which only the shoot apical meristem, CaTFL1 transcript was detected exclusively leaves. Moreover, this retained intron, not reported for characterized through heterologous expression analysis. Ectopic overexpression transgenic plants caused extreme late or prevented flowering. However, most severe repressive activity occurred spliced out extra intron from Yeast two hybrid assay revealed encoded by mRNA interacts AtFD, well 14-3-3 protein. These findings suggest acts leaf-expressed repressor, whose controlled alternate splicing, may contribute coffee.

Language: Английский

Citations

2
It’s a matter of time: the role of transcriptional regulation in the circadian clock-pathogen crosstalk in plants DOI Open Access
María José de Leone, Carlos Esteban Hernando,

Santiago Mora-Garcı́a

et al.

Transcription, Journal Year: 2020, Volume and Issue: 11(3-4), P. 100 - 116

Published: Aug. 7, 2020

Most living organisms possess an internal timekeeping mechanism known as the circadian clock, which enhances fitness by synchronizing timing of biological processes with diurnal and seasonal environmental changes. In plants, pace these rhythms relies on oscillations in expression level hundreds genes tightly controlled a group core clock regulators co-regulators that engage transcriptional translational feedback loops. last decade, role several control defense responses has been addressed, growing amount evidence demonstrates regulation is relevant for plant immunity. A reciprocal connection between pathways was also established following observation Arabidopsis thaliana, well crop species like tomato, plant-pathogen interactions trigger reconfiguration network. this review, we summarize current knowledge regarding interaction biotic stress at level, discuss relevance crosstalk evolutionary arms race. better understanding could aid development genetic tools improve traditional breeding practices, enhancing tolerance to diseases threaten yield food security all around world.

Language: Английский

Citations

16