Measurement and analysis of rare circulating tumor cell dynamics with diffuse in vivo flow cytometry DOI Open Access

Amber Luna Williams

Published: Jan. 1, 2024

Circulating tumor cells (CTCs) are shed from primary tumors to facilitate hematogenous metastasis. The method of studying CTCs is by analysis small, drawn blood samples, known as 'liquid biopsy'. However, little about fluctuations in the number these over time. Our lab recently developed diffuse vivo flow cytometry (DiFC) which continuously and non-invasively scans large vessels small animals detect rare circulating fluorescent cells. aim this dissertation was develop new DiFC technology methods apply study variability CTC numbers different time scales.First, we analyzed data sets measured multiple myeloma Lewis lung carcinoma mouse metastasis models vivo. continuous nature allows us equate detections short intervals captured samples. showed that samples unlikely accurately estimate burden across entire volume. Additionally, short-term variation much higher than previously assumed, suggesting shedding rates change course minutes or hours. Second, designed, built, validated a system can with two distinct fluorophores, for example green protein (GFP) tdTomato protein. This monitor populations concurrently same animal two-fluorophore clusters containing both populations. Finally, applied two-color multi-fluorophore breast cancer bearing mice. These increased grew, but growth not monotonic Both exhibited similar variation, demonstrating types have unknown behavior. Whole-body hyperspectral fluorescence cryo-imaging also used identify volume, location, distribution metastases. methodology has significant implications understanding dissemination further interpretation liquid biopsy pre-clinical research humans.--Author's abstract

Language: Английский

Rapid diffused optical imaging for accurate 3D estimation of subcutaneous tissue features DOI Creative Commons
Shanshan Cai, D. John,

Winn Hong

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(2), P. 111818 - 111818

Published: Jan. 23, 2025

Conventional light imaging in living tissues is limited to depths under 100 μm by the significant tissue scattering. Consequently, few commercial devices can image lesions beneath surface, or measure their invasion depth, critical dermatology. We present 3D-multisite diffused optical (3D-mDOI) an approach that combines photon migration techniques from diffuse tomography, with automated controls and analysis for estimating lesion's depth via its coefficients. 3D-mDOI a non-invasive, low-cost, fast, contact-free instrument capable of subcutaneous structures volumes through multisite-acquisition re-emitted diffusion on sample surface. It offers rapid estimation Breslow essential staging melanoma. To standardize performance, employs customized calibrations using physical phantoms, explore system's 3D reconstruction capabilities. find reconstruct up 5 mm below requiring ∼300 s computation time.

Language: Английский

Citations

0
Spatial sensitivity to absorption changes for various near-infrared spectroscopy methods: A compendium review DOI Creative Commons
Giles Blaney, Angelo Sassaroli, Sergio Fantini

et al.

Journal of Innovative Optical Health Sciences, Journal Year: 2024, Volume and Issue: 17(04)

Published: Feb. 24, 2024

This compendium review focuses on the spatial distribution of sensitivity to localized absorption changes in optically diffuse media, particularly for measurements relevant near-infrared spectroscopy. The three temporal domains, continuous-wave, frequency-domain, and time-domain, each obtain different optical data-types whose may be related effective homogeneous coefficient. Sensitivity is relationship between a perturbation recovered change. Therefore, maps representing location can generated numerous domains. first presents history past 30 years work investigating this media. These works are experimental theoretical, presenting 1-, 2-, 3-dimensional spectroscopy methods, data-types. Following history, we present organized by domain then data-type. provides valuable tool compare various measurement methods parameters one document. Methods generate these provided appendix, including code. historical comprehensive map single source researchers use visualize, investigate, compare, change maps.

Language: Английский

Citations

2
Two-color diffuse in vivo flow cytometer DOI Creative Commons
Amber Williams,

Augustino V. Scorzo,

Rendall R. Strawbridge

et al.

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(06)

Published: May 30, 2024

Hematogenous metastasis is mediated by circulating tumor cells (CTCs) and CTC clusters (CTCCs). We recently developed "diffuse

Language: Английский

Citations

2
Dual-ratio approach to pulse oximetry and the effect of skin tone DOI Creative Commons
Giles Blaney, Jodee Frias, Fatemeh Tavakoli

et al.

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S3)

Published: Oct. 12, 2024

SignificancePulsatile blood oxygen saturation (SpO2) via pulse oximetry is a valuable clinical metric for assessing delivery. Individual anatomical features, including skin tone, may affect current optical methods.AimWe developed an method based on dual-ratio (DR) measurements to suppress individual confounds SpO2.ApproachWe designed DR-based finger oximeter, hypothesizing that DR would from coupling and superficial tissue absorption. This tested using Monte Carlo simulations in vivo experiments.ResultsDifferent melanosome volume fractions the epidermis, surrogate cause changes recovered SpO2 order of 1% simulation vivo. Different heterogeneous pulsatile hemodynamics greater 10% simulations. with showed less variability than traditional single-distance (SD) transmission method.ConclusionsFor models methods considered here, are strongly impacted by hemodynamics. be larger tone bias, which known confound measurements. The partial suppression suggests promise oximetry.

Language: Английский

Citations

2
Measurement and analysis of rare circulating tumor cell dynamics with diffuse in vivo flow cytometry DOI Open Access

Amber Luna Williams

Published: Jan. 1, 2024

Circulating tumor cells (CTCs) are shed from primary tumors to facilitate hematogenous metastasis. The method of studying CTCs is by analysis small, drawn blood samples, known as 'liquid biopsy'. However, little about fluctuations in the number these over time. Our lab recently developed diffuse vivo flow cytometry (DiFC) which continuously and non-invasively scans large vessels small animals detect rare circulating fluorescent cells. aim this dissertation was develop new DiFC technology methods apply study variability CTC numbers different time scales.First, we analyzed data sets measured multiple myeloma Lewis lung carcinoma mouse metastasis models vivo. continuous nature allows us equate detections short intervals captured samples. showed that samples unlikely accurately estimate burden across entire volume. Additionally, short-term variation much higher than previously assumed, suggesting shedding rates change course minutes or hours. Second, designed, built, validated a system can with two distinct fluorophores, for example green protein (GFP) tdTomato protein. This monitor populations concurrently same animal two-fluorophore clusters containing both populations. Finally, applied two-color multi-fluorophore breast cancer bearing mice. These increased grew, but growth not monotonic Both exhibited similar variation, demonstrating types have unknown behavior. Whole-body hyperspectral fluorescence cryo-imaging also used identify volume, location, distribution metastases. methodology has significant implications understanding dissemination further interpretation liquid biopsy pre-clinical research humans.--Author's abstract

Language: Английский

Citations

0