Sex determination mechanisms and sex control approaches in aquaculture animals

Science China Life Sciences, Journal Year: 2022, Volume and Issue: 65(6), P. 1091 - 1122

Published: May 16, 2022

Language: Английский

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Diversity and Convergence of Sex-Determination Mechanisms in Teleost Fish

Annual Review of Animal Biosciences, Journal Year: 2023, Volume and Issue: 12(1), P. 233 - 259

Published: Oct. 20, 2023

Sexual reproduction is prevalent across diverse taxa. However, sex-determination mechanisms are so that even closely related species often differ in systems. Teleost fish a taxonomic group with frequent turnovers of sex-determining and thus provides us great opportunities to investigate the molecular evolutionary underlying turnover Here, we compile recent studies on diversity fish. We demonstrate genes TGF-β signaling pathway frequently used for master (MSD) genes. MSD arise via two main mechanisms, duplication-and-transposition allelic mutations, few exceptions. also temperature influences sex determination many species, those chromosomes, higher temperatures inducing differentiation into males most cases. Finally, review theoretical models discuss what questions remain elusive.

Language: Английский

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Genomic anatomy of homozygous XX females and YY males reveals early evolutionary trajectory of sex-determining gene and sex chromosomes in Silurus fishes

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(8)

Published: Aug. 1, 2024

Abstract Sex chromosomes display remarkable diversity and variability among vertebrates. Compared with research on the X/Y Z/W chromosomes, which have long evolutionary histories in mammals birds, studies sex at early stages are limited. Here, we precisely assembled genomes of homozygous XX female YY male Lanzhou catfish (Silurus lanzhouensis) derived from an artificial gynogenetic family a self-fertilized family, respectively. Chromosome 24 (Chr24) was identified as chromosome based resequencing data. Comparative analysis X Y showed approximate 320 kb Y-specific region duplicate anti-Mullerian hormone type II receptor (amhr2y), is consistent findings 2 other Silurus species but different (Chr24 meridionalis Chr5 asotus). Deficiency amhr2y resulted male-to-female reversal, indicating that plays male-determining role S. lanzhouensis. Phylogenetic comparative genomics revealed common sex-determining gene initially translocated to Chr24 ancestor along expansion transposable elements. maintained lanzhouensis, whereas transition triggered turnover asotus. Additionally, duplication, translocation, degeneration were observed regions species. These present clear case for trajectory including origin, repeat sequence expansion, gathering region, turnover.

Language: Английский

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Multi-genome comparisons reveal gain-and-loss evolution of anti-Mullerian hormone receptor type 2 as a candidate master sex-determining gene in Percidae

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 26, 2024

The Percidae family comprises many fish species of major importance for aquaculture and fisheries. Based on three new chromosome-scale assemblies in Perca fluviatilis, schrenkii, Sander vitreus along with additional percid reference genomes, we provide an evolutionary comparative genomic analysis their sex-determination systems.

Language: Английский

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Identification of sex chromosome and sex-determining gene of southern catfish ( Silurus meridionalis ) based on XX, XY and YY genome sequencing

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 289(1971)

Published: March 16, 2022

Teleosts are important models to study sex chromosomes and sex-determining (SD) genes because they present a variety of determination systems. Here, we used Nanopore Hi-C technologies generate high-contiguity chromosome-level genome assembly YY southern catfish ( Silurus meridionalis ). The is 750.0 Mb long, with contig N50 15.96 scaffold 27.22 Mb. We also sequenced assembled an XY male size 727.2 13.69 identified candidate SD gene through comparisons our previous XX individual. By resequencing female pools, characterized 2.38 region (SDR) on Chr24. Analysis read coverage comparison the X Y chromosome sequences showed specific insertion (approx. 500 kb) in SDR which contained male-specific duplicate amhr2 (named amhr2y shared high-nucleotide identity (81.0%) coding but extremely low promotor intron regions. exclusive expression gonadal primordium loss-of-function inducing reversal confirmed role determination. Our provides new example as fish sheds light convergent evolution duplication AMH/AMHR2 pathway members underlying different lineages.

