Differential mechanisms of cold sensitivity in mouse trigeminal and vagal ganglion neurons DOI Creative Commons
Katharina Gers-Barlag, Pablo Hernández‐Ortego,

Eva Quintero

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Aug. 6, 2021

Abstract Thermal signals are critical elements in the operation of interoceptive and exteroceptive neural circuits, essential for triggering thermally-driven reflexes conscious behaviors. A fraction cutaneous visceral sensory endings activated by cold temperatures. Compared to somatic (DRG TG) neurons, little is known about mechanisms underlying sensitivity vagal neurons. We used molecular, pharmacological genetic tools a side-by-side characterization cold-sensitive (CS) neurons adult mouse trigeminal (TG) ganglia (VG). found that CS larger size more abundant VG than TG. In VG, majority co-express TRPA1 markers cold-evoked responses severely blunted Trpa1 KO mice. Cold was evident with highest expression. contrast, TRPM8 deletion or blockade had impact on sensitivity. Consistent these findings, Trpm8 eYFP reporter mice we limited expression restricted rostral jugular ganglion. vivo retrograde labelling airway-innervating demonstrated their enhanced higher compared innervating stomach wall. TG reduced after blockade. However, reduction showed channels contribute significantly high-threshold TG, suggestive role noxious sensing. both ganglia, responded cooling mechanism independent yet be characterized. Finally, varied widely potassium channel blocker 4-AP. This effect sensor expressed neuron, common excitability brake mechanism. Significance statement Temperature sensing its regulation homeostatic function. Little molecular relative weight different organs. study highlights important differences thermotransduction between (trigeminal) (vagal) primary establishing transduction. The describes quantitative lower airways, suggesting transduction may fine-tuned specific needs advances our understanding reveals distinct drug targets modulation thermoreceptors.

Language: Английский

Interoception in Parkinson's disease: A narrative review and framework for translational research DOI
Katherine Longardner, Senegal Alfred Mabry, Gloria Chen

et al.

Autonomic Neuroscience, Journal Year: 2025, Volume and Issue: 259, P. 103258 - 103258

Published: March 8, 2025

Language: Английский

Citations

0
Mechanosensitive release of ATP in the urinary bladder mucosa DOI Creative Commons
Violeta N. Mutafova‐Yambolieva

Purinergic Signalling, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

The urinary bladder mucosa (urothelium and suburothelium/lamina propria) functions as a barrier between the content of urine underlying tissue. is also mechanosensitive tissue that releases signaling molecules affect cells in wall interconnecting with detrusor muscle CNS. Adenosine 5ʹ-triphosphate (ATP) primary mechanotransduction signal released from response to distention activates cell membrane-localized P2X P2Y purine receptors on urothelial cells, sensory efferent neurons, interstitial smooth cells. amounts ATP at active receptor sites depend significantly extracellularly ATP. Spontaneous distention-induced release appear be under differential control. This review focused mechanisms mechanical stimulation. First, we present brief overview studies report or tissues. Then, discuss experimental evidence for by vesicular non-vesicular roles stretch-activated channels PIEZO channels, transient potential vanilloid type 4, pannexin 1. followed discussion possible involvement calcium homeostasis modulator 1, acid-sensing connexins We conclude limitations current research needs further increase our understanding purinergic regulation function.

Language: Английский

Citations

1
Topical reviews, with tropical views DOI
Vaughan G. Macefield

The Journal of Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

Language: Английский

Citations

0
Differential mechanisms of cold sensitivity in mouse trigeminal and vagal ganglion neurons DOI Creative Commons
Katharina Gers-Barlag, Pablo Hernández‐Ortego,

Eva Quintero

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Aug. 6, 2021

Abstract Thermal signals are critical elements in the operation of interoceptive and exteroceptive neural circuits, essential for triggering thermally-driven reflexes conscious behaviors. A fraction cutaneous visceral sensory endings activated by cold temperatures. Compared to somatic (DRG TG) neurons, little is known about mechanisms underlying sensitivity vagal neurons. We used molecular, pharmacological genetic tools a side-by-side characterization cold-sensitive (CS) neurons adult mouse trigeminal (TG) ganglia (VG). found that CS larger size more abundant VG than TG. In VG, majority co-express TRPA1 markers cold-evoked responses severely blunted Trpa1 KO mice. Cold was evident with highest expression. contrast, TRPM8 deletion or blockade had impact on sensitivity. Consistent these findings, Trpm8 eYFP reporter mice we limited expression restricted rostral jugular ganglion. vivo retrograde labelling airway-innervating demonstrated their enhanced higher compared innervating stomach wall. TG reduced after blockade. However, reduction showed channels contribute significantly high-threshold TG, suggestive role noxious sensing. both ganglia, responded cooling mechanism independent yet be characterized. Finally, varied widely potassium channel blocker 4-AP. This effect sensor expressed neuron, common excitability brake mechanism. Significance statement Temperature sensing its regulation homeostatic function. Little molecular relative weight different organs. study highlights important differences thermotransduction between (trigeminal) (vagal) primary establishing transduction. The describes quantitative lower airways, suggesting transduction may fine-tuned specific needs advances our understanding reveals distinct drug targets modulation thermoreceptors.

Language: Английский

Citations

0