Phylogenetic Analyses and Transcriptional Survey Reveal the Characteristics, Evolution, and Expression Profile of NBS-Type Resistance Genes in Papaya

Agronomy, Journal Year: 2023, Volume and Issue: 13(4), P. 970 - 970

Published: March 25, 2023

Carica papaya maintains an abnormally small but complete NLR family while showing weak disease resistance. To better understand their origin, evolution, and biological function, we identified 59 genes via a customized RGAugury investigated characteristics, evolutionary history, expression profiles based on the improved genome large-scale RNA-seq data. The results indicated that duplication is major force driving formation of family. Synteny analyses other angiosperms showed both insertion inheritance-derived NLRs are present in papaya. Transcriptome-based network revealed actively involved biotic stress responses. For example, papaya-specific inserted TNL was up-regulated strongly by fungal infection. Both transcriptome qRT-PCR confirmed divergence RNL RCNL, pair tandem different co-expression modules. Furthermore, observed gene cluster composed five duplicated CNLs, dosage effects functional differentiation disease-resistance during evolution. This research will enhance our knowledge special papaya, which may serve as model plant for genetic studies.

Language: Английский

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The First Genome-Wide Mildew Locus O Genes Characterization in the Lamiaceae Plant Family

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13627 - 13627

Published: Sept. 4, 2023

Powdery mildew (PM) is a widespread plant disease that causes significant economic losses in thousands crops of temperate climates, including Lamiaceae species. Multiple scientific studies describe peculiar form PM-resistance associated at the inactivation specific members Mildew Locus O (MLO) gene family, referred to as mlo-resistance. The characterization MLO genes, genomic level, would be first step toward their potential use breeding programs. We carried out genome-wide family 11 species, providing manual curated catalog 324 proteins. Evolutionary history and phylogenetic relationships were studied through maximum likelihood analysis motif patter reconstruction. Our approach highlighted seven different clades diversified starting from an ancestral domain pattern organized 18 highly conserved motifs. In addition, 74 putative PM susceptibility clustering clade V, identified. Finally, we performed codon-based evolutionary analysis, revealing general high level purifying selection eleven families, occurrence few regions under diversifying candidate factors. results this work may help address further biological questions concerning MLOs involved susceptibility. follow-up studies, it could investigated whether silencing or loss-of-function mutations one more these genes lead resistance.

Language: Английский

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NB-LRR Lineage-Specific Equipment Is Sorted Out by Sequence Pattern Adaptation and Domain Segment Shuffling

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 14269 - 14269

Published: Nov. 17, 2022

The nucleotide-binding and leucine-rich repeat (NB-LRR) genes, also known as resistance (R)-genes, play an important role in the activation of immune responses. In recent years, large-scale studies have been performed to highlight diversification plant NB-LRR repertories. It is well that, provide new functionalities, sequences are subject duplication, domain fusions acquisition other kinds mutations. Although some mechanisms that govern protein adaptations uncovered, retrace plant-lineage-specific evolution routes R structure, a multi-genome comparative analysis was performed. This study allowed us define groups genes sharing homology relationships across different species. worth noting most populated contained well-characterized proteins. arsenal profile such investigated five botanical families, including crop species, underline specific adaptation signatures. addition, dissection 70 NB domains R-genes revealed core motifs from which three main classes diversified. structural remodeling segments shaped repertoires observed each provided evolutionary functional insights on shuffling. Taken together, findings improved our understanding molecular adaptive selection occurring at loci.

Language: Английский

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