Animals,
Journal Year:
2025,
Volume and Issue:
15(9), P. 1283 - 1283
Published: April 30, 2025
The
use
of
environmental
DNA
(eDNA)
metabarcoding
to
analyze
fish
species
diversity
across
different
aquatic
ecosystems
is
well
documented.
Nonetheless,
there
a
gap
in
validating
eDNA
studies
on
the
and
structure
communities
coastal
ecosystems,
particularly
comparing
these
findings
with
bottom
trawl
catch
data.
In
this
study,
we
employed
explore
composition
relative
abundance
communities,
taxonomic-level
variations,
interplay
between
community
structures
factors
Yellow
Sea
compared
results
those
obtained
from
catches.
addition,
various
methods
used
estimate
distributions
taxonomic,
phylogenetic,
functional
factors.
We
found
that
detected
greater
number
(86
vs.
41),
genera
(73
37),
families
(42
25)
than
at
each
sampling
station.
provided
higher
Shannon,
Simpson,
Chao1
alpha
indices
results.
PCoA
showed
samples
could
be
more
clearly
separated
sites
Zhuanghe
(ZH)
Lianyungang
(LYG)
areas
trawling
samples.
RDA
analysis
indicated
temperature,
along
NO3-
NH4+
concentrations,
were
pivotal
shaping
geographical
patterns
as
identified
through
metabarcoding,
echoing
studies.
Furthermore,
our
suggest
barcoding
surpasses
detecting
taxonomic
phylogenetic
diversity,
uncovering
local
level.
Conclusively,
emerges
valuable
complement
trawling,
offering
multifaceted
approach
biodiversity
monitoring
not
only
boosts
efficiency
but
also
reduces
impact
ecosystems.
Molecular Ecology,
Journal Year:
2022,
Volume and Issue:
31(20), P. 5132 - 5164
Published: Aug. 16, 2022
Abstract
Vast
global
declines
of
freshwater
and
marine
fish
diversity
population
abundance
pose
serious
threats
to
both
ecosystem
sustainability
human
livelihoods.
Environmental
DNA
(eDNA)‐based
biomonitoring
provides
robust,
efficient,
cost‐effective
assessment
species
occurrences
trends
in
diverse
aquatic
environments.
Thus,
it
holds
great
potential
for
improving
conventional
surveillance
frameworks
facilitate
conservation
fisheries
management.
However,
the
many
technical
considerations
rapid
developments
underway
eDNA
arena
can
overwhelm
researchers
practitioners
new
field.
Here,
we
systematically
analysed
416
studies
summarize
research
terms
investigated
targets,
aims,
study
systems,
reviewed
applications,
rationales,
methodological
considerations,
limitations
methods
with
an
emphasis
on
research.
We
highlighted
how
technology
may
advance
our
knowledge
behaviour,
distributions,
genetics,
community
structures,
ecological
interactions.
also
synthesized
current
several
important
concerns,
including
qualitative
quantitative
power
has
recover
biodiversity
abundance,
spatial
temporal
representations
respect
its
sources.
To
applications
implementing
techniques,
recent
literature
was
summarized
generate
guidelines
effective
sampling
lentic,
lotic,
habitats.
Finally,
identified
gaps
limitations,
pointed
out
newly
emerging
avenues
eDNA.
As
optimization
standardization
improve,
should
revolutionize
monitoring
promote
management
that
transcends
geographic
boundaries.
Reviews in Aquaculture,
Journal Year:
2022,
Volume and Issue:
15(2), P. 676 - 703
Published: Oct. 22, 2022
Abstract
Aquaculture,
especially
of
non‐native
species
and
translocated
domestic
species,
is
a
greatly
encouraged
way
relieving
the
conflicts
between
food
economic
demand
resource
depletion.
We
herein
summarized
introduction
history
fish
for
aquacultural
use
in
China,
including
105
introduced
from
abroad
61
domestically
across
river
basins,
which
has
brought
great
benefits
but
high
ecological
risks.
