Sigh generation in preBötzinger Complex

Published: Jan. 3, 2025

We explored neural mechanisms underlying sighing. Photostimulation of parafacial (pF) neuromedin B ( NMB) or gastrin releasing peptide (GRP), preBötzinger Complex (preBötC) NMBR GRPR neurons elicited ectopic sighs with latency inversely related to time from preceding endogenous sigh. Of particular note, could be produced without involvement these peptides their receptors in preBötC. Moreover, chemogenetic optogenetic activation preBötC SST induced sighing, even the presence and/or antagonists. propose that an increase excitability not requiring activates partially overlapping pathways generate sighs, and are a downstream element sigh generation circuit converts normal breaths into sighs.

Language: Английский

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A Molecularly Defined Medullary Network for Control of Respiratory Homeostasis

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

The dynamic interaction between central respiratory chemoreceptors and the pattern generator constitutes a critical homeostatic axis for stabilizing breathing rhythm pattern, yet its circuit-level organization remains poorly characterized. Here, functional connectivity two key medullary hubs: nucleus tractus solitarius (NTS) preBötzinger complex (preBötC) are systematically investigated. These findings delineate network primarily comprising Phox2b-expressing NTS neurons (NTSPhox2b), GABAergic (NTSGABA), somatostatin (SST)-expressing preBötC (preBötCSST). Photostimulation of NTSPhox2b projecting to potently amplifies baseline ventilation, whereas genetic ablation these or knockout their transient receptor potential channel 5 (TRPC5) significantly blunts CO2-stimulated ventilatory responses. Conversely, NTSGABA neuron stimulation inhibits halts partially via monosynaptic inhibition preBötC. Additionally, photostimulation preBötCSST drives deep slow through coordinated modulation neurons. collectively identify an important that integrates chemosensory feedback with motor output, enabling tuning patterns metabolic demands.

Language: Английский

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Lethal Interactions of neuronal networks in epilepsy mediated by both synaptic and volume transmission indicate approaches to prevention

Progress in Neurobiology, Journal Year: 2025, Volume and Issue: 249, P. 102770 - 102770

Published: April 19, 2025

Language: Английский

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Sigh generation in preBötzinger Complex

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 9, 2024

We explored neural mechanisms underlying sighing. Photostimulation of parafacial (pF) neuromedin B (NMB) or gastrin releasing peptide (GRP), preBötzinger Complex (preBötC) NMBR GRPR neurons elicited ectopic sighs with latency inversely related to time from preceding endogenous sigh. Of particular note, could be produced without involvement these peptides their receptors in preBötC. Moreover, chemogenetic optogenetic activation preBötC SST induced sighing, even the presence and/or antagonists. propose that an increase excitability not requiring activates partially overlapping pathways generate sighs, and are a downstream element sigh generation circuit converts normal breaths into sighs.

Language: Английский

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Unique spatiotemporal synchronization solutions of heterogeneous local Ca²⁺dynamics underlie the formation of each impulse that emerges from the cardiac sinoatrial node

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Abstract Spontaneous, stochastic, local Ca 2+ oscillations (LCOs) that are heterogeneous in phase, frequency, and amplitude incessantly occur throughout the neuronal-like cytoarchitecture of heart’s pacemaker, sinoatrial node (SAN). How information encoded within ensembles these LCOs is linked to incessant emergence formation rhythmic SAN impulses, however, an unsolved problem. We applied a wide range analytical techniques adapted from dynamical systems neuroscience map fine details spatiotemporal distributions dynamics time-series high-speed acquisition panoramic images mouse SANs ex vivo. Phase analysis delineated network functional pacemaker cell clusters, which dynamic amplitudes, kinetics, phases were similar, but still differed each other, those other clusters. Local clusters formed global with mean rate identical recorded by reference sharp electrode right atria. Initial conditions impulse initiation due mostly stochastic nature small cluster located near crista terminalis, having highest intrinsic power, earliest rotor-like energy transfer, most frequent point-to-point instability, acrophase, greatest impulse-to-impulse variability A spontaneous, slowly increasing ensemble signal this forms foot CaT. Following foot, CaT increased rapidly, rapid increases kinetics amplitudes some adjacent In inter-atrial septum, delayed, increase occurred when relaxation phase had already begun. Orbit plots Lyapunov exponent analyses detected both deterministic features Ca2+ dynamics. Correlation cross-talk among different varied, not only impulse, also one next, forming unique small-world networks, assessed as ratios correlation coefficientsand shortest path lengths, ultimately creating synchronization solution for Thus, individual during cycle underlies length SAN, recapitulating spontaneous CaTs isolated, single, cells.

Language: Английский

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How neural networks walk and chew gum

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 602(5), P. 767 - 768

Published: Feb. 10, 2024

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by authors. Any queries (other than missing content) should be directed to corresponding author article.

Language: Английский

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The hypoxic respiratory response of the pre-Bötzinger complex

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34491 - e34491

Published: July 1, 2024

Language: Английский

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