Language: Английский

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An ancient truncated duplication of the anti‐Müllerian hormone receptor type 2 gene is a potential conserved master sex determinant in the Pangasiidae catfish family

Molecular Ecology Resources, Journal Year: 2022, Volume and Issue: 22(6), P. 2411 - 2428

Published: April 16, 2022

The evolution of sex determination (SD) in teleosts is amazingly dynamic, as reflected by the variety different master sex-determining genes identified. Pangasiids are economically important catfishes South Asian countries, but little known about their SD system. Here, we generated novel genomic resources for 12 and characterized Based on a Pangasianodon hypophthalmus chromosome-scale genome assembly, identified an anti-Müllerian hormone receptor type Ⅱ gene (amhr2) duplication, which was further being sex-linked males expressed only testes. These results point to Y chromosome male-specific duplication (amhr2by) autosomal amhr2a. Sequence annotation revealed that P. Amhr2by truncated its N-terminal domain, lacking cysteine-rich extracellular part crucial ligand binding, suggesting potential route neofunctionalization. Reference-guided assembly 11 additional Pangasiids, along with sex-linkage studies, this amhr2by conserved Pangasiids. Reconstructions amhr2 phylogeny suggested arose from ancient duplication/insertion event at root Siluroidei radiation dated ~100 million years ago. Together these bring multiple lines evidence supporting finding highlights recurrent use transforming growth factor β pathway, often used recruitment teleost genes, provides another empirical case towards firther understanding dynamics systems.

Language: Английский

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Master-Key Regulators of Sex Determination in Fish and Other Vertebrates—A Review

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2468 - 2468

Published: Jan. 27, 2023

In vertebrates, mainly single genes with an allele ratio of 1:1 trigger sex-determination (SD), leading to initial equal sex-ratios. Such are designated master-key regulators (MKRs) and frequently associated DNA structural variations, such as copy-number variation null-alleles. Most MKR knowledge comes from fish, especially cichlids, which serve a genetic model for SD. We list 14 MKRs, dmrt1 has been identified in taxonomically distant species birds fish. The identification MKRs known involvement SD, amh fshr, indicates that common network drives illustrate affects estrogen/androgen equilibrium, suggesting may exert over-expression the gene thus form MKR. However, reason why certain factors constitute whereas others do not is unclear. limited number conserved suggests their heterologous sequences could be used targets future searches additional species. Sex-specific mortality, sex reversal, role temperature multigenic SD examined, claiming these phenomena often consequences artificial hybridization. discuss essentiality taxonomic authentication validate purebred origin before searches.

Language: Английский

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Identification of sex locus and a male-specific marker in blunt-snout bream (Megalobrama amblycephala) using a whole genome resequencing method

Aquaculture, Journal Year: 2024, Volume and Issue: 582, P. 740559 - 740559

Published: Jan. 8, 2024

Language: Английский

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Sex locus and sex markers identification using whole genome pool-sequencing approach in the largemouth bass (Micropterus Salmoides L.)

Aquaculture, Journal Year: 2022, Volume and Issue: 559, P. 738375 - 738375

Published: May 20, 2022

Language: Английский

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Chromosome-level genome assembly and annotation of largemouth bronze gudgeon (Coreius guichenoti)

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 15, 2025

Coreius guichenoti, mainly distributed in upstream regions of the Yangtze River China, is currently on brink extinction and listed as national secondary protected animal. In this study, we aimed to obtain chromosome-level genome C. guichenoti using PacBio Hi-C techniques. According sequencing, was successfully assembled 1100.1 Mb size, with a Contig N50 size 25.0 Mb, containing 731.0 repeats. sequencing data utilized for chromosome assembly 25 sequences were ultimately yielded, total length 1076.8 Mb. Moreover, 22,506 protein-coding genes predicted average intron 2293 bp. Evolutionary analysis divergence time prediction revealed that closely related heterodon they phylogenetically diverged from common ancestor ~20.7 million years ago (Mya), following separation Cyprinidae at 28.3 Mya. future, utilization comparative genomics research important elucidating molecular mechanisms Ichthyophthirius disease ensuring conservation biological resources.

Language: Английский

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