Isolation of a methyl-reducing methanogen outside the Euryarchaeota DOI Creative Commons
Lei Cheng, Kejia Wu, Lei Zhou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 14, 2023

Abstract Methanogenic archaea are main contributors to methane emissions, and thus play a crucial role in carbon cycling global warming. Until recently, methanogens were confined the phylum Euryarchaeota, but metagenomic studies revealed presence of genes encoding methyl coenzyme M reductase complex other archaeal clades, thereby opening up premise that methanogenesis is taxonomically more widespread. Nevertheless, laboratory cultivation these non-Euryarchaeal was missing allow study their physiology corroborate potential methanogenic capability. Here we describe thermophilic co-culture from an oil field, containing single archaeon (strain LWZ-6) belonging proposed order Candidatus Verstraetearchaeia, together with H2-producing Acetomicrobium sp. CY-2. Strain LWZ-6, for which propose name Verstraetearchaeum methanopetracarbonis, H2-dependent methylotrophic methanogen. Although previous speculated on fermentative Verstraetearchaeial methanogens, strain LWZ-6 does not ferment sugars, peptides, amino acids. Its energy metabolism linked methanogenesis, methanol monomethylamine as electron acceptors H2 donor. Comparative (meta)genome analysis shared trait among Verstraetearchaeia. Our findings diversity expands beyond classical Euryarchaeota change our current conception cycle.

Language: Английский

Isolation of a methyl-reducing methanogen outside the Euryarchaeota DOI
Kejia Wu, Lei Zhou,

Guillaume Tahon

et al.

Nature, Journal Year: 2024, Volume and Issue: 632(8027), P. 1124 - 1130

Published: July 24, 2024

Language: Английский

Citations

18
Unveiling the unique role of iron in the metabolism of methanogens: A review DOI
Yuxin Ma, Ying Qu,

Xiangwu Yao

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 250, P. 118495 - 118495

Published: Feb. 15, 2024

Language: Английский

Citations

10
Methane formation driven by light and heat prior to the origin of life and beyond DOI Creative Commons
Leonard Ernst, Uladzimir Barayeu, Jonas Hädeler

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 1, 2023

Methane is a potent greenhouse gas, which likely enabled the evolution of life by keeping early Earth warm. Here, we demonstrate routes towards abiotic methane and ethane formation under early-earth conditions from methylated sulfur nitrogen compounds with prebiotic origin. These are demethylated in Fenton reactions governed ferrous iron reactive oxygen species (ROS) produced light heat aqueous environments. After emergence life, this phenomenon would have greatly intensified anoxic Archean providing substrates. This ROS-driven chemistry can occur delocalized serpentinization across Earth's humid realm thereby substantially differs previously suggested that spatially restricted. report driven release might shaped chemical atmosphere prior to origin beyond.

Language: Английский

Citations

22
Candidatus List. Lists of names of prokaryotic Candidatus phyla DOI Open Access
Aharon Oren, Markus Göker

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2023, Volume and Issue: 73(5)

Published: May 9, 2023

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher we support invest in the microbiology community, to benefit of everyone. This supports our principal goal develop, expand strengthen networks available members so that they can generate new knowledge about microbes ensure it is shared with other communities.

Language: Английский

Citations

17
Methylotrophic methanogenesis in the Archaeoglobi revealed by cultivation of Ca. Methanoglobus hypatiae from a Yellowstone hot spring DOI Creative Commons
Mackenzie M. Lynes, Zackary J. Jay, Anthony J. Kohtz

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Over the past decade, environmental metagenomics and polymerase chain reaction-based marker gene surveys have revealed that several lineages beyond just a few well-established groups within Euryarchaeota superphylum harbor genetic potential for methanogenesis. One of these are Archaeoglobi, class thermophilic long been considered to live non-methanogenic lifestyles. Here, we enriched Candidatus Methanoglobus hypatiae, methanogen affiliated with family Archaeoglobaceae, from hot spring in Yellowstone National Park. The enrichment is sediment-free, grows at 64–70°C pH 7.8, produces methane mono-, di-, tri-methylamine. Ca. M. hypatiae represented by 1.62 Mb metagenome-assembled genome an estimated completeness 100% accounts up 67% cells culture according fluorescence situ hybridization. Via genome-resolved metatranscriptomics stable isotope tracing, demonstrate expresses methylotrophic methanogenesis energy-conserving pathways reducing monomethylamine methane. detection Archaeoglobi populations related 36 geochemically diverse geothermal sites Park, as through examination previously published amplicon datasets, implies underestimated contribution anaerobic carbon cycling extreme ecosystems.

