Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 340, P. 113931 - 113931
Published: Dec. 28, 2024
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 308, P. 142626 - 142626
Published: April 2, 2025
Language: Английский
Citations
0
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 21, 2025
Drought stress could suppress the carbon assimilation and limit nutrient uptake of pear plants, thus affecting their growth severely impacting quality yield fruit. ABA is a hormone reported to alleviate drought in numerous plants. However, whether how functions responses plants yet explored. Here, address this gap, seedlings (Pyrus betulaefolia) were used subjected PEG-induced conditions with or without additional various doses. The results showed that while caused severe leaf water loss damage, applying at 50 µM 100 dramatically amended phenomenon, as indicated by markedly increased relative content, notably decreased electrolyte leakage malondialdehyde content. Based on RNA sequencing related physiological indices, it was found grossly disrupted chlorophyll synthesis photosynthesis. It induced over-production reactive oxygen species (ROS) broke ROS homeostasis, despite pronounced increases biosynthesis/content signaling, flavonoid synthesis, antioxidant enzyme activities, well sugar metabolism. applications significantly elevated expressions genes photosynthesis, partially boosting SPAD Fv/Fm values. In addition, treatments further prominently accelerate processes ABA, flavonoids, enzymes up-regulating corresponding genes, resulting endogenous accumulation enzymatic activity improvement, thereby expediting scavenging. Of course, metabolism pathway also outstandingly enhanced balance response seedlings. Moreover, through WGCNA analysis, core turquoise module associated ABA-attenuated identified, portion key transcription factors (TFs) some hub characterized, particularly for ERF, WRKY, MYB, bHLH, NAC TFs, CSP, COR, DHN genes. Overall, our study reveals exogenous help efficiently scavenge drought-induced improving photosynthesis capacity, accumulation, catabolism, system, etc. These will provide scientific basis practical direction utilizing mitigate adverse effects starvation from persistent high temperature summer. application ameliorated damage seedlings, working routes are reinforcement photosystem, biosynthesis metabolism, activities relevant regulatory network complex, primarily concerned focus potential target named DHN.
Language: Английский
Citations
0
The Plant Journal, Journal Year: 2025, Volume and Issue: 122(2)
Published: April 1, 2025
SUMMARY With the ongoing rise in global temperatures, drought stress has become a significant threat to normal growth and development of horticultural crops. Identifying regulatory genes is key genetic improvement. Extensive research highlighted pivotal role WRKY transcription factors orchestrating plant responses both biotic abiotic stresses. However, their precise involvement tolerance related molecular mechanisms have yet be fully elucidated. In this study, we demonstrated that MdWRKY71 functioned as positive regulator apple. Overexpressing apple improved tolerance, while silencing it had opposite effect. Additionally, under stress, compared with control, chlorophyll fluorescence values, superoxide dismutase (SOD), peroxidase levels were elevated ‐overexpressing tobacco transgenic materials. Interaction analysis showed directly binds W‐box element MdFeSOD promoter activates its transcription. We used yeast two‐hybrid screening identify potential interactors confirmed interaction between MdARF3 using Pull‐down, bimolecular complementation, luciferase complementation imaging assays. Interestingly, enhanced MdWRKY71‐mediated transcriptional activation through interaction. summary, our findings revealed MdWRKY71–MdARF3 module synergistically upregulates expression SOD enzyme activity response stress. This uncovers new mechanism presents feasible strategy enhance stabilizing biosynthesis dismutase.
Language: Английский
Citations
0
Horticulture Research, Journal Year: 2025, Volume and Issue: 12(7)
Published: April 30, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9103 - 9103
Published: Aug. 22, 2024
DNA-binding one zinc finger (DOF) transcription factors are crucial plant-specific regulators involved in growth, development, signal transduction, and abiotic stress response generation. However, the genome-wide identification characterization of AcDOF genes their regulatory elements kiwifruit (Actinidia chinensis) has not been thoroughly investigated. In this study, we screened genome database identified 42 (AcDOF1 to AcDOF42). Phylogenetic analysis facilitated categorization these into five subfamilies (DOF-a, DOF-b, DOF-c, DOF-d, DOF-e). We further analyzed motifs, conserved domains, gene structures, collinearity AcDOFgene family. Gene ontology (GO) enrichment indicated significant “flower development” term “response stress” category. Promoter prediction revealed numerous cis-regulatory related responses light, hormones, low-temperature drought promoters. RNA-seq expression profiles demonstrated tissue-specific genes. Quantitative real-time PCR results showed that six selected (AcDOF04, AcDOF09, AcDOF11, AcDOF13, AcDOF21, AcDOF22) were differentially induced by abscisic acid (ABA), methyl jasmonate (MeJA), cold, salt, stresses, with AcDOF22 specifically expressed at high levels drought-tolerant cultivars. Further experiments transient overexpression leaf disks reduced water loss chlorophyll degradation. Additionally, was localized nucleus exhibited transcriptional activation, enhancing resistance activating downstream marker AcDREB2A. These findings lay foundation for elucidating molecular mechanisms offer new insights drought-resistant breeding.
Language: Английский
Citations
3
Agriculture, Journal Year: 2024, Volume and Issue: 14(11), P. 2047 - 2047
Published: Nov. 14, 2024
Rubus idaeus is one of the primary cultivated species raspberries, renowned for its appealing color, distinctive flavor and numerous health benefits. WIP proteins, which contain three conserved amino acids (W: Tryptophan, I: Isoleucine, P: Proline) four zinc finger motifs in a highly C-terminal region, are members A1d subgroup C2H2 proteins. Drought main limiting factors plant growth development, restricts cultivation utilization raspberry northwest China. In this study, to obtain candidate genes drought resistance, we identified key related genes, RiWIPs, from R. analyzed their bioinformation tissue stress response expression drought. We found that there RiWIPs they located on chromosomes 3, 4 6 idaeus, respectively. The open reading frames (ORFs) ranged 870 1056 bp length, encoding 289 372 acid residues. proteins were feature diverse motifs. promoters contained abundant cis-elements growth, development response. Tissue-specific analysis revealed expressed leaves, stems roots both drought-susceptible drought-tolerant cultivars, except RiWIP2, was only one. Under stress, transcriptional activity increased different degrees with specificity roots. Our study demonstrated role provided marker gene, resistance subsequent drought-resistant breeding idaeus.
Language: Английский
Citations
1
Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 219, P. 109399 - 109399
Published: Dec. 9, 2024
Language: Английский
Citations
1
Gene, Journal Year: 2024, Volume and Issue: 938, P. 149176 - 149176
Published: Dec. 16, 2024
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 10952 - 10952
Published: Oct. 11, 2024
Heavy metals in soil can inflict direct damage on plants growing within it, adversely affecting their growth height, root development, leaf area, and other physiological traits. To counteract the toxic impacts of heavy plant mitigate metal stress through mechanisms such as chelation, vacuolar compartmentalization, regulation transporters, enhancement antioxidant functions. WRKY transcription factors (TFs) play a crucial role development well responses to both biotic abiotic stresses; notably, is classified an stressor. An increasing number studies have highlighted significant proteins regulating across various levels. Upon entry ions into cells, production reactive oxygen species (ROS) triggered, leading phosphorylation activation TFs MAPK cascade signaling. Activated then modulate processes by upregulating or downregulating expression downstream genes confer tolerance plants. This review provides overview research advancements regarding ion stress—including cadmium (Cd), arsenic (As), copper (Cu)—and aluminum (Al) toxicity.
Language: Английский
Citations
1
Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 340, P. 113931 - 113931
Published: Dec. 28, 2024
Language: Английский
Citations
0