bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 10, 2023
ABSTRACT
E3
ubiquitin
ligases
are
very
important
to
regulate
antiviral
immunity
during
viral
infection.
Here,
we
discovered
that
Ankyrin
repeat
and
SOCS
box-containing
protein
3
(ASB3),
an
ligase,
upregulated
in
the
presence
of
RNA
viruses,
particularly
Influenza
A
virus
(IAV).
Notably,
overexpression
ASB3
inhibits
type
I
IFN
(IFN-I)
responses
induced
by
Sendai
(SeV)
H9N2,
ablation
restores
SeV
H9N2
infection-mediated
transcription
IFN-β
its
downstream
interferon-stimulated
genes
(ISGs).
Interestingly,
animals
lacking
showed
a
decreased
susceptibility
PR8
infections.
Mechanistically,
interacts
with
MAVS
directly
mediates
K48-linked
polyubiquitination
degradation
at
K297,
thereby
inhibiting
phosphorylation
levels
TBK1
IRF3,
downregulating
signaling.
These
findings
establish
as
critical
negative
regulator
controlling
activation
signaling
describe
novel
function
has
not
been
previously
reported.
IMPORTANCE
IAV
is
significant
zoonotic
pathogen
causes
infections
respiratory
system.
Hosts
have
evolved
multiple
strategies
defend
against
However,
all
host
proteins
play
active
defense
role.
In
this
study,
found
ligase
regulates
manipulating
stability.
Briefly,
degrades
MAVS,
promoting
replication.
contrast,
deletion
expression,
upregulating
IFN-I
responses.
Additional
research
revealed
resulting
being
degraded
via
ubiquitin-proteasome
pathway.
reveal,
for
first
time,
mechanism
which
negatively
provides
potential
target
anti-IAV
drug
development.
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(29)
Published: July 10, 2024
Defects
in
planar
cell
polarity
(PCP)
have
been
implicated
diverse
human
pathologies.
Vangl2
is
one
of
the
core
PCP
components
crucial
for
signaling.
Dysregulation
has
associated
with
severe
neural
tube
defects
and
cancers.
However,
how
protein
regulated
at
posttranslational
level
not
well
understood.
Using
chemical
reporters
fatty
acylation
biochemical
validation,
here
we
present
that
subcellular
localization
by
a
reversible
S-stearoylation
cycle.
The
dynamic
process
mainly
acyltransferase
ZDHHC9
deacylase
acyl-protein
thioesterase
1
(APT1).
stearoylation-deficient
mutant
shows
decreased
plasma
membrane
localization,
resulting
disruption
establishment
during
migration.
Genetically
or
pharmacologically
inhibiting
phenocopies
effects
stearoylation
loss
Vangl2.
In
addition,
enhances
activation
oncogenic
Yes-associated
(YAP),
serine-threonine
kinase
AKT,
extracellular
signal-regulated
(ERK)
signaling
promotes
breast
cancer
growth
HRas
G12V
(HRas
V12
)-induced
transformation.
Our
results
reveal
regulation
mechanism
Vangl2,
provide
mechanistic
insight
into
acid
metabolism
regulate
tumorigenesis
lipidation.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 16, 2024
Abstract
Background
The
respiratory
tract
houses
a
specialized
microbial
ecosystem,
and
despite
the
close
anatomical
physiological
ties
between
oral,
upper
respiratory,
lower
tracts,
there
is
substantial
discrepancy
in
quantity,
spanning
multiple
orders
of
magnitude.
potential
for
commensal
bacteria
to
prevent
infection
lies
their
ability
regulate
innate
adaptive
host
immune
responses.
Influenza
virus
predominantly
targets
replicates
within
epithelial
cells
both
tracts.
Given
this,
we
hypothesize
that
nasal-lung-microbe
cross-talk
plays
crucial
role
influencing
influenza
susceptibility.
In
this
study,
investigated
viral
presence,
gene
expression
profiles
host,
nasal
lung
microbiota
beagle
dog
model
with
antibiotic-induced
dysbiosis
during
infection.
Results
using
16S
rRNA
sequencing,
combined
comparative
anatomy,
transcriptomics
histological
examination,
influenza-infected
beagles
dysbiosis.
Our
data
showed
microbiome
exacerbates
influenza-induced
disease
barrier
disruption,
impairs
antiviral
responses
cavity
lung.
Moreover,
dysregulation
worsens
disturbance
microbiota.
Further,
identified
one
strain
Lactobacillus
plantarum
significant
effect,
which
exerted
by
activating
IFN
pathway
modulating
impaired
autophagy
flux
induced
virus.
collectively
indicate
connection
microbiomes
different
ecological
niches
regions.
This
significantly
influences
subsequent
host-microbe
cross-talk,
was
associated
an
increased
susceptibility
influenza.
Conclusions
investigation
reveals
not
only
increases
but
also
contributes
exacerbation
dysregulation.
intricate
relationship
extends
composition,
demonstrating
correlations
critical
factors
such
as
responses,
inflammation
thresholds,
mucosal
integrity.
Together,
these
findings
underscore
impact
on
overall
outcome
infections.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 10, 2023
ABSTRACT
E3
ubiquitin
ligases
are
very
important
to
regulate
antiviral
immunity
during
viral
infection.
Here,
we
discovered
that
Ankyrin
repeat
and
SOCS
box-containing
protein
3
(ASB3),
an
ligase,
upregulated
in
the
presence
of
RNA
viruses,
particularly
Influenza
A
virus
(IAV).
Notably,
overexpression
ASB3
inhibits
type
I
IFN
(IFN-I)
responses
induced
by
Sendai
(SeV)
H9N2,
ablation
restores
SeV
H9N2
infection-mediated
transcription
IFN-β
its
downstream
interferon-stimulated
genes
(ISGs).
Interestingly,
animals
lacking
showed
a
decreased
susceptibility
PR8
infections.
Mechanistically,
interacts
with
MAVS
directly
mediates
K48-linked
polyubiquitination
degradation
at
K297,
thereby
inhibiting
phosphorylation
levels
TBK1
IRF3,
downregulating
signaling.
These
findings
establish
as
critical
negative
regulator
controlling
activation
signaling
describe
novel
function
has
not
been
previously
reported.
IMPORTANCE
IAV
is
significant
zoonotic
pathogen
causes
infections
respiratory
system.
Hosts
have
evolved
multiple
strategies
defend
against
However,
all
host
proteins
play
active
defense
role.
In
this
study,
found
ligase
regulates
manipulating
stability.
Briefly,
degrades
MAVS,
promoting
replication.
contrast,
deletion
expression,
upregulating
IFN-I
responses.
Additional
research
revealed
resulting
being
degraded
via
ubiquitin-proteasome
pathway.
reveal,
for
first
time,
mechanism
which
negatively
provides
potential
target
anti-IAV
drug
development.