Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress DOI Open Access
Chao Zhao, Hao Bai, Chaoshuo Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9103 - 9103

Published: Aug. 22, 2024

DNA-binding one zinc finger (DOF) transcription factors are crucial plant-specific regulators involved in growth, development, signal transduction, and abiotic stress response generation. However, the genome-wide identification characterization of AcDOF genes their regulatory elements kiwifruit (Actinidia chinensis) has not been thoroughly investigated. In this study, we screened genome database identified 42 (AcDOF1 to AcDOF42). Phylogenetic analysis facilitated categorization these into five subfamilies (DOF-a, DOF-b, DOF-c, DOF-d, DOF-e). We further analyzed motifs, conserved domains, gene structures, collinearity AcDOFgene family. Gene ontology (GO) enrichment indicated significant “flower development” term “response stress” category. Promoter prediction revealed numerous cis-regulatory related responses light, hormones, low-temperature drought promoters. RNA-seq expression profiles demonstrated tissue-specific genes. Quantitative real-time PCR results showed that six selected (AcDOF04, AcDOF09, AcDOF11, AcDOF13, AcDOF21, AcDOF22) were differentially induced by abscisic acid (ABA), methyl jasmonate (MeJA), cold, salt, stresses, with AcDOF22 specifically expressed at high levels drought-tolerant cultivars. Further experiments transient overexpression leaf disks reduced water loss chlorophyll degradation. Additionally, was localized nucleus exhibited transcriptional activation, enhancing resistance activating downstream marker AcDREB2A. These findings lay foundation for elucidating molecular mechanisms offer new insights drought-resistant breeding.

Language: Английский

Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress DOI Open Access
Chao Zhao, Hao Bai, Chaoshuo Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9103 - 9103

Published: Aug. 22, 2024

DNA-binding one zinc finger (DOF) transcription factors are crucial plant-specific regulators involved in growth, development, signal transduction, and abiotic stress response generation. However, the genome-wide identification characterization of AcDOF genes their regulatory elements kiwifruit (Actinidia chinensis) has not been thoroughly investigated. In this study, we screened genome database identified 42 (AcDOF1 to AcDOF42). Phylogenetic analysis facilitated categorization these into five subfamilies (DOF-a, DOF-b, DOF-c, DOF-d, DOF-e). We further analyzed motifs, conserved domains, gene structures, collinearity AcDOFgene family. Gene ontology (GO) enrichment indicated significant “flower development” term “response stress” category. Promoter prediction revealed numerous cis-regulatory related responses light, hormones, low-temperature drought promoters. RNA-seq expression profiles demonstrated tissue-specific genes. Quantitative real-time PCR results showed that six selected (AcDOF04, AcDOF09, AcDOF11, AcDOF13, AcDOF21, AcDOF22) were differentially induced by abscisic acid (ABA), methyl jasmonate (MeJA), cold, salt, stresses, with AcDOF22 specifically expressed at high levels drought-tolerant cultivars. Further experiments transient overexpression leaf disks reduced water loss chlorophyll degradation. Additionally, was localized nucleus exhibited transcriptional activation, enhancing resistance activating downstream marker AcDREB2A. These findings lay foundation for elucidating molecular mechanisms offer new insights drought-resistant breeding.

Language: Английский

Citations

3