ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 7, 2025
Biofilm-induced
chronic
bacterial
infections
represent
a
significant
challenge
in
modern
medicine
due
to
their
resistance
conventional
antibiotic
treatments.
Although
photodynamic
therapy
(PDT)
has
emerged
as
promising
antibiotic-free
antibacterial
strategy,
the
hypoxic
condition
within
biofilms
and
lack
of
an
effective
local
drug
delivery
system
have
limited
clinical
effectiveness
photosensitizer
(PS)
agents.
Herein,
we
propose
type
charge
regulation-enhanced
I
PS-loaded
hydrogel
dressing
for
treating
biofilm
infection.
The
regulation
enables
multiple
alkylation
Nile
blue
(EB
series)
exhibit
substantially
improved
absorbance
(∼2-fold),
alkaline
tolerance,
superoxide
anion
yield
(2.2-4.2-fold)
compared
representative
PS,
sulfur-substituted
blue.
Specifically,
enhanced
electronic
push-pull
capabilities
promote
more
efficient
electron
recycling
process,
significantly
boosting
efficiency
PDT.
superior
PDT
effect
uptake
via
render
EB
series
pronounced
inhibition
under
red
light
or
sunlight
irradiation.
Moreover,
hydrogel,
constructed
from
oxidized
dextran
quaternized
chitosan,
facilitates
localization
sustained
retention
PSs,
accelerating
healing
biofilm-infected
wounds.
This
PS-based
could
provide
user-friendly
wound
treatment
prevention
infections.
Small,
Journal Year:
2021,
Volume and Issue:
17(13)
Published: March 10, 2021
Abstract
Clinically,
inhibition
of
both
bacterial
infection
and
excessive
inflammation
is
a
crucial
step
for
improved
wound
treatments.
Herein,
the
fabrication
near‐infrared‐light
(NIR)‐activatable
deoxyribonuclease
(DNase)–carbon
monoxide
(CO)@mesoporous
polydopamine
nanoparticles
(MPDA
NPs)
demonstrated
efficient
elimination
methicillin‐resistant
Staphylococcus
aureus
(MRSA)
biofilms
following
anti‐inflammatory
activity.
Specifically,
thermosensitive
CO‐gas‐releasing
donors
(CO
releasing
molecules,
FeCO)
are
first
encapsulated
into
MPDA
NPs,
followed
by
covalently
immobilizing
I
(DNase
I)
on
surfaces
NPs.
DNase
can
degrade
extracellular
DNA
in
biofilms,
which
site
specifically
destroys
compactness
biofilms.
With
NIR
irradiation,
DNase–CO@MPDA
NPs
display
great
photothermal
ability,
further
trigger
on‐demand
delivery
bactericidal
CO
gas
that
adequately
permeate
impaired
Eventually,
they
achieve
effective
MRSA
biofilm
virtue
synergistic
effects
participation
CO‐gas‐potentiated
therapy.
Importantly,
inflammatory
responses
NIR‐treated
wounds
simultaneously
alleviated
owing
to
features
released
CO.
Finally,
NIR‐activatable
accelerate
healing
process
MRSA‐biofilm‐infected
cutaneous
wounds.
Taken
together,
this
phototherapeutic
strategy
displays
therapeutic
potential
treating
formidable
clinical
problems
caused
accompanying
inflammation.
Research,
Journal Year:
2022,
Volume and Issue:
2022
Published: Jan. 1, 2022
Three-dimensional
(3D)
bioprinting
has
been
extensively
explored
for
tissue
repair
and
regeneration,
while
the
insufficient
nutrient
oxygen
availability
in
printed
constructs,
as
well
lack
of
adaptive
dimensions
shapes,
compromises
overall
therapeutic
efficacy
limits
their
further
application.
Herein,
inspired
by
natural
symbiotic
relationship
between
salamanders
algae,
we
present
novel
living
photosynthetic
scaffolds
using
an
situ
microfluidic-assisted
3D
strategy
adapting
irregular-shaped
wounds
promoting
healing.
As
oxygenic
photosynthesis
unicellular
microalga
(Chlorella
pyrenoidosa)
was
incorporated
during
printing,
generated
could
produce
sustainable
under
light
illumination,
which
facilitated
cell
proliferation,
migration,
differentiation
even
hypoxic
conditions.
Thus,
when
microalgae-laden
were
directly
into
diabetic
wounds,
they
significantly
accelerate
chronic
wound
closure
alleviating
local
hypoxia,
increasing
angiogenesis,
extracellular
matrix
(ECM)
synthesis.
These
results
indicate
that
microalgae
offers
effective
autotrophic
biosystem
healing,
suggesting
a
promising
diverse
engineering
applications.
Bioactive Materials,
Journal Year:
2022,
Volume and Issue:
19, P. 653 - 665
Published: May 11, 2022
Wound
healing
is
one
of
the
major
global
health
concerns
in
patients
with
diabetes.
Overactivation
pro-inflammatory
M1
macrophages
associated
delayed
wound
miR-29ab1
plays
a
critical
role
diabetes-related
macrophage
inflammation.
Hence,
inhibition
inflammation
and
regulation
miR-29
expression
have
been
implicated
as
new
points
for
skin
healing.
In
this
study,
traditional
Chinese
medicine,
puerarin,
was
introduced
to
construct
an
injectable
self-healing
chitosan@puerarin
(C@P)
hydrogel.
