NaHS@Cy5@MS@SP nanoparticles improve rheumatoid arthritis by inactivating the Hedgehog signaling pathway through sustained and targeted release of H2S into the synovium DOI Creative Commons
Xuexue Zhu,

An‐Jing Xu,

Weiwei Cai

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 8, 2025

Aberrant proliferation and inflammation of fibroblast-like synoviocytes (FLSs) significantly contribute to the pathogenesis rheumatoid arthritis (RA). Deficiency hydrogen sulfide (H2S) is a driving force for development RA, short half-life H2S-releasing donor sodium hydrosulfide (NaHS) limits its clinical application in RA therapy. Designing targeted delivery system with slow-release properties FLSs could offer novel strategies treating RA. Herein, we designed strategy achieve slow release H2S synovium, which was accomplished by synthesizing NaHS-CY5@mesoporous silic@LNP peptide Dil (NaHS@Cy5@MS@SP) nanoparticles. Our results demonstrated that NaHS@Cy5@MS@SP effectively targets FLSs, upregulates its-producing enzyme cystathionine-γ-lyase (CSE) joints arthritic mice. Overexpression CSE inhibited proliferation, migration, upon lipopolysaccharide (LPS) exposure, effects were mimicked NaHS@Cy5@MS@SP. In vivo studies showed achieved threefold higher AUCinf than free NaHS, improving bioavailability NaHS. Further, synovial hyperplasia reduced bone cartilage erosion DBA/1J mouse model collagen-induced (CIA), superior RNA sequencing molecular validated inactivated Hedgehog signaling pathway as evidenced reductions protein expression SHH, SMO, GLI1 phosphorylated p38/MAPK. This study highlights promising controlled synoviocytes, offering potential management.

Language: Английский

A Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detecting Hydrogen Sulfide in Environmental Waters, Wine Samples, and Living Systems DOI
Shuai Gong, Yue Gu,

Jixiang Tian

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Hydrogen sulfide (H2S) is closely related to physiological diseases, environmental pollution, food safety, and plant growth. Herein, a new nopinone-based near-infrared fluorescent probe has been developed detect H2S with superior performance, including large Stokes shift, low detection limit, good selectivity, fast response time. NPD-H2S was used in water wine samples quantitatively. In addition, could be fabricate nanofiber membranes as portable tool for sensitively detecting vapor using smartphone. Moreover, effectively image HeLa cells, MCF-7 zebrafish. More importantly, the monitored presence of upregulation processes Arabidopsis thaliana under salt stress by bioimaging. This work provides concept intervention external stresses cultivation. Therefore, can monitor fluctuation environment living systems.

Language: Английский

Citations

0
NaHS@Cy5@MS@SP nanoparticles improve rheumatoid arthritis by inactivating the Hedgehog signaling pathway through sustained and targeted release of H2S into the synovium DOI Creative Commons
Xuexue Zhu,

An‐Jing Xu,

Weiwei Cai

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 8, 2025

Aberrant proliferation and inflammation of fibroblast-like synoviocytes (FLSs) significantly contribute to the pathogenesis rheumatoid arthritis (RA). Deficiency hydrogen sulfide (H2S) is a driving force for development RA, short half-life H2S-releasing donor sodium hydrosulfide (NaHS) limits its clinical application in RA therapy. Designing targeted delivery system with slow-release properties FLSs could offer novel strategies treating RA. Herein, we designed strategy achieve slow release H2S synovium, which was accomplished by synthesizing NaHS-CY5@mesoporous silic@LNP peptide Dil (NaHS@Cy5@MS@SP) nanoparticles. Our results demonstrated that NaHS@Cy5@MS@SP effectively targets FLSs, upregulates its-producing enzyme cystathionine-γ-lyase (CSE) joints arthritic mice. Overexpression CSE inhibited proliferation, migration, upon lipopolysaccharide (LPS) exposure, effects were mimicked NaHS@Cy5@MS@SP. In vivo studies showed achieved threefold higher AUCinf than free NaHS, improving bioavailability NaHS. Further, synovial hyperplasia reduced bone cartilage erosion DBA/1J mouse model collagen-induced (CIA), superior RNA sequencing molecular validated inactivated Hedgehog signaling pathway as evidenced reductions protein expression SHH, SMO, GLI1 phosphorylated p38/MAPK. This study highlights promising controlled synoviocytes, offering potential management.

Language: Английский

Citations

0