Structure of an insect gustatory receptor DOI Creative Commons
Heather M. Frank, Sanket Walujkar, Richard M. Walsh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 19, 2023

SUMMARY Gustatory Receptors (GRs) are critical for insect chemosensation and potential targets controlling pests disease vectors. However, GR structures have not been experimentally determined. We present of Bombyx mori Gr9 (BmGr9), a fructose-gated cation channel, in agonist-free fructose-bound states. BmGr9 forms tetramer similar to distantly related Olfactory (ORs). Upon fructose binding, BmGr9’s ion channel gate opens through helix S7b movements. In contrast ORs, BmGR9’s ligand-binding pocket, shaped by kinked S4 shorter extracellular S3-S4 loop, is larger solvent accessible both Also unlike binding involves S5 pocket lined with aromatic polar residues. Structure-based sequence alignments reveal distinct patterns residue conservation subfamilies associated ligand classes. These data provide insight into the molecular basis specificity function.

Language: Английский

Structural basis of ligand specificity and channel activation in an insect gustatory receptor DOI Creative Commons
Heather M. Frank, Sanket Walujkar, Richard M. Walsh

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(4), P. 114035 - 114035

Published: April 1, 2024

Gustatory receptors (GRs) are critical for insect chemosensation and potential targets controlling pests disease vectors, making their structural investigation a vital step toward such applications. We present structures of Bombyx mori Gr9 (BmGr9), fructose-gated cation channel, in agonist-free fructose-bound states. BmGr9 forms tetramer similar to distantly related odorant (ORs). Upon fructose binding, BmGr9's channel gate opens through helix S7b movements. In contrast ORs, ligand-binding pocket, shaped by kinked S4 shorter extracellular S3-S4 loop, is larger solvent accessible both Also, unlike binding involves S5 pocket lined with aromatic polar residues. Structure-based sequence alignments reveal distinct patterns residue conservation GR subfamilies associated different ligand classes. These data provide insight into the molecular basis specificity function.

Language: Английский

Citations

16
A sexually transmitted sugar orchestrates reproductive responses to nutritional stress DOI Creative Commons
Seong-Jin Kim, Kang‐Min Lee,

Si Hyung Park

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 1, 2024

Language: Английский

Citations

4
A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster DOI Creative Commons
Yuto Yoshinari, T. Nishimura, Taishi Yoshii

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to deficiency are well-studied, there a significant gap in our understanding of how organisms adaptively suppress intake. In the present study, utilizing fruit fly, Drosophila melanogaster, we discover that peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells response high-protein diet (HPD), vital suppressing overconsumption protein. Gut-derived CCHa1 received small subset enteric neurons produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment CCHa1-mediated gut-enteric neuronal axis results ammonia accumulation and shortened lifespan under HPD conditions. Collectively, findings unravel crosstalk gut pathways orchestrate physiological responses prevent adapt dietary overload.

Language: Английский

Citations

4
Reciprocal interactions between neuropeptide F and RYamide regulate host attraction in the mosquito Aedes aegypti DOI Creative Commons
Xiaoyi Dou, Kangkang Chen, Mark R. Brown

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(28)

Published: July 1, 2024

Female mosquitoes produce eggs in gonadotrophic cycles that are divided between a previtellogenic and vitellogenic phase. Previtellogenic females consume water sugar sources like nectar while also being attracted to hosts for blood feeding. Consumption of meal activates the phase, which produces mature suppresses host attraction. In this study, we tested hypothesis neuropeptide Y-like hormones differentially modulate attraction behavior mosquito Aedes aegypti . A series experiments collectively indicated enteroendocrine cells (EECs) posterior midgut release F (NPF) into hemolymph during phase stimulates humans biting behavior. meal, primarily consists protein by dry weight, down-regulated NPF EECs until developed, was associated with decline titer. depletion depended on digestion but not EEC loss. Other showed neurons terminal ganglion extend axons RYamide, evidence increased secretion circulation after meal. Injection RYamide-1 -2 suppressed attraction, coinjection RYamides or without short NPF-2 inhibited activity NPF. Overall, our results identify RYamide as gut-associated A. link shifts diet sequential cycles.

