Synaptic and Somatic Targeting of ArcLight, a Genetically Encoded Voltage Indicator DOI Open Access
Sheng Zhong, Lawrence B. Cohen

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

ABSTRACT Voltage signals in neurons are highly compartmentalized, which can influence their specific functions within neuronal circuits. Targeting of a genetically encoded voltage indicator (GEVI) to subcellular compartments enhance the signal-to-noise ratio and provide more precise information about location timing synaptic firing across different regions, reducing spatiotemporal signal convolution. To achieve targeting GEVI, ArcLight, we utilized five postsynaptic sequences ( Shaker K + channel C-terminus, stargazin rat Neuroligin-1 anti-homer1 nanobodies HC20 & HC87) direct ArcLight expression excitatory density. Additionally, assessed presynaptic-targeting tag (rat Neurexin-1β C-terminus) somatodendritic (Kv2.1-Lk-Tlcn C-terminus). Patch clamp experiments HEK293 cells showed that tags used this study did not significantly alter ArcLight’s sensitivity compared controls. AAV infection mouse olfactory bulb demonstrated effectively localized GEVI mitral/tufted cells, including densities, presynaptic terminals, regions. Furthermore, i n vivo imaging mice expressing targeting-enhanced variants revealed odorant-evoked responses similar those observed with original ArcLight. This indicates impact sensing capability cells.

Language: Английский

Odor representation and coding by the mitral/tufted cells in the olfactory bulb DOI
Pan Ke Wang, Shan Li, Anan Li

et al.

Journal of Zhejiang University SCIENCE B, Journal Year: 2024, Volume and Issue: 25(10), P. 824 - 840

Published: Oct. 1, 2024

Language: Английский

Citations

1
Recent odor experience selectively modulates olfactory sensitivity across the glomerular output in the mouse olfactory bulb DOI Creative Commons
Narayan Subramanian, Lee Min Leong,

Paria Salemi Mokri Boukani

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Abstract Although animals can reliably locate and recognize odorants embedded in complex environments, the neural circuits for accomplishing these tasks remain incompletely understood. Adaptation is likely to be important as it could allow neurons a brain area adjust broader sensory environment. Adaptive processes must flexible enough make dynamic adjustments, while maintaining sufficient stability so that organisms do not forget olfactory associations. Processing within mouse bulb involved generating adaptation, although there are conflicting models of how transforms glomerular output bulb. Here we performed 2-photon Ca 2+ imaging from mitral/tufted glomeruli awake mice determine time course recovery whether acts broadly or selectively across population. Individual responses, well overall population odor representation was similar sessions. However, odor-concentration pairings presented with interstimulus intervals upwards 30-s evoked heterogeneous adaptation concentration-dependent. We demonstrate this form unrelated variations respiration, receptor neuron measurements indicate unlikely inherited periphery. Our results transmit stable representations, but recent experiences shape responsiveness 30 seconds. propose non-uniform making adjustments environments.

Language: Английский

Citations

0
Synaptic and Somatic Targeting of ArcLight, a Genetically Encoded Voltage Indicator DOI Open Access
Sheng Zhong, Lawrence B. Cohen

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

ABSTRACT Voltage signals in neurons are highly compartmentalized, which can influence their specific functions within neuronal circuits. Targeting of a genetically encoded voltage indicator (GEVI) to subcellular compartments enhance the signal-to-noise ratio and provide more precise information about location timing synaptic firing across different regions, reducing spatiotemporal signal convolution. To achieve targeting GEVI, ArcLight, we utilized five postsynaptic sequences ( Shaker K + channel C-terminus, stargazin rat Neuroligin-1 anti-homer1 nanobodies HC20 & HC87) direct ArcLight expression excitatory density. Additionally, assessed presynaptic-targeting tag (rat Neurexin-1β C-terminus) somatodendritic (Kv2.1-Lk-Tlcn C-terminus). Patch clamp experiments HEK293 cells showed that tags used this study did not significantly alter ArcLight’s sensitivity compared controls. AAV infection mouse olfactory bulb demonstrated effectively localized GEVI mitral/tufted cells, including densities, presynaptic terminals, regions. Furthermore, i n vivo imaging mice expressing targeting-enhanced variants revealed odorant-evoked responses similar those observed with original ArcLight. This indicates impact sensing capability cells.

Language: Английский

Citations

0