The
C@P
hydrogel
promoted
diabetic
accelerated
angiogenesis,
which
were
related
mediated
response.
Compared
healthy
subjects,
miR-29a
miR-29b1
ectopically
increased
model,
accompanied
by
upregulated
M1-polarization,
elevated
levels
IL-1β
TNF-α.
Further
evaluations
knockout
mice
exhibited
superior
attenuated
present
results
suggested
that
essential
regulating
inflammatory
Suppression
has
potential
improving
medical
approaches
repair.
Advanced Healthcare Materials,
Journal Year:
2022,
Volume and Issue:
11(20)
Published: Aug. 10, 2022
Abstract
Hydrogels
with
soft
and
wet
properties
have
been
intensively
investigated
for
chronic
disease
tissue
repair.
Nevertheless,
engineering
hydrogels
containing
high
water
content
are
often
simultaneously
suffered
from
low
porous
size
water‐resistant
capacities,
leading
to
undesirable
surgery
outcomes.
Here,
a
novel
sponge‐like
macro‐porous
hydrogel
(SM‐hydrogel)
stable
structures
anti‐swelling
performances
is
developed
via
facile,
fast
yet
robust
approach
induced
by
Ti
3
C
2
MXene
additives.
The
MXene‐induced
SM‐hydrogels
(80%
content)
200–300
µm
open
macropores,
demonstrating
ideal
mass/nutrient
infiltration
capability
at
≈20‐fold
higher
water/blood‐transport
velocity
over
that
of
the
nonporous
hydrogels.
Moreover,
highly
strong
interactions
between
polymer
chains
endow
excellent
capability,
promising
equilibrium
identical
toughened
mechanical
performances.
SM‐hydrogel
versatile
functions
such
as
facilitating
mass
transport,
antibacterial
(bacterial
viability
in
(Acrylic
acid‐co‐Methacrylamide
dopamine)
copolymer‐Ti
below
25%),
reactive
oxygen
species
scavenging
capacities
(96%
ratio
120
min)
synergistically
promotes
diabetic
wound
healing
(compared
non‐porous
closure
rate
increased
39%
81%
within
7
days).
Therefore,
durable
exhibit
connective
bears
MXene,
its
great
potential
engineering.
Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
25, P. 319 - 332
Published: Feb. 11, 2023
Current
treatments
for
full-thickness
skin
injuries
are
still
unsatisfactory
due
to
the
lack
of
hierarchically
stimulated
dressings
that
can
integrate
rapid
hemostasis,
inflammation
regulation,
and
tissue
remodeling
into
one
system
instead
single-stage
boosting.
In
this
work,
a
multilayer-structured
bioactive
glass
nanopowder
(BGN@PTE)
is
developed
by
coating
poly-tannic
acid
ε-polylysine
onto
BGN
via
facile
layer-by-layer
assembly
as
an
integrative
multilevel
dressing
sequential
management
wounds.
comparison
coated
BGN,
BGN@PTE
exhibited
better
hemostatic
performance
because
its
multiple
dependent
approaches
induce
platelet
adhesion/activation,
red
blood
cells
(RBCs)
aggregation
fibrin
network
formation.
Simultaneously,
ions
from
facilitate
regulation
inflammatory
response
while
antibacterial
prevent
wound
infection,
promoting
healing
during
stage.
addition,
serve
reactive
oxygen
species
scavenger,
alleviate
oxidation
stress
in
injury,
cell
migration
angiogenesis,
promote
proliferation
stage
repair.
Therefore,
demonstrated
significantly
higher
repair
capacity
than
commercial
bioglass
Dermlin™.
This
multifunctional
potentially
valuable
may
be
expected
extend
other
wounds
therapy.
Small,
Journal Year:
2023,
Volume and Issue:
19(27)
Published: March 28, 2023
Abstract
Currently
used
wound
dressings
are
ineffective.
Hence,
there
is
a
need
to
develop
introduce
high‐performance
medicament
with
multiple
functions
including
rapid
hemostasis
and
excellent
antibacterial
activity
meet
the
growing
worldwide
demand
for
healing
products.
Here,
inspired
by
strong
adhesion
of
mussels
enzyme‐mimicking
nanometallic
biomaterials,
authors
developed
an
injectable
hydrogel
overcome
limitations
current
dressings.
The
synthesized
via
esterification
reaction
between
poly(vinyl
alcohol)
(PVA)
3,4‐dihydroxyphenylalanine
(DOPA),
followed
catechol‐metal
coordination
Cu
2+
catechol
groups
DOPA
form
PVA‐DOPA‐Cu
(PDPC)
hydrogel.
PDPC
possesses
tissue
adhesive,
antioxidative,
photothermal,
antibacterial,
hemostatic
properties.
rapidly
efficiently
stopped
bleeding
under
different
traumatic
conditions,
otherwise‐lethal
liver
injury,
high‐pressure
carotid
artery
rupture,
even
fatal
cardiac
penetration
injuries
in
animal
models.
Furthermore,
it
demonstrated
that
affected
repair
regeneration
accelerating
re‐epithelialization,
promoting
collagen
deposition,
regulating
inflammation,
contributing
vascularization.
results
show
promising
candidate
hemorrhage
control
efficient
clinical
applications.