Language: Английский

Citations

2
A sexually transmitted sugar orchestrates reproductive responses to nutritional stress DOI Creative Commons
Young‐Joon Kim, Seong-Jin Kim, Kang‐Min Lee

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Abstract Seminal fluid is rich in sugars, but their role beyond supporting sperm motility unknown. In this study, we found Drosophila melanogaster males transfer a substantial amount of phospho-galactoside to females during mating, only half as much when undernourished. This seminal substance, which named venerose, induces an increase germline stem cells (GSCs) and promotes storage females, especially undernourished ones. Venerose enters the hemolymph directly activates nutrient-sensing Dh44+ neurons brain. Food deprivation directs secrete more neuropeptide Dh44 response infused venerose. The secreted then enhances local niche signal, stimulating GSC proliferation. It also extends retention ejaculate by resulting greater venerose absorption increased storage. uncovered sugar-like substance produced that coordinates reproductive responses nutritional challenges females.

Language: Английский

Citations

1
Nutritional Adaptation and Microbes: Insights From Drosophila DOI
Yukako Hattori

ZOOLOGICAL SCIENCE, Journal Year: 2024, Volume and Issue: 42(1)

Published: Dec. 20, 2024

Life-history traits such as growth, reproduction, and lifespan in animals are shaped by both genetic environmental factors, with nutrition being one of the most important factors. However, it remains unclear how to what extent changes nutritional environment affect molecular mechanisms they employ adapt these varying conditions. In recent years, fruit fly Drosophila melanogaster related species have been developed model systems for studying effects microbes on at level. This review summarizes findings adaptation species, focusing nutrition-dependent neuronal developmental mechanisms, carbohydrate-responsive that generate differences adaptabilities among animal-associated support host growth.

Language: Английский

Citations

1
Single‐cell transcriptomes provide insights into expansion of glial cells in Bombyx mori DOI
X Y Liu, Zhongjie Zhang, Bo Hu

et al.

Insect Science, Journal Year: 2023, Volume and Issue: 31(4), P. 1041 - 1054

Published: Nov. 20, 2023

Abstract The diversity of cell types in the brain and how these change during different developmental stages, remains largely unknown. life cycle insects is short goes through 4 distinct stages including embryonic, larval, pupal, adult stages. During postembryonic life, larval transforms into a mature version after metamorphosis. silkworm, Bombyx mori , lepidopteran model insect. Here, we characterized repertoire B. by obtaining 50 708 single‐cell transcriptomes. Seventeen 12 clusters from brains were assigned based on marker genes, respectively. Identified include Kenyon cells, optic lobe monoaminergic neurons, surface glia, astrocyte glia. We further assessed type compositions brains. found that transition larva to resulted great expansion glial cells. accounted for 49.8% midbrain Compared flies ants, mushroom body kenyon insufficient which accounts 5.4% 3.6% brains, Analysis neuropeptide expression showed abundance specificity varied among individual neuropeptides. Intriguingly, ion transport peptide was specifically expressed cells atlas dataset provides an important resource explore diversity, neural circuits genetic profiles.

Language: Английский

Citations

3
Mating-induced increase of kynurenine in Drosophila ovary enhances starvation resistance of progeny DOI Creative Commons
N. Hikawa, Soshiro Kashio, Masayuki Miura

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(3), P. 105663 - 105663

Published: Jan. 21, 2024

Language: Английский

Citations

0
A high-protein diet-responsive gut hormone regulates behavioural and metabolic optimization in Drosophila melanogaster DOI Creative Commons
Ryusuke Niwa, Yuto Yoshinari, T. Nishimura

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 15, 2024

Abstract Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to deficiency are well-studied, there a significant gap in our understanding of how organisms adaptively suppress intake. In the present study, utilizing fruit fly, Drosophila melanogaster , we discovered that peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells response high-protein diet (HPD), vital suppressing overconsumption protein. Gut-derived CCHa1 received small subset enteric neurons produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment CCHa1-mediated gut-enteric neuron axis results ammonia accumulation and shortened lifespan under HPD conditions. Collectively, findings unravel crosstalk gut neuronal pathways orchestrate physiological responses prevent adapt dietary overload.

Language: Английский

Citations

0
Large-scale omics analyses of nutrition-responsive mechanisms of female germline stem cell proliferation and maintenance in Drosophila melanogaster DOI
Ryosuke Hayashi, Ryusuke Niwa

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 67, P. 101296 - 101296

Published: Nov. 8, 2024

Language: Английский

Citations

0