Language: Английский

Citations

6
A non-methanogenic archaeon within the order Methanocellales DOI Creative Commons
Shino Suzuki, Shun’ichi Ishii, Grayson L. Chadwick

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 13, 2024

Abstract Serpentinization, a geochemical process found on modern and ancient Earth, provides an ultra-reducing environment that can support microbial methanogenesis acetogenesis. Several groups of archaea, such as the order Methanocellales , are characterized by their ability to produce methane. Here, we generate metagenomic sequences from serpentinized springs in The Cedars, California, construct circularized metagenome-assembled genome archaeon, termed Met12, lacks essential genes. includes genes for acetyl-CoA pathway, but encoding enzymes methyl-coenzyme M reductase, heterodisulfide reductases hydrogenases. In situ transcriptomic analyses reveal high expression multi-heme c- type cytochrome, heterologous this protein model bacterium demonstrates it is capable accepting electrons. Our results suggest within not methanogen CO 2 -reducing, electron-fueled acetogen without electron bifurcation.

Language: Английский

Citations

6
Asgard archaea modulate potential methanogenesis substrates in wetland soil DOI Creative Commons
Luis E. Valentin-Alvarado, Kathryn E. Appler, Valerie De Anda

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 31, 2024

Abstract The roles of Asgard archaea in eukaryogenesis and marine biogeochemical cycles are well studied, yet their contributions soil ecosystems remain unknown. Of particular interest archaeal to methane cycling wetland soils. To investigate this, we reconstructed two complete genomes for soil-associated Atabeyarchaeia, a new lineage, genome Freyarchaeia, predicted metabolism situ. Metatranscriptomics reveals expression genes [NiFe]-hydrogenases, pyruvate oxidation carbon fixation via the Wood-Ljungdahl pathway. Also expressed encoding enzymes amino acid metabolism, anaerobic aldehyde oxidation, hydrogen peroxide detoxification carbohydrate breakdown acetate formate. Overall, include non-methanogenic acetogens, highlighting potential role terrestrial environments.

Language: Английский

Citations

6
Reconstructing Early Microbial Life DOI
Betül Kaçar

Annual Review of Microbiology, Journal Year: 2024, Volume and Issue: 78(1), P. 463 - 492

Published: Aug. 21, 2024

For more than 3.5 billion years, life experienced dramatic environmental extremes on Earth. These include shifts from oxygen-less to overoxygenated atmospheres and cycling between hothouse conditions global glaciations. Meanwhile, an ecological revolution took place. Earth evolved one dominated by microbial containing the plants animals that are most familiar today. Many key cellular features early in history of life, collectively defining nature our biosphere underpinning human survival. Recent advances molecular biology bioinformatics have greatly improved understanding evolution across deep time. However, incorporation genetics, population biology, evolutionary approaches into study Precambrian biota remains a significant challenge. This review synthesizes current knowledge with emphasis ancient metabolisms. It also outlines foundations emerging interdisciplinary area integrates microbiology, paleobiology, synthetic reconstruct biological innovations.

Language: Английский

Citations

6
Evidence for nontraditional mcr -containing archaea contributing to biological methanogenesis in geothermal springs DOI Creative Commons
Jiajia Wang,

Yan-Ni Qu,

Paul N. Evans

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(26)

Published: June 28, 2023

Recent discoveries of methyl-coenzyme M reductase-encoding genes (mcr) in uncultured archaea beyond traditional euryarchaeotal methanogens have reshaped our view methanogenesis. However, whether any these nontraditional perform methanogenesis remains elusive. Here, we report field and microcosm experiments based on 13C-tracer labeling genome-resolved metagenomics metatranscriptomics, revealing that are predominant active methane producers two geothermal springs. Archaeoglobales performed from methanol may exhibit adaptability using methylotrophic hydrogenotrophic pathways temperature/substrate availability. A five-year survey found Candidatus Nezhaarchaeota to be the mcr-containing inhabiting springs; genomic inference mcr expression under methanogenic conditions strongly suggested this lineage mediated situ. Methanogenesis was temperature-sensitive , with a preference for over when incubation temperatures increased 65° 75°C. This study demonstrates an anoxic ecosystem wherein is primarily driven by known methanogens, highlighting diverse as previously unrecognized sources.

Language: Английский

Citations

16
Biosynthesis of GMGT lipids by a radical SAM enzyme associated with anaerobic archaea and oxygen-deficient environments DOI Creative Commons
Yanan Li, Ting Yu, Xi Feng

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 19, 2024

Abstract Archaea possess characteristic membrane-spanning lipids that are thought to contribute the adaptation extreme environments. However, biosynthesis of these is poorly understood. Here, we identify a radical S -adenosyl-L-methionine (SAM) enzyme synthesizes glycerol monoalkyl tetraethers (GMGTs). The enzyme, which name GMGT synthase (Gms), catalyzes formation C(sp 3 )–C(sp ) linkage between two isoprenoid chains dialkyl (GDGTs). This conclusion supported by heterologous expression gene gms from GMGT-producing species in methanogen, as well demonstration vitro activity using purified Gms enzyme. Additionally, show genes encoding putative homologs present obligate anaerobic archaea and metagenomes obtained oxygen-deficient environments, appear be absent oxic settings.

Language: Английский

Citations

5