RNAi and genome editing of sugarcane: Progress and prospects

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

Sugarcane, which provides 80% of global table sugar and 40% biofuel, presents unique breeding challenges due to its highly polyploid, heterozygous, frequently aneuploid genome. Significant progress has been made in developing genetic resources, including the recently completed reference genome sugarcane cultivar R570 pan-genomic resources from sorghum, a closely related diploid species. Biotechnological approaches RNA interference (RNAi), overexpression transgenes, gene editing technologies offer promising avenues for accelerating improvement. These methods have successfully targeted genes involved important traits such as sucrose accumulation, lignin biosynthesis, biomass oil stress response. One main transformation methods-biolistic transfer or Agrobacterium-mediated transformation-coupled with efficient tissue culture protocols, is typically used implementing these biotechnology approaches. Emerging show promise overcoming current limitations. The use morphogenic can help address genotype constraints improve efficiency. Tissue culture-free technologies, spray-induced silencing, virus-induced editing, potential functional genomics studies. Additionally, novel base prime orthogonal synthetic transcription factors, directed evolution present opportunities enhancing traits. advances collectively aim sugarcane's efficiency crop both biofuel production. This review aims discuss methodologies, focus on RNAi approaches, how be inform targets, future improvements applications.

Language: Английский

Attaining the Promise of Geminivirus-Based Vectors in Plant Genome Editing

Viruses, Journal Year: 2025, Volume and Issue: 17(5), P. 631 - 631

Published: April 27, 2025

Over the last 40 years, several studies have provided evidence demonstrating that viral vectors can result in effective gene targeting/insertions a host’s genome. The traditional approaches of knock-down, -out, or -in involve an intensive transgenesis process is plagued by extensive timescales. Plant viruses potential to target specific genes and integrate exogenous DNA molecules at locus. Their ability manipulate genetic material become part it makes them remarkable agents helpful for molecular synthetic biology. In this review, we describe how geminivirus-based be utilized overcome transgenesis. We highlight progress has been made so far also discuss hurdles hinder employment vectors. Furthermore, conclude with comparison other plant-derived Geminivirus-based stand poised revolutionize plant genome editing making nucleic acid manipulation cheaper easier deploy, thus lessening major technical constraints, including homology-directed repair (HDR)-mediated time-inefficient tissue culture procedures. insights given review illustrate broader picture geminiviral vectors, emphasis on engineering ease practices crop improvements as well boost experimental timescales from years months.

Language: Английский