Galectin functions in cancer-associated inflammation and thrombosis

Frontiers in Cardiovascular Medicine, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 17, 2023

Galectins are carbohydrate-binding proteins that regulate many cellular functions including proliferation, adhesion, migration, and phagocytosis. Increasing experimental clinical evidence indicates galectins influence steps of cancer development by inducing the recruitment immune cells to inflammatory sites modulating effector function neutrophils, monocytes, lymphocytes. Recent studies described different isoforms can induce platelet aggregation, granule release through interaction with platelet-specific glycoproteins integrins. Patients and/or deep-venous thrombosis have increased levels in vasculature, suggesting these could be important contributors cancer-associated inflammation thrombosis. In this review, we summarize pathological role thrombotic events, influencing tumor progression metastasis. We also discuss potential anti-cancer therapies targeting context

Language: Английский

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Investigating the effects of PTEN mutations on cGAS-STING pathway in glioblastoma tumours

Journal of Neuro-Oncology, Journal Year: 2024, Volume and Issue: 166(2), P. 283 - 292

Published: Jan. 1, 2024

Abstract Background PTEN is a tumour suppressor gene and well-known for being frequently mutated in several cancer types. Loss of immunogenicity can also be attributed to loss, because its role establishing the microenvironment. Therefore, this study aimed represent link between cGAS-STING activity, key mediator inflammation, samples glioblastoma patients. Methods Tumour 36 patients were collected. After DNA isolation, all coding regions sequenced analysed. expression status was evaluated by qRT-PCR, western blot, immunohistochemical methods. Interferon-stimulated expressions, cGAMP CD8 infiltration, Granzyme B levels determined especially evaluation activity immunogenicity. Results Mutant had significantly lower expression, both at mRNA protein levels. Decreased STING, IRF3, NF-KB1 , RELA expressions found with mutant . Immunohistochemistry staining displayed expressional loss 38.1% Besides, considerably amounts IFNB IFIT2 expressions. Furthermore, cGAMP, reduced group. Conclusion This reveals immunosuppressive effects tumours via pathway. determining great importance, like situations when considering treatment immunotherapeutic agents.

Language: Английский

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VEGFA contributes to tumor property of glioblastoma cells by promoting differentiation of myeloid-derived suppressor cells

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 22, 2024

Glioblastoma (GBM) is a malignant astrocytic tumor and its progression involves the regulation of vascular endothelial growth factor-A (VEGFA). However, mechanism VEGFA in regulating GBM remains unclear. mRNA expression was analyzed by quantitative real-time polymerase chain reaction. Protein VEGFA, cluster differentiation 9 (CD9), CD81, transforming factor-β1 (TGF-β1) detected western blotting assay. Flow cytometry assay conducted to assess cell proliferation, apoptosis myeloid-derived suppressor (MDSC) differentiation. TUNEL detection kit utilized analyze tumors. Angiogenic capacity investigated tube formation Transwell used migration invasion. The effect on determined xenograft mouse model Immunohistochemistry positive rate tissues. TGF-β1 level enzyme-linked immunosorbent upregulated tissues, cells, exosomes from patients cells. silencing led decreased formation, invasion increased apoptosis. Moreover, knockdown also delayed formation. promoted MDSC secretion MDSCs being packaged into exosomes. In addition, displayed similar effects with phenotypes, attenuated knockdown-induced secreting A172 U251 contributed property cells promoting MDSCs, providing potential targets for treatment. tissues cells; inhibited induced exosomes; producing

Language: Английский

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Sophocarpine inhibits the proliferation and induces apoptosis of glioblastoma cells through regulating the miR-21/PTEN/PI3K/AKT axis

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 8, 2025

Sophocarpine (SC) has been reported to suppress tumorigenesis. But the effect of SC on glioblastoma (GBM) is unknown. This study explored anti-proliferation and pro-apoptosis effects GBM cells molecular mechanism. Different concentrations were used treat human astrocyte NHA lines LN229 SF539. CCK-8 was applied analyze cell toxicity proliferation. qRT-PCR western blot measure RNA protein expressions, respectively. Cell cycle apoptosis determined by flow cytometry assay. The results indicated that inhibited proliferation induced SF539 in a dose-dependent manner. arrest G0/G1 phase increased after treatment. Moreover, downregulated miR-21 expression upregulated PTEN cells. Overexpression partly abrogated cells, while exogenous partially eliminated pro-proliferation anti-apoptosis Furthermore, treatment decreased levels PI3K/AKT pathway-related p-PI3K, p-AKT PIP3 pathway activator 740Y-P reversed reduced enhanced SC-treated Significantly, we verified suppressed via inhibiting it not entirely dependent upregulation PTEN. Consequently, potential mechanism induction verified, which might provide new method for

Language: Английский

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A novel galectin with triple carbohydrate recognition domain in the parotoid secretion of Rhinella diptycha

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141586 - 141586

Published: Feb. 27, 2025

Language: Английский

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