Spatio-temporal dynamics of the fibrotic niche in cardiac repair DOI Creative Commons
Andy Chan, Joachim Greiner,

Tomás A. Brennan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Summary The heart is one of the least regenerative organs in humans, and ischemic disease leading cause death worldwide. Understanding cellular molecular processes that occur during cardiac wound healing an essential prerequisite to reducing health burden improve function after myocardial tissue damage. By integrating single-cell RNA-sequencing with imaging-based spatial transcriptomics, we reconstructed spatio-temporal dynamics fibrotic niche ventricular injury adult mice. Our analysis reveals dynamic regulation local cell communication niches over time. We identified interactions regulate repair, including fibroblast proliferation silencing by Trem2 high macrophages prevents excessive fibrosis. Moreover, discovered a rare population dedifferentiating cardiomyocytes early post-lesion stages, which was sustained signals from myeloid lymphoid cells. Culturing non-regenerative mouse or human these factors reactivated progenitor gene expression cycle activity. In summary, this type atlas provides valuable insights into heterocellular control repair. Highlights scRNA-seq situ sequencing reveal repair Local coordinate overall response Fibroblast suppresses fibrosis Cardiomyocyte plasticity promoted cells

Language: Английский

Cardiac Macrophage: Insights from Murine Models to Translational Potential for Human Studies DOI
Yufeng Liu,

Tricia Wang,

Yongbo Lu

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cardiac macrophage: Insights from murine models to translational potential for human studies DOI
Yufeng Liu, Tricia T. Wang, Yongbo Lu

et al.

Journal of Molecular and Cellular Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Osteogenic differentiation of bone mesenchymal stem cells on linearly aligned triangular micropatterns DOI
Ye Zeng, Junyi Shen, Xintong Zhou

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(34), P. 8420 - 8430

Published: Jan. 1, 2024

The engineered surfaces, linearly aligned triangular micropatterns (TPs) with small interspaces, promote osteogenic differentiation in BMSCs via Piezo1-mediated myosin remodeling and YAP1 nuclear translocation. Cells were enlarged elongated.

Language: Английский

Citations

3
TRPV4—A Multifunctional Cellular Sensor Protein with Therapeutic Potential DOI Creative Commons

Sanna Koskimäki,

Sari Tojkander

Sensors, Journal Year: 2024, Volume and Issue: 24(21), P. 6923 - 6923

Published: Oct. 29, 2024

Transient receptor potential vanilloid (TRPV) channel proteins belong to the superfamily of TRP that form cationic channels in animal cell membranes. These have various subtype-specific functions, serving, for example, as sensors pain, pressure, pH, and mechanical extracellular stimuli. The sensing cues by TRPV4 triggers Ca2+-influx through channel, subsequently coordinating numerous intracellular signaling cascades a spatio-temporal manner. As TRPV play such wide role cellular physiological loss or impaired protein activity naturally contributes many pathophysiological processes. This review concentrates on known functions sensor their therapeutic target.

Language: Английский

Citations

2
Functional characterization of PIEZO1 in distinct macrophage populations DOI
Lia Farahi, Shirin Pourteymour

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 602(18), P. 4457 - 4459

Published: July 19, 2024

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by authors. Any queries (other than missing content) should be directed to corresponding author article.

Language: Английский

Citations

1
Cardiac mechano‐electric crosstalk: multi‐scale observations, computational integration, and clinical implications DOI
Ramona Emig, Eilidh A. MacDonald, T. Alexander Quinn

et al.

The Journal of Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Citations

1
Spatio-temporal dynamics of the fibrotic niche in cardiac repair DOI Creative Commons
Andy Chan, Joachim Greiner,

Tomás A. Brennan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Summary The heart is one of the least regenerative organs in humans, and ischemic disease leading cause death worldwide. Understanding cellular molecular processes that occur during cardiac wound healing an essential prerequisite to reducing health burden improve function after myocardial tissue damage. By integrating single-cell RNA-sequencing with imaging-based spatial transcriptomics, we reconstructed spatio-temporal dynamics fibrotic niche ventricular injury adult mice. Our analysis reveals dynamic regulation local cell communication niches over time. We identified interactions regulate repair, including fibroblast proliferation silencing by Trem2 high macrophages prevents excessive fibrosis. Moreover, discovered a rare population dedifferentiating cardiomyocytes early post-lesion stages, which was sustained signals from myeloid lymphoid cells. Culturing non-regenerative mouse or human these factors reactivated progenitor gene expression cycle activity. In summary, this type atlas provides valuable insights into heterocellular control repair. Highlights scRNA-seq situ sequencing reveal repair Local coordinate overall response Fibroblast suppresses fibrosis Cardiomyocyte plasticity promoted cells

Language: Английский

Citations

1