Of
these,
one‐fourth
have
successfully
established
wild
populations
natural
waters
15%
invaded.
presented
specific
examples
seven
aquaculture
species/taxa
three
aquarium
to
explain
their
outcomes.
The
notable
benefits,
complex
composition
strain
selection
tilapias,
carps
sturgeons
may
together
facilitate
invasion,
result
diversity
decline,
genetic
pollution,
loss
ecosystem
service.
specifically
reviewed
invasion
cases
lakes
reservoirs
found
that
western
China
made
by
major
hydroprojects
are
hotspots
this
led
disappearance
endemic
changes
original
faunal
composition.
escaped
via
changed
biogeography,
resulting
assemblage
uniqueness
causing
homogeneity.
achieved
significant
progress
completing
Aichi
Targets
improving
legal
system
strengthening
conservational
actions
on
controlling
species.
Further
actions,
risk
assessment
management
expected
healthy
outlook
industry
China.
Environment International,
Journal Year:
2024,
Volume and Issue:
187, P. 108706 - 108706
Published: April 26, 2024
Environmental
DNA
(eDNA)
technology
has
revolutionized
biomonitoring,
but
challenges
remain
regarding
water
sample
processing.
The
passive
eDNA
sampler
(PEDS)
represents
a
viable
alternative
to
active,
filtration-based
enrichment
methods,
the
effectiveness
of
PEDS
for
surveying
biodiverse
and
complex
natural
bodies
is
unknown.
Here,
we
collected
using
filtration
glass
fiber
filter-based
(submerged
in
1
d)
from
27
sites
along
final
reach
Yangtze
River
coast
Yellow
Sea,
followed
by
metabarcoding
analysis
fish
biodiversity
quantitative
PCR
(qPCR)
critically
endangered
aquatic
mammal,
finless
porpoise.
We
ultimately
detected
98
species
via
metabarcoding.
Both
sampling
methods
captured
comparable
local
richness
revealed
largely
similar
spatial
variation
assemblages
community
partitions
between
river
sea
sites.
Notably,
porpoise
was
only
at
five
Also,
species-specific
qPCR
that
more
(7
vs.
2),
greater
quantities,
with
higher
detection
probability
(0.803
0.407)
than
did
filtration.
Our
results
demonstrate
capacity
biodiversity,
support
continuous
collection
can
be
effective
instantaneous
capturing
low
abundance
ephemeral
waters.
Thus,
approach
facilitate
efficient
convenient
eDNA-based
surveillance
rare
detection.
Environmental Science & Technology,
Journal Year:
2022,
Volume and Issue:
56(15), P. 10798 - 10807
Published: July 20, 2022
Environmental
DNA
(eDNA)
technology
has
revolutionized
biomonitoring
in
recent
years;
however,
eDNA
collection
from
aquatic
environments
generally
relies
on
the
time-consuming
and
equipment-dependent
process
of
water
filtration.
Passive
sampling
deploys
sorbent
materials
to
capture
water,
circumventing
many
problems
associated
with
active
filtration;
yet,
very
few
candidate
have
been
systematically
evaluated
for
this
purpose.
Here,
we
ability
12
different
types
common
loose
sorbents
filter
membranes
laboratory
field
experiments
compared
conventional
Glass
fiber
filters
(GF)
outperformed
all
other
respect
their
quantitative
capacity
recover
amphibian
eDNA,
yield
increasing
linearly
submersion
time
up
72
h.
Furthermore,
GF
rapidly
(within
0.5
h)
captured
71%
total
fish
species
a
lake,
addition
detecting
entire
community
by
8
h,
as
assessed
metabarcoding
analysis.
Our
results
demonstrate
that
could
passively
aqueous
similar
or
greater
efficiency
than
methods,
thus
paving
way
convenient,
effective,
eco-friendly
environments.
