Demixing fluorescence time traces transmitted by multimode fibers

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 26, 2024

Abstract Optical methods based on thin multimode fibers (MMFs) are promising tools for measuring neuronal activity in deep brain regions of freely moving mice thanks to their small diameter. However, current limited: while fiber photometry provides only ensemble activity, imaging techniques using long very sensitive bending and have not been applied unrestrained rodents yet. Here, we demonstrate the fundamentals a new approach short MMF coupled miniscope. In proof-of-principle vitro experiments, disentangled spatio-temporal fluorescence signals from multiple fluorescent sources transmitted by (200 µm) (8 mm) MMF, general unconstrained non-negative matrix factorization algorithm directly raw video data. Furthermore, show that low-cost open-source miniscopes sufficient sensitivity image same patterns seen our experiment, suggesting avenue novel minimally invasive studies behaving mice.

Language: Английский

Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский

Focusing through Scattering Media Using Integrated Photonics

ACS Photonics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Language: Английский