The Microbial Connection to Sustainable Agriculture DOI Creative Commons
Kalaivani Nadarajah, Nur Sabrina Natasha Abdul Rahman

Plants, Journal Year: 2023, Volume and Issue: 12(12), P. 2307 - 2307

Published: June 14, 2023

Microorganisms are an important element in modeling sustainable agriculture. Their role soil fertility and health is crucial maintaining plants' growth, development, yield. Further, microorganisms impact agriculture negatively through disease emerging diseases. Deciphering the extensive functionality structural diversity within plant-soil microbiome necessary to effectively deploy these organisms Although both plant have been studied over decades, efficiency of translating laboratory greenhouse findings field largely dependent on ability inoculants or beneficial colonize maintain stability ecosystem. its environment two variables that influence microbiome's structure. Thus, recent years, researchers looked into engineering would enable them modify microbial communities order increase effectiveness inoculants. The environments believed support resistance biotic abiotic stressors, fitness, productivity. Population characterization manipulation, as well identification potential biofertilizers biocontrol agents. Next-generation sequencing approaches identify culturable non-culturable microbes associated with expanded our knowledge this area. Additionally, genome editing multidisciplinary omics methods provided scientists a framework engineer dependable high yield, resistance, nutrient cycling, management stressors. In review, we present overview agriculture, engineering, translation technology field, main used by laboratories worldwide study microbiome. These initiatives advancement green technologies

Language: Английский

The rhizosphere microbiome plays a role in the resistance to soil-borne pathogens and nutrient uptake of strawberry cultivars under field conditions DOI Creative Commons
Cristina Lazcano, Eric S. Boyd, Gerald J. Holmes

et al.

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Feb. 4, 2021

Microbial-root associations are important to help plants cope with abiotic and biotic stressors. Managing these interactions offers an opportunity for improving the efficiency sustainability of agricultural production. By characterizing bacterial archaeal community (via 16S rRNA sequencing) associated bulk rhizosphere soil sixteen strawberry cultivars in two controlled field studies, we explored relationships between microbiome plant resistance soil-borne fungal pathogens (Verticillium dahliae Macrophomina phaseolina). Overall, had a distinctive genotype-dependent higher abundances known beneficial bacteria such as Pseudomonads Rhizobium. The played significant role shown by differences high low cultivars. Resistant were characterized biocontrol microorganisms including actinobacteria (Arthrobacter, Nocardioides Gaiella) unclassified acidobacteria (Gp6, Gp16 Gp4), both pathogen trials. Additionally, that resistant V. Burkholderia M. phaseolina Pseudomonas. mechanisms involved plant-microbial their plasticity different environments should be studied further design low-input disease management strategies.

Language: Английский

Citations

161
Persistence of plant-mediated microbial soil legacy effects in soil and inside roots DOI Creative Commons
S. Emilia Hannula, Robin Heinen, Martine Huberty

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Sept. 28, 2021

Abstract Plant-soil feedbacks are shaped by microbial legacies that plants leave in the soil. We tested persistence of these after subsequent colonization same or other plant species using 6 typical grassland species. Soil fungal were detectable for months, but current effect on fungi amplified time. By contrast, bacterial communities, faded away rapidly and bacteria communities influenced strongly plant. However, both conserved inside roots their composition significantly correlated with growth. Hence, soil present at time establishment play a vital role shaping growth even when have due conclude microbiome reversible versatile, they can create plant-soil via altering endophytic community acquired during early ontogeny.

Language: Английский

Citations

152
Soil Acidification caused by excessive application of nitrogen fertilizer aggravates soil-borne diseases: Evidence from literature review and field trials DOI
Yijie Zhang, Chen Ye,

Yingwei Su

et al.

Agriculture Ecosystems & Environment, Journal Year: 2022, Volume and Issue: 340, P. 108176 - 108176

Published: Sept. 13, 2022

Language: Английский

Citations

152
The phyllosphere microbiome shifts toward combating melanose pathogen DOI Creative Commons

Pudong Li,

Zeng‐Rong Zhu, Yunzeng Zhang

et al.

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: April 2, 2022

Plants can recruit beneficial microbes to enhance their ability defend against pathogens. However, in contrast the intensively studied roles of rhizosphere microbiome suppressing plant pathogens, collective community-level change and effect phyllosphere response pathogen invasion remains largely elusive.Here, we integrated 16S metabarcoding, shotgun metagenomics culture-dependent methods systematically investigate changes between infected uninfected citrus leaves by Diaporthe citri, a fungal causing melanose disease worldwide. Multiple features suggested shift upon D. citri infection, highlighted marked reduction community evenness, emergence large numbers new microbes, intense microbial network. We also identified from functional perspectives leaves, such as enriched functions for iron competition potential antifungal traits, with genomic characteristics. Glasshouse experiments demonstrated that several bacteria associated could positively affect performance under challenge, reductions index ranging 65.7 88.4%. Among them, Pantoea asv90 Methylobacterium asv41 "recruited microbes" exhibited antagonistic activities both vitro vivo, including inhibition spore germination and/or mycelium growth. Sphingomonas spp. presented characteristics were found be main contributor enrichment complex outer membrane receptor protein leaves. Moreover, asv20 showed stronger suppression iron-deficient conditions than iron-sufficient conditions, suggesting role during action.Overall, our study revealed how microbiomes differed pathogen, mechanisms observed might have helped plants cope pressure. Our findings provide novel insights into understanding responses challenge. Video abstract.

