Transcriptional Analysis Reveals the Differences in Response of Floral Buds to Boron Deficiency Between Two Contrasting Brassica napus Varieties

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 859 - 859

Published: March 10, 2025

Boron (B) is an essential micronutrient for the development of crops, and its reproductive stage particularly sensitive to B deficiency. Brassica napus L., as important oil-crop species, extremely vulnerable The typical B-deficient symptom “flowering without seed setting” usually results in severe yield loss. However, few studies have focused on response organs In this study, B-efficient variety “Zhongshuang 11” (ZS11) B-inefficient “Westar 10” (W10) were selected be cultivated at developmental (BBCH15) a pot experiment, both with supply. Clear phenotype differences deficiency between two varieties’ flowers appeared only stage, W10 showed symptoms delayed flower opening, stigma exsertion, resulted abortion. Transcriptome analysis early buds varieties supply (+B) free (−B) treatments revealed that had more differentially expressed genes (DEGs) corresponding greater susceptibility −B. As potential mechanisms improve utilization, we analyzing expression profiles transporter-related phytohormone metabolism-related genes. BnaC05.NIP7;1, BnaC08.NIP3;1, BnaBOR2s identified key which could enhance capacity translocation ZS11. Additionally, combined concentration measurement, significant increase IAA drastic decrease JA predominantly lead abnormal W10’s buds. BnaC02.NIT2 (Nitrilase 2) BnaKAT5s (3-Ketoacyl-CoA Thiolase 5), are biosynthesis genes, respectively, responsible changes concentrations under These candidate may regulate genotype rapeseed −B different varieties. It also has breed utilization would condition.

Language: Английский

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Identification of elite alleles associated with glume-opening angle in rice natural population

Euphytica, Journal Year: 2025, Volume and Issue: 221(4)

Published: March 20, 2025

Language: Английский

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The F box protein INHIBITOR FOR BROWN FURROWS 1 (IBF1) regulates flavonoid accumulation in rice hull by promoting degradation of chalcone synthase 1

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Flavonoids are secondary metabolites of plants that play various roles in plants. The transcriptional level regulation flavonoid synthesis has been extensively studied, but research on the protein is still limited. In present study, a brown hull mutant, bh2 , was screened from an ethane methyl sulfonate (EMS)‐induced bank seeds indica cultivar RH2B. mutant exhibited phenotype and higher levels total flavonoids anthocyanins compared with wild‐type We identified gene INHIBITOR FOR BROWN FURROWS 1 ( IBF1 ) through MutMap analysis subsequently cloned it. encodes F‐box involved formation SCF (S‐phase kinase‐associated [SKP1], Cullin, F‐box) complex Oryza sativa SKP1‐like proteins OSK1/OSK20. Through yeast two‐hybrid, bimolecular fluorescence complementation, pull‐down assays, interaction chalcone synthase (CHS1) confirmed. This facilitated degradation CHS1 ubiquitin‐26S proteasome system. ibf1 chs1 double mutants normal color, restoring . Genetic suggested regulates color CHS1‐dependent manner. Collectively, our study suggests serves as crucial negative regulator controls biosynthesis by mediating degradation, thereby regulating color.

Language: Английский

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PpERF17 alleviates peach fruit postharvest chilling injury under elevated CO2 by activating jasmonic acid and γ-aminobutyric acid biosynthesis

Horticulture Research, Journal Year: 2025, Volume and Issue: 12(4)

Published: Jan. 15, 2025

Abstract Internal browning (IB) is a common chilling injury (CI) feature in peach fruit after prolonged cold storage. Our previous study demonstrated that low O2 and elevated CO2 (eCO2) condition of modified atmosphere (MA) storage alleviated CI by facilitating the accumulation jasmonic acids (JAs) γ-aminobutyric acid (GABA) ‘Zhonghuashoutao’ (‘ZHST’) fruit. Here we show 10% alone can improve tolerance, with ethylene response factor 17 (PpERF17) identified as pivotal transcription (TF) promotes biosynthesis JAs GABA. Stable transformation PpERF17 tobacco resulted reduced damage, attributed to decreased levels hydrogen peroxide (H2O2) malondialdehyde (MDA), well enhanced Moreover, under eCO2, PpMYC2.1, master regulator JA signaling, was found activate 13S-lipoxygenase (Pp13S-LOX), allene oxide synthase (PpAOS), 12-oxophytodienoate reductase 3 (PpOPR3), glutamate decarboxylase (PpGAD), while also inducing expression upstream TF PpERF17, thereby establishing positive feedback loops upregulating GABA biosynthesis. Finally, application methyl jasmonate (MeJA) before shelf transfer from development, due increased result raised related biosynthetic genes. Collectively, our results suggest eCO2-induced enhances activating signaling pathway. This contributes loop mediated ultimately alleviating through sustained

Language: Английский

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Timing is everything: OsMYC2 controls diurnal flower‐opening time in rice

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(6), P. 2583 - 2584

Published: Sept. 1, 2024

Language: Английский

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Revealing Physiological Basis for Floret Opening Difference Between Indica and Japonica Rice: Based on Floral Structure, Transcriptome, and Endogenous Floret Opening Regulator

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1396 - 1396

Published: Oct. 30, 2024

The differing floret opening times between subsp.

Language: Английский

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OsMYB1 antagonizes OsSPL14 to mediate rice resistance to brown planthopper and Xanthomonas oryzae pv. oryzae

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

In their natural habitats, plants are concurrently attacked by different biotic factors. Xanthomonas oryzae pv. oryzae (Xoo) is a pathogen that severely deteriorates rice yield and quality, brown planthopper (BPH; Nilaparvata lugens) specific insect pest with the damage topping other pathogens. Although genes for respective resistance to BPH Xoo have been widely reported, few studies pay attention simultaneous both. this study, we identified MYB transcription factor, OsMYB1, which exhibited diverse transcriptional regulatory capabilities negative role in both Xoo. Biochemical genetic analysis proved OsMYB1 be TF could interact OsSPL14, positive regulator of OsSPL14 mutants showed increased sensitivity BPH, suggesting contrary to OsMYB1in regulating these two stresses. Consistently, OsSPL14 displayed opposite functions defense-related genes. can form regulation complexes repressor OsJAZs instead co-repressor TOPLESS possibly realize its repression function. Taken together, concluded interacting TFs rice, played antagonistic roles

Language: Английский

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OsMYB1 antagonizes OsSPL14 to mediate rice resistance to brown planthopper and Xanthomonas oryzae pv. oryzae

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 44(1)

Published: Dec. 26, 2024

Language: Английский

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