Epigenetic regulation and beyond in grapevine‐pathogen interactions: a biotechnological perspective DOI Creative Commons
João Proença Pereira, Ivan Bevilacqua, Rita B. Santos

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract As one of the most important crop plants worldwide, understanding mechanisms underlying grapevine response to pathogen attacks is key achieving a productive and sustainable viticulture. Recently, epigenetic regulation in plant immunity has gained significant traction scientific community, not only for its role gene expression but also heritability, giving it enormous biotechnological potential. Epigenetic marks have been shown be dynamically modulated genomic regions upon infection, with some being maintained after such, responsible priming defense genes. In grapevine, however, knowledge still limited, especially regarding biotic stress responses, representing glaring gap this plant. Here, we report integrate current on as well non‐epigenetic non‐coding RNAs stress. We explore how may useful breeding resistance, considering different approaches, from uncovering exploiting natural variation inducing through means.

Language: Английский

Advances in the molecular mechanism of grapevine resistance to fungal diseases DOI Creative Commons
Zhi Li, Ronghui Wu, Feng Guo

et al.

Molecular Horticulture, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 2, 2025

Abstract Grapevine is an important economic fruit tree worldwide, but grape production has been plagued by a vast number of fungal diseases, which affect vigor and the quality yield berries. To seek remedies for such issues, researchers have always committed to conventional biotechnological breeding. In recent years, increasing progress made in elucidating molecular mechanisms grape–pathogenic fungi interactions resistance regulation. Here, we summarize current knowledge on basis grapevine including effector-mediated susceptibility resistance, resistant regulatory networks grapevine, innovative approaches genetic transformation, strategies improve resistance. Understanding exploring accurately regulating diseases.

Language: Английский

Citations

2
Simplified Protocol for the Purification of Native Cas Nucleases for DNA-Free Genome Editing DOI Creative Commons
Margherita D’Amico, Flavia Angela Maria Maggiolini, Lucia Rosaria Forleo

et al.

Methods and Protocols, Journal Year: 2025, Volume and Issue: 8(1), P. 16 - 16

Published: Feb. 7, 2025

DNA-free genome editing by the direct delivery of CRISPR-associated nucleases has emerged as a promising technology due to its precision and reduced risk off-target effects. However, existing purification protocols for native Cas proteins require use complex instrumentation, which limits their application. Here, we present simplified protocol Cas9, Cas12RR dCas9-VP64 optimized editing. Our approach leverages streamlined affinity ion exchange chromatography coupled with minimal downstream processing, ensuring good yield activity purified proteins. The in vitro analysis ribonucleoprotein demonstrated efficiency DNA target cleavage. This increases opportunity adopt CRISPR technology, enables broader access tools also laboratories that are not specifically equipped protein purification.

Language: Английский

Citations

0
Epigenetic regulation and beyond in grapevine‐pathogen interactions: a biotechnological perspective DOI Creative Commons
João Proença Pereira, Ivan Bevilacqua, Rita B. Santos

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract As one of the most important crop plants worldwide, understanding mechanisms underlying grapevine response to pathogen attacks is key achieving a productive and sustainable viticulture. Recently, epigenetic regulation in plant immunity has gained significant traction scientific community, not only for its role gene expression but also heritability, giving it enormous biotechnological potential. Epigenetic marks have been shown be dynamically modulated genomic regions upon infection, with some being maintained after such, responsible priming defense genes. In grapevine, however, knowledge still limited, especially regarding biotic stress responses, representing glaring gap this plant. Here, we report integrate current on as well non‐epigenetic non‐coding RNAs stress. We explore how may useful breeding resistance, considering different approaches, from uncovering exploiting natural variation inducing through means.

Language: Английский

Citations

0