Language: Английский

Citations

136
Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease DOI Creative Commons
Xin Zhou, Jinting Wang, Fang Liu

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Dec. 22, 2022

Abstract The role of rhizosphere microbiota in the resistance tomato plant against soil-borne Fusarium wilt disease (FWD) remains unclear. Here, we showed that FWD incidence was significantly negatively correlated with diversity both bacterial and fungal communities. Using microbiological culturomic approach, selected 205 unique strains to construct different synthetic communities (SynComs), which were inoculated into germ-free seedlings, their roles suppressing monitored using omics approach. Cross-kingdom (fungi bacteria) SynComs most effective than those Fungal or Bacterial alone. This effect underpinned by a combination molecular mechanisms related immunity microbial interactions contributed study provides new insight dynamics pathogen suppression host interactions. Also, formulation manipulation for functional complementation constitute beneficial strategy controlling disease.

Language: Английский

Citations

120
Rhizosphere Microbiome: The Emerging Barrier in Plant-Pathogen Interactions DOI Creative Commons
Jingtao Li, Chenyang Wang, Wenxing Liang

et al.

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 29, 2021

In the ecosystem, microbiome widely exists in soil, animals, and plants. With rapid development of computational biology, sequencing technology omics analysis, important role soil beneficial microbial community is being revealed. this review, we mainly summarized roles rhizosphere microbiome, revealing its complex pervasive nature contributing to largely invisible interaction with The manipulated microorganisms function as an indirect layer plant immune system by acting a barrier pathogen invasion or inducing systemic resistance. Specifically, could change recruit communities through root-type-specific metabolic properties, positively shape their response invasion. Meanwhile, plants microbes exhibit abilities avoid excessive responses for reciprocal symbiosis. Substantial lines evidence show pathogens might utilize secreting proteins/effectors overcome emerging peripheral advantage turn. Overall, are involved plant–pathogen interactions, power potential explored explained aim effectively increase growth productivity.

Language: Английский

Citations

115
Rhizospheric microbiome: Bio-based emerging strategies for sustainable agriculture development and future perspectives DOI Creative Commons
Kailash Chand Kumawat,

Nadia Razdan,

Krishna Saharan

et al.

Microbiological Research, Journal Year: 2021, Volume and Issue: 254, P. 126901 - 126901

Published: Oct. 25, 2021

Language: Английский

Citations

105
Exploring rhizo-microbiome transplants as a tool for protective plant-microbiome manipulation DOI Creative Commons
Gaofei Jiang, Yuling Zhang, Guiyun Gan

et al.

ISME Communications, Journal Year: 2022, Volume and Issue: 2(1)

Published: Feb. 1, 2022

The development of strategies for effectively manipulating and engineering beneficial plant-associated microbiomes is a major challenge in microbial ecology. In this sense, the efficacy potential implications rhizosphere microbiome transplant (RMT) plant disease management have only scarcely been explored literature. Here, we initially investigated differences 12 Solanaceae eggplant varieties accessed their level resistance promoted against bacterial wilt caused by pathogen Ralstonia solanacearum, 3-year field trial. We elected 6 resistant further tested broad feasibility using RMT from these donor to susceptible model tomato variety MicroTom. Overall, found enrich distinct specific taxa, which some displayed significant associations with suppression. Quantification source tracking analysis revealed more than 60% communities successfully colonize establish recipient plants. RTM donors resulted different levels suppression, reaching up 47% reduction incidence. Last, provide culture-dependent validation taxa associated antagonistic interactions pathogen, thus contributing better understanding mechanism Our study shows appropriate be promising tool modulate protective promote health. Together advocate future studies aiming at ecological processes mechanisms mediating rates coalescence between rhizosphere.

Language: Английский

Citations

93
Small changes in rhizosphere microbiome composition predict disease outcomes earlier than pathogen density variations DOI Creative Commons
Yian Gu, Samiran Banerjee, Francisco Dini‐Andreote

et al.

The ISME Journal, Journal Year: 2022, Volume and Issue: 16(10), P. 2448 - 2456

Published: July 22, 2022

Abstract Even in homogeneous conditions, plants facing a soilborne pathogen tend to show binary outcome with individuals either remaining fully healthy or developing severe lethal disease symptoms. As the rhizosphere microbiome is major determinant of plant health, we postulated that such may result from an early divergence assembly further cascade into varying suppression abilities. We tested this hypothesis by setting up longitudinal study tomato growing natural but homogenized soil infested bacterial Ralstonia solanacearum. Starting originally identical species pool, individual compositions rapidly diverged multiple configurations during vegetative growth. This variation community composition was strongly associated later development fruiting state. Most interestingly, these patterns also significantly predicted outcomes 2 weeks before any difference density became apparent between and diseased groups. In system, total 135 OTUs were persistent plants. Five enriched (Lysinibacillus, Pseudarthrobacter, Bordetella, Bacillus, Chryseobacterium) isolated shown reduce severity 30.4–100% when co-introduced pathogen. Overall, our results demonstrated initially can diverge microbiomes their ability promote protection. suggests life interventions have significant effects on states, highlights exciting opportunity for diagnostics prevention.

Language: Английский

Citations

93
Plant microbiota dysbiosis and the Anna Karenina Principle DOI Creative Commons
Gontran Arnault, Cendrine Mony, Philippe Vandenkoornhuyse

et al.

Trends in Plant Science, Journal Year: 2022, Volume and Issue: 28(1), P. 18 - 30

Published: Sept. 17, 2022

Language: Английский

Citations

85