Structure of an insect gustatory receptor DOI Creative Commons
Heather M. Frank, Sanket Walujkar, Richard M. Walsh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 19, 2023

SUMMARY Gustatory Receptors (GRs) are critical for insect chemosensation and potential targets controlling pests disease vectors. However, GR structures have not been experimentally determined. We present of Bombyx mori Gr9 (BmGr9), a fructose-gated cation channel, in agonist-free fructose-bound states. BmGr9 forms tetramer similar to distantly related Olfactory (ORs). Upon fructose binding, BmGr9’s ion channel gate opens through helix S7b movements. In contrast ORs, BmGR9’s ligand-binding pocket, shaped by kinked S4 shorter extracellular S3-S4 loop, is larger solvent accessible both Also unlike binding involves S5 pocket lined with aromatic polar residues. Structure-based sequence alignments reveal distinct patterns residue conservation subfamilies associated ligand classes. These data provide insight into the molecular basis specificity function.

Language: Английский

Structure of an insect gustatory receptor DOI Creative Commons
Heather M. Frank, Sanket Walujkar, Richard M. Walsh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 19, 2023

SUMMARY Gustatory Receptors (GRs) are critical for insect chemosensation and potential targets controlling pests disease vectors. However, GR structures have not been experimentally determined. We present of Bombyx mori Gr9 (BmGr9), a fructose-gated cation channel, in agonist-free fructose-bound states. BmGr9 forms tetramer similar to distantly related Olfactory (ORs). Upon fructose binding, BmGr9’s ion channel gate opens through helix S7b movements. In contrast ORs, BmGR9’s ligand-binding pocket, shaped by kinked S4 shorter extracellular S3-S4 loop, is larger solvent accessible both Also unlike binding involves S5 pocket lined with aromatic polar residues. Structure-based sequence alignments reveal distinct patterns residue conservation subfamilies associated ligand classes. These data provide insight into the molecular basis specificity function.

Language: Английский

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