A disease resistance protein triggers oligomerization of its NLR helper into a hexameric resistosome to mediate innate immunity DOI Creative Commons
Jogi Madhuprakash, AmirAli Toghani, Mauricio P. Contreras

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Abstract NRCs are essential helper NLR (nucleotide-binding domain and leucine-rich repeat) proteins that execute the immune response triggered by disease resistance proteins, also known as sensor NLRs. The structure of resting state NbNRC2 was recently revealed to be a homodimer. However, sensor-activated has not yet been elucidated. In this study, we used cryo-EM determine NbNRC2, which forms hexameric inflammasome-like resistosome. To confirm functional significance hexamer, mutagenized interfaces involved in oligomerization found mutations three nucleotide-binding interface residues abolish signalling. Comparative structural analyses between homodimer homohexamer significant rearrangements before after activation, providing insights into activation mechanisms. Furthermore, comparisons hexamer previously reported CC-NLR pentameric assemblies features allow for integration an additional protomer. We assess released AlphaFold 3 prediction activated oligomers. This allows high-confidence modelling N-terminal α 1-helices other CC-NLRs, region proven difficult fully resolve using approaches. Overall, our work sheds light on biochemical mechanisms underpinning expands understanding diversity.

Language: Английский

Plant immune signaling network mediated by helper NLRs DOI

Yihan Gong,

Lei Tian, Ilias Kontos

et al.

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 73, P. 102354 - 102354

Published: March 30, 2023

Language: Английский

Citations

20
Genetics of Resistance to Leaf Rust in Wheat: An Overview in a Genome-Wide Level DOI Open Access

Xiaopeng Ren,

Chuyuan Wang, Zhuang Ren

et al.

Sustainability, Journal Year: 2023, Volume and Issue: 15(4), P. 3247 - 3247

Published: Feb. 10, 2023

Due to the global warming and dynamic changes in pathogenic virulence, leaf rust caused by Puccinia triticina has greatly expanded its epidermic region become a severe threat wheat production. Genetic bases of resistance mainly rely on (Lr) gene or quantitative trait locus (QLr). Although these genetic loci have been insensitively studied during last two decades, an updated overview Lr/QLr genome-wide level is urgently needed. This review summarized recent progresses studies rust. Wheat germplasms with great potentials for improvement were highlighted. Key information about carrying was summarized. A chromosome distribution map all generated based released reference genome. In conclusion, this provided valuable sources both breeders researchers understand genetics wheat.

Language: Английский

Citations

19
Comparative Analysis of the Avirulence Effectors Produced by the Fungal Stem Rust Pathogen of Wheat DOI Creative Commons

Jibril Lubega,

Melania Figueroa, Peter N. Dodds

et al.

Molecular Plant-Microbe Interactions, Journal Year: 2024, Volume and Issue: 37(3), P. 171 - 178

Published: Jan. 3, 2024

Crops are constantly exposed to pathogenic microbes. Rust fungi examples of these harmful microorganisms, which have a major economic impact on wheat production. To protect themselves from pathogens like rust fungi, plants employ multilayered immune system that includes immunoreceptors encoded by resistance genes. Significant efforts led the isolation numerous genes against in cereals, especially wheat. However, evolution virulence hinders durability as strategy for crop protection. other biotrophic pathogens, secrete an arsenal effectors facilitate infection, and molecules plant target pathogen recognition mounting defense responses. When recognized, effector proteins referred avirulence (Avr) effectors. Despite many predicted only five

Language: Английский

Citations

7
MAPK Cascades in Plant Microbiota Structure and Functioning DOI
Thijs Van Gerrewey, Hoo Sun Chung

The Journal of Microbiology, Journal Year: 2024, Volume and Issue: 62(3), P. 231 - 248

Published: March 1, 2024

Language: Английский

Citations

7
A disease resistance protein triggers oligomerization of its NLR helper into a hexameric resistosome to mediate innate immunity DOI Creative Commons
Jogi Madhuprakash, AmirAli Toghani, Mauricio P. Contreras

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Abstract NRCs are essential helper NLR (nucleotide-binding domain and leucine-rich repeat) proteins that execute the immune response triggered by disease resistance proteins, also known as sensor NLRs. The structure of resting state NbNRC2 was recently revealed to be a homodimer. However, sensor-activated has not yet been elucidated. In this study, we used cryo-EM determine NbNRC2, which forms hexameric inflammasome-like resistosome. To confirm functional significance hexamer, mutagenized interfaces involved in oligomerization found mutations three nucleotide-binding interface residues abolish signalling. Comparative structural analyses between homodimer homohexamer significant rearrangements before after activation, providing insights into activation mechanisms. Furthermore, comparisons hexamer previously reported CC-NLR pentameric assemblies features allow for integration an additional protomer. We assess released AlphaFold 3 prediction activated oligomers. This allows high-confidence modelling N-terminal α 1-helices other CC-NLRs, region proven difficult fully resolve using approaches. Overall, our work sheds light on biochemical mechanisms underpinning expands understanding diversity.

Language: Английский

Citations

7