OsERF2 Acts as a Direct Downstream Target of OsEIL1 to Negatively Regulate Salt Tolerance in Rice DOI Creative Commons
Jiahao Zhou, Shengliang Fang, Xinjie Liu

et al.

Rice, Journal Year: 2025, Volume and Issue: 18(1)

Published: April 24, 2025

Salinity is a significant limiting factor that adversely affects plant growth, distribution and crop yield. Ethylene responsive factors play crucial roles in responses to tolerance of various abiotic stresses. Recently, we revealed OsERF2 involved root growth by transcriptionally regulating hormone sugar signaling rice. Here, report direct target gene OsEIL1 negatively regulates salt Compared the wild type, gain-of-function mutant (nsf2857) knockdown via an artificial microRNA (Ami-ERF2) exhibited decreased increased tolerance, respectively. The enhanced observed Ami-OsERF2 lines was associated with lower accumulations malondialdehyde reactive oxygen species (ROS) under stress, while opposite true for nsf2857 plants, which tolerance. At transcriptional level, several stress-related genes encoding ROS NAD(P)H-related oxidoreductases were downregulated plants but upregulated Ami-ERF2 plants. Furthermore, yeast one-hybrid ChIP assays can bind EBS cis element present promoter (-bp), suggesting may directly regulate expression OsERF2. Collectively, our findings indicate downstream regulation rice, highlighting its potential application genetic improvement stresses this crop.

Language: Английский

OsERF2 Acts as a Direct Downstream Target of OsEIL1 to Negatively Regulate Salt Tolerance in Rice DOI Creative Commons
Jiahao Zhou, Shengliang Fang, Xinjie Liu

et al.

Rice, Journal Year: 2025, Volume and Issue: 18(1)

Published: April 24, 2025

Salinity is a significant limiting factor that adversely affects plant growth, distribution and crop yield. Ethylene responsive factors play crucial roles in responses to tolerance of various abiotic stresses. Recently, we revealed OsERF2 involved root growth by transcriptionally regulating hormone sugar signaling rice. Here, report direct target gene OsEIL1 negatively regulates salt Compared the wild type, gain-of-function mutant (nsf2857) knockdown via an artificial microRNA (Ami-ERF2) exhibited decreased increased tolerance, respectively. The enhanced observed Ami-OsERF2 lines was associated with lower accumulations malondialdehyde reactive oxygen species (ROS) under stress, while opposite true for nsf2857 plants, which tolerance. At transcriptional level, several stress-related genes encoding ROS NAD(P)H-related oxidoreductases were downregulated plants but upregulated Ami-ERF2 plants. Furthermore, yeast one-hybrid ChIP assays can bind EBS cis element present promoter (-bp), suggesting may directly regulate expression OsERF2. Collectively, our findings indicate downstream regulation rice, highlighting its potential application genetic improvement stresses this crop.

Language: Английский

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