Comprehensive LC-MS/MS analysis of nitrogen-related plant metabolites
Journal of Experimental Botany,
Journal Year:
2024,
Volume and Issue:
75(17), P. 5390 - 5411
Published: March 25, 2024
Abstract
We
have
developed
and
validated
a
novel
LC-MS/MS
method
for
simultaneously
analyzing
amino
acids,
biogenic
amines,
their
acetylated
methylated
derivatives
in
plants.
This
involves
one-step
extraction
of
2–5
mg
lyophilized
plant
material
followed
by
fractionation
different
amine
forms,
exploits
an
efficient
combination
hydrophilic
interaction
liquid
chromatography
(HILIC),
reversed
phase
(RP)
with
pre-column
derivatization,
tandem
mass
spectrometry
(MS).
approach
enables
high-throughput
processing
samples,
significantly
reducing
the
time
needed
analysis
its
cost.
also
present
new
synthetic
route
deuterium-labeled
polyamines.
The
was
rigorously
quantifying
levels
nitrogen-related
metabolites
seedlings
seven
species,
including
Arabidopsis,
maize,
barley,
all
which
are
commonly
used
model
organisms
science
research.
Our
results
revealed
substantial
variations
abundance
these
between
developmental
stages,
growth
conditions,
particularly
various
polyamine
fractions.
However,
biological
relevance
is
currently
unclear.
Overall,
this
work
contributes
to
providing
powerful
analytical
tool
setting
stage
future
investigations
into
functions
Language: Английский
Nucleotides and nucleotide derivatives as signal molecules in plants
Journal of Experimental Botany,
Journal Year:
2024,
Volume and Issue:
75(22), P. 6918 - 6938
Published: Sept. 9, 2024
Abstract
In
reaction
to
a
stimulus,
signaling
molecules
are
made,
generate
response,
and
then
degraded.
Nucleotides
classically
associated
with
central
metabolism
nucleic
acid
biosynthesis,
but
there
number
of
nucleotides
nucleotide
derivatives
in
plants
which
this
simple
definition
molecule
applies
whole
or
at
least
part.
These
include
cytokinins
chloroplast
guanosine
tetraposphate
(ppGpp),
as
well
extracellular
canonical
such
ATP
(eATP)
NAD+
(eNAD+).
addition,
is
series
compounds
derived
from
ADP
ribose
(ADPR),
ATP–ADPR
dinucleotides
their
hydrolysis
products
(e.g.
pRib-AMP)
together
different
variants
cyclic
ADPR
(cADPR,
2´-cADPR,
3´-cADPR),
also
3´,5´-cAMP
2´,3´-cyclic
nucleoside
monophosphates.
Interestingly,
some
these
have
recently
been
shown
play
role
pathogen
defense.
review,
we
highlight
exciting
new
developments.
We
review
that
considered
candidates
for
molecules,
example
purine
deoxynucleosides,
discuss
more
controversial
cases.
Language: Английский
Phenotypic Traits, Hormonal Distribution, and Metabolite Profiling of Isatis indigotica Seeds from 21 Samples in China: A Traditional Chinese Medicinal Herb
Lunyan Wang,
No information about this author
Jia Liu,
No information about this author
Yilun Dong
No information about this author
et al.
Plants,
Journal Year:
2025,
Volume and Issue:
14(7), P. 1096 - 1096
Published: April 1, 2025
"Banlangen"
(Isatis
indigotica
Fort.,
indigowoad
root)
is
the
dried
root
of
a
plant
in
Cruciferae
(Brassicaceae)
family,
which
has
been
cultivated
China
for
over
2000
years.
This
herb
significant
medicinal
value
and
played
an
important
therapeutic
role
during
COVID-19
pandemic.
Isatis
widely
China,
with
varying
seed
quality
across
different
regions.
Investigating
distribution
endogenous
metabolites
from
various
regions
great
significance
its
efficient
utilization.
In
this
study,
21
samples
seeds
were
collected
15
analyzed
using
gas
chromatography-mass
spectrometry
(GC-MS)
liquid
(LC-MS).
A
systematic
analysis
was
conducted
on
phenotypes,
germination
rate,
metabolites,
hormones.
The
results
showed
that
germplasm
traits
northern
generally
superior
to
those
southern
size
positively
correlated
accumulation
sugars
(D-allose,
D-(+)-cellobiose,
maltitol,
α-D-glucopyranoside,
d-galactose,
maltose,
D-(-)-fructose,
galactitol)
organic
acids
(hexadecanoic
acid,
benzoic
propanedioic
butanedioic
cinnamic
acid).
Additionally,
SuQian
Shanxi
closely
related
hormone
(gibberellic
acid
abscisic
study
provides
valuable
data
support
evaluation
sources
plants
implications
cultivation
identification
varieties.
Language: Английский
Bacillus licheniformis Jrh14‐10 enhances alkaline tolerance in Arabidopsis thaliana by regulating crosstalk between ethylene and polyamine pathways
Physiologia Plantarum,
Journal Year:
2024,
Volume and Issue:
176(4)
Published: July 1, 2024
Abstract
Plant
growth‐promoting
rhizobacteria
(PGPR)
are
known
for
their
role
in
ameliorating
plant
stress,
including
alkaline
yet
the
mechanisms
involved
not
fully
understood.
This
study
investigates
impact
of
various
inoculum
doses
Bacillus
licheniformis
Jrh14‐10
on
Arabidopsis
growth
under
stress
and
explores
underlying
tolerance
enhancement.
We
found
that
all
tested
improved
NaHCO
3
‐treated
seedlings,
with
10
9
cfu/mL
being
most
effective.
Transcriptome
analysis
indicated
downregulation
ethylene‐related
genes
an
upregulation
polyamine
biosynthesis
following
treatment
conditions.
Further
qRT‐PCR
confirmed
suppression
ethylene
signaling
genes,
alongside
activation
‐stressed
seedlings
treated
Jrh14‐10.
Genetic
showed
signaling‐deficient
mutants
(
etr1‐3
ein3‐1
)
exhibited
greater
to
than
wild
type,
effect
was
significantly
diminished
these
mutants.
Additionally,
unable
produce
1‐aminocyclopropane‐1‐carboxylic
acid
(ACC)
deaminase,
indicating
it
does
reduce
precursor
ACC
.
However,
increased
levels
polyamines
(putrescine,
spermidine,
spermine)
stressed
spermidine
particularly
effective
reducing
H
2
O
enhancing
F
v
/F
m
stress.
These
findings
reveal
a
novel
mechanism
PGPR‐induced
tolerance,
highlighting
crosstalk
between
pathways,
suggest
strategic
redirection
S‐adenosylmethionine
towards
combat
Language: Английский