Amelioration of Chromium-Induced Oxidative Stress by Combined Treatment of Selected Plant-Growth-Promoting Rhizobacteria and Earthworms via Modulating the Expression of Genes Related to Reactive Oxygen Species Metabolism in Brassica juncea DOI Creative Commons
Pooja Sharma,

Rekha Chouhan,

Palak Bakshi

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: April 6, 2022

Chromium (Cr) toxicity leads to the enhanced production of reactive oxygen species (ROS), which are extremely toxic plant and must be minimized protect from oxidative stress. The potential plant-growth-promoting rhizobacteria (PGPR) earthworms in growth development has been extensively studied. present study was aimed at investigating effect two PGPR (Pseudomonas aeruginosa Burkholderia gladioli) along with (Eisenia fetida) on antioxidant defense system Brassica juncea seedlings under Cr reduced fresh dry weights seedlings, levels superoxide anion (O2•-), hydrogen peroxide (H2O2), malondialdehyde (MDA), electrolyte leakage (EL), lead membrane as well nuclear damage cellular viability B. seedlings. activities enzymes, viz., dismutase (SOD), guaiacol peroxidase (POD), ascorbate (APOX), glutathione (GPOX), dehydroascorbate reductase (DHAR), (GR) were increased; however, a reduction observed activity catalase (CAT) Inoculation addition all other enzymes except GPOX, observed. For total lipid- water-soluble antioxidants non-enzymatic antioxidants, ascorbic acid glutathione, an enhance accumulation upon inoculation earthworms. supplementation (combined treatment) both (ROS) MDA content by modulating plant. histochemical studies also corroborated that combined application O2•-, H2O2, lipid peroxidation, improved cell viability. expression key enzyme genes, SOD, CAT, POD, APOX, GR, DHAR, GST showed upregulation these genes post-transcriptional level treatment earthworms, thereby corresponding biomass. However, RBOH1 gene noticed supplemented grown results provided sufficient evidence regarding role amelioration Cr-induced stress juncea.

Language: Английский

Temperature changes in the root ecosystem affect plant functionality DOI Creative Commons
Mary Paz González‐García, Carlos M. Conesa, Alberto Lozano-Enguita

et al.

Plant Communications, Journal Year: 2022, Volume and Issue: 4(3), P. 100514 - 100514

Published: Dec. 30, 2022

Climate change is increasing the frequency of extreme heat events that aggravate its negative impact on plant development and agricultural yield. Most experiments designed to study adaption stress apply homogeneous high temperatures both shoot root. However, this treatment does not mimic conditions in natural fields, where roots grow a dark environment with descending temperature gradient. Excessively severely decrease cell division root meristem, compromising growth, while quiescent center cells, likely an attempt maintain stem niche under such harsh conditions. Here, we engineered TGRooZ, device generates gradient for vitro or greenhouse growth assays. The systems plants exposed but cultivated TGRooZ efficiently their functionality sustain proper development. Furthermore, gene expression rhizosphere microbiome composition are significantly less affected TGRooZ-grown than high-temperature-grown roots, correlating higher functionality. Our data indicate use heat-stress studies can improve our knowledge response temperatures, demonstrating applicability from laboratory field.

Language: Английский

Citations

60
Comparative Genome Analysis Reveals Phylogenetic Identity of Bacillus velezensis HNA3 and Genomic Insights into Its Plant Growth Promotion and Biocontrol Effects DOI
Doaa S. Zaid,

Shuyun Cai,

Chang Hu

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(1)

Published: Feb. 2, 2022

This study is the primary initiative to identify Bacillus velezensis HNA3 whole genome sequence and reveal its genomic properties as an effective biocontrol agent against plant pathogens a growth stimulator. genetic profile can be used reference for future studies that applied highly biofertilizer bio fungicide inoculum improve agriculture productivity.

Language: Английский

Citations

57
Plant-Microbiota Interactions in Abiotic Stress Environments DOI Creative Commons

Natsuki Omae,

Kenichi Tsuda

Molecular Plant-Microbe Interactions, Journal Year: 2022, Volume and Issue: 35(7), P. 511 - 526

Published: March 25, 2022

Abiotic stress adversely affects cellular homeostasis and ultimately impairs plant growth, posing a serious threat to agriculture. Climate change modeling predicts increasing occurrences of abiotic stresses such as drought extreme temperature, resulting in decreasing the yields major crops rice, wheat, maize, which endangers food security for human populations. Plants are associated with diverse taxonomically structured microbial communities that called microbiota. Plant microbiota often assist growth tolerance by providing water nutrients plants modulating metabolism physiology and, thus, offer potential increase crop production under stress. In this review, we summarize recent progress on how plants, microbiota, plant-microbe interactions, microbe-microbe microbes affect conditions, focus drought, salt, temperature We also discuss important steps utilize agriculture stress.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed CC BY-NC-ND 4.0 International license.

Language: Английский

Citations

56
Identification of heavy metals tolerant Brevundimonas sp. from rhizospheric zone of Saccharum munja L. and their efficacy in in-situ phytoremediation DOI
Pooja Sharma, Preeti Chaturvedi, Ram Chandra

et al.

Chemosphere, Journal Year: 2022, Volume and Issue: 295, P. 133823 - 133823

Published: Feb. 1, 2022

Language: Английский

Citations

54
Amelioration of Chromium-Induced Oxidative Stress by Combined Treatment of Selected Plant-Growth-Promoting Rhizobacteria and Earthworms via Modulating the Expression of Genes Related to Reactive Oxygen Species Metabolism in Brassica juncea DOI Creative Commons
Pooja Sharma,

Rekha Chouhan,

Palak Bakshi

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: April 6, 2022

Chromium (Cr) toxicity leads to the enhanced production of reactive oxygen species (ROS), which are extremely toxic plant and must be minimized protect from oxidative stress. The potential plant-growth-promoting rhizobacteria (PGPR) earthworms in growth development has been extensively studied. present study was aimed at investigating effect two PGPR (Pseudomonas aeruginosa Burkholderia gladioli) along with (Eisenia fetida) on antioxidant defense system Brassica juncea seedlings under Cr reduced fresh dry weights seedlings, levels superoxide anion (O2•-), hydrogen peroxide (H2O2), malondialdehyde (MDA), electrolyte leakage (EL), lead membrane as well nuclear damage cellular viability B. seedlings. activities enzymes, viz., dismutase (SOD), guaiacol peroxidase (POD), ascorbate (APOX), glutathione (GPOX), dehydroascorbate reductase (DHAR), (GR) were increased; however, a reduction observed activity catalase (CAT) Inoculation addition all other enzymes except GPOX, observed. For total lipid- water-soluble antioxidants non-enzymatic antioxidants, ascorbic acid glutathione, an enhance accumulation upon inoculation earthworms. supplementation (combined treatment) both (ROS) MDA content by modulating plant. histochemical studies also corroborated that combined application O2•-, H2O2, lipid peroxidation, improved cell viability. expression key enzyme genes, SOD, CAT, POD, APOX, GR, DHAR, GST showed upregulation these genes post-transcriptional level treatment earthworms, thereby corresponding biomass. However, RBOH1 gene noticed supplemented grown results provided sufficient evidence regarding role amelioration Cr-induced stress juncea.

Language: Английский

Citations

53