BioFactors,
Journal Year:
2023,
Volume and Issue:
49(4), P. 940 - 955
Published: May 8, 2023
Abstract
Peritoneal
adhesions
are
postsurgical
fibrotic
complications
connected
to
peritoneal
inflammation.
The
exact
mechanism
of
development
is
unknown;
however,
an
important
role
attributed
activated
mesothelial
cells
(MCs)
overproducing
macromolecules
extracellular
matrix
(ECM),
including
hyaluronic
acid
(HA).
It
was
suggested
that
endogenously‐produced
HA
contributes
the
regulation
different
fibrosis‐related
pathologies.
However,
little
known
about
altered
production
in
fibrosis.
We
focused
on
consequences
increased
turnover
murine
model
adhesions.
Changes
metabolism
were
observed
early
phases
adhesion
vivo
.
To
study
mechanism,
human
MCs
MeT‐5A
and
isolated
from
peritoneum
healthy
mice
pro‐fibrotically
by
transforming
growth
factor
β
(TGFβ),
attenuated
two
modulators
carbohydrate
metabolism,
4‐methylumbelliferone
(4‐MU)
2‐deoxyglucose
(2‐DG).
attenuation
mediated
upregulation
HAS2
downregulation
HYAL2
lower
expression
pro‐fibrotic
markers,
fibronectin
α‐smooth
muscle
actin
(αSMA).
Moreover,
inclination
form
clusters
also
downregulated,
particularly
2‐DG‐treated
cells.
effects
2‐DG,
but
not
4‐MU,
changes
cellular
metabolism.
Importantly,
inhibition
AKT
phosphorylation
after
use
both
inhibitors.
In
summary,
we
identified
endogenous
as
regulator
fibrosis,
just
a
passive
player
during
this
pathological
process.
Targets,
Journal Year:
2023,
Volume and Issue:
2(1), P. 1 - 31
Published: Dec. 31, 2023
Cell-surface
glycans
are
abundant
and
complex
play
a
critical
role
in
maintaining
protein
stability,
regulating
cell
behavior,
participating
communication.
Obtaining
structural
information
on
situ
is
helpful
to
further
understand
the
of
physiological
pathological
processes
cells
regulatory
mechanism.
To
achieve
this,
we
can
use
recognition
or
labeling
strategies
convert
presence
surface
into
signals
that
be
detected.
Currently,
many
different
types
sensing
for
have
been
developed.
The
spatial
control
conversion
process
realize
restriction
glycan
detection
specific
proteins,
introduction
signal
amplification
technology
improve
sensitivity
sensing.
In
this
paper,
recent
progress
methods
reviewed,
future
development
direction
prospected.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 21, 2024
ABSTRACT
The
strain-promoted
alkyne-azide
cycloaddition
(SPAAC)
reaction
can
be
used
to
modify
the
surface
of
bacteria
for
a
variety
applications,
including
drug
delivery,
biosensing,
and
imaging.
This
is
usually
accomplished
by
first
installing
small
azide
group
within
peptidoglycan
then
delivering
exogenous
cargo
(e.g.,
protein
or
nanoparticle)
modified
with
cyclooctyne
group,
such
as
dibenzocyclooctyne
(DBCO),
in
situ
conjugation.
However,
DBCO
comparatively
bulky
hydrophobic,
increasing
propensity
some
payloads
aggregate.
In
this
study,
we
sought
invert
paradigm
exploring
two
novel
strategies
incorporating
into
Staphylococcus
aureus
compared
them
an
established
approach
using
DBCO-vancomycin.
We
demonstrate
that
DBCO-modified
molecules
belonging
all
three
classes
–
sortase
peptide
substrate
(LPETG),
D-alanine
derivatives,
vancomycin
selectively
label
S.
varying
degrees.
contrast
DBCO-vancomycin,
DBCO-D-alanine
variants
do
not
adversely
affect
growth
lead
off-target
labeling
toxicity
HEK293T
cells,
even
at
high
concentrations.
Finally,
show
that,
unlike
IgG3-Fc
labeled
groups,
groups
stable
(i.e.,
remains
water-soluble)
under
normal
storage
conditions,
retains
its
ability
bind
immune
receptor
CD64,
successfully
attached
.
believe
explored
herein
will
expand
specific,
nontoxic
SPAAC-mediated
other
gram-positive
bacteria,
opening
door
new
applications
azido-modified
cargo.
GRAPHICAL
BioFactors,
Journal Year:
2023,
Volume and Issue:
49(4), P. 940 - 955
Published: May 8, 2023
Abstract
Peritoneal
adhesions
are
postsurgical
fibrotic
complications
connected
to
peritoneal
inflammation.
The
exact
mechanism
of
development
is
unknown;
however,
an
important
role
attributed
activated
mesothelial
cells
(MCs)
overproducing
macromolecules
extracellular
matrix
(ECM),
including
hyaluronic
acid
(HA).
It
was
suggested
that
endogenously‐produced
HA
contributes
the
regulation
different
fibrosis‐related
pathologies.
However,
little
known
about
altered
production
in
fibrosis.
We
focused
on
consequences
increased
turnover
murine
model
adhesions.
Changes
metabolism
were
observed
early
phases
adhesion
vivo
.
To
study
mechanism,
human
MCs
MeT‐5A
and
isolated
from
peritoneum
healthy
mice
pro‐fibrotically
by
transforming
growth
factor
β
(TGFβ),
attenuated
two
modulators
carbohydrate
metabolism,
4‐methylumbelliferone
(4‐MU)
2‐deoxyglucose
(2‐DG).
attenuation
mediated
upregulation
HAS2
downregulation
HYAL2
lower
expression
pro‐fibrotic
markers,
fibronectin
α‐smooth
muscle
actin
(αSMA).
Moreover,
inclination
form
clusters
also
downregulated,
particularly
2‐DG‐treated
cells.
effects
2‐DG,
but
not
4‐MU,
changes
cellular
metabolism.
Importantly,
inhibition
AKT
phosphorylation
after
use
both
inhibitors.
In
summary,
we
identified
endogenous
as
regulator
fibrosis,
just
a
passive
player
during
this
pathological
process.
