Journal of Tissue Engineering,
Journal Year:
2025,
Volume and Issue:
16
Published: April 1, 2025
Organoids,
as
3D
in
vitro
models
derived
from
stem
cells,
have
unparalleled
advantages
over
traditional
cell
and
animal
for
studying
organogenesis,
disease
mechanisms,
drug
screening,
personalized
diagnosis
treatment.
Despite
the
tremendous
progress
made
organoid
technology,
translational
application
of
organoids
still
presents
enormous
challenges
due
to
complex
structure
function
human
organs.
In
this
review,
limitations
technologies
are
first
described.
Next,
we
explore
ways
address
many
cultures
by
engineering
various
dimensions
systems.
Finally,
discuss
future
directions
field,
including
potential
roles
simulated
microphysiology
system
We
hope
that
review
inspires
research
into
system.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
36(9)
Published: Nov. 22, 2023
Human
intestinal
organoids
(HIOs)
derived
from
pluripotent
stem
cells
or
adult
cell
biopsies
represent
a
powerful
platform
to
study
human
development,
drug
testing,
and
disease
modeling
in
vitro,
serve
as
source
for
tissue
regeneration
therapeutic
advances
vivo.
Synthetic
hydrogels
can
be
engineered
analogs
of
the
extracellular
matrix
support
HIO
growth
differentiation.
These
allow
tuning
mechanical
biochemical
properties
matrix,
offering
an
advantage
over
biologically
such
Matrigel.
have
been
used
repopulating
transplantable
grafts
vivo
delivery
injured
site.
The
use
synthetic
vitro
culture
is
expected
significantly
increase
relevance
screening,
modeling,
applications.
Aggregate,
Journal Year:
2024,
Volume and Issue:
5(2)
Published: Jan. 4, 2024
Abstract
Organoids
have
emerged
as
a
powerful
platform
for
studying
complex
biological
processes
and
diseases
in
vitro.
However,
most
studies
focused
on
individual
organoids,
overlooking
the
inter‐organ
interactions
vivo
limiting
physiological
relevance
of
models.
To
address
this
limitation,
development
multi‐organoid
system
has
gained
considerable
attention.
This
aims
to
recapitulate
communication
enable
study
processes.
review
provides
comprehensive
overview
recent
advancements
organoid
engineering
emerging
strategies
constructing
system.
First,
we
highlight
critical
mechanical,
structural,
biochemical
factors
involved
designing
suitable
materials
growth
different
organoids.
Additionally,
discuss
incorporation
dynamic
culture
environments
enhance
inter‐organoid
communication.
Furthermore,
explore
techniques
manipulating
morphogenesis
spatial
positioning
organoids
establish
effective
networks.
We
summarize
achievements
utilizing
vitro,
including
assembloids
microfluidic
platforms.
Lastly,
existing
challenges
opportunities
developing
from
its
technical
bottlenecks
scalability
applications
toward
human
diseases.
Cell Reports Physical Science,
Journal Year:
2025,
Volume and Issue:
unknown, P. 102366 - 102366
Published: Jan. 1, 2025
SummaryPhotochemistry
has
emerged
as
a
powerful
tool
for
manipulating
the
dynamic
and
heterogeneous
properties
of
hydrogel
microenvironments
in
tissue
engineering
mechanobiology.
Enhanced
spatiotemporal
control
over
mechanical
can
be
achieved
by
incorporating
an
array
photosensitive
functional
groups
within
polymer
networks
controlling
photokinetics
through
light
illumination.
This
review
explores
how
light-stimulated
photocleavage,
addition,
exchange,
isomerization
reactions
are
utilized
to
generate
hydrogels
that
soften
stiffen
situ,
enabling
precise
cell
functionality
tissue-engineered
constructs.
Advancements
design
biofabrication
platforms
have
enhanced
these
permit
local
modulation
larger
discussed.
The
applications
understanding
cellular
mechanosensation,
investigating
fibrotic
disease,
directing
stem
differentiation
formation
examined.
While
significant
progress
been
made
toward
on-demand
switch
between
multiple
conditions,
this
highlights
need
materials
undergo
progressive
stiffening.
also
emerging
photochemistry
intracellular
environments
its
potential
integration
with
advanced
force
spectroscopy
techniques
live-cell
mechanobiology
studies.
Overall,
using
stimulus
tunability,
reaction
kinetics,
compared
other
stimuli-driven
systems,
opening
new
avenues
biomimetic
material
mechanobiological
investigations.Graphical
abstract
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: March 4, 2025
Small
intestinal
neuroendocrine
tumors
(siNETs)
are
rare
bowel
arising
from
malignant
enteroendocrine
cells,
which
normally
regulate
digestion
throughout
the
intestine.
Though
infrequent,
their
incidence
is
rising
through
better
diagnosis,
fostering
research
into
origin
and
treatment.
To
date,
siNETs
considered
to
be
a
single
entity
clinically
treated
as
such.
Here,
by
performing
multi-omics
analysis
of
siNETs,
we
unveil
four
distinct
molecular
groups
with
strong
clinical
relevance
provide
resource
study
features.
Transcriptomic,
genetic
DNA
methylation
profiles
identify
two
linked
differentiation
patterns,
another
immune
phenotype,
last
mesenchymal
properties.
This
latter
subtype
displays
worst
prognosis
resistance
treatments
in
line
infiltration
cancer-associated
fibroblasts.
These
data
insights
diversity
these
diseases,
hope
improving
management.
