Multi‐omics analyses reveal the signatures of metabolite transfers across trophic levels in a high‐CO₂ ocean

Limnology and Oceanography, Journal Year: 2024, Volume and Issue: 69(8), P. 1667 - 1682

Published: June 24, 2024

Abstract Although the diverse impacts of elevated dissolved CO 2 and warming on organisms within various trophic levels in marine food webs are well documented, we have yet to explore biological links across different organization from primary producers secondary an evolutionary time scale a high‐CO ocean. Here, cultured model diatom Phaeodactylum tricornutum (primary producer) predicted future and/or conditions for ~ 1250 d with experimental evolution approach then fed them clam Coelomactra antiquata (secondary producer). We present in‐depth multi‐omics analysis along methylome producer)–transcriptome producer)–metabolome continuum. Our results showed that downregulated terpenoid backbone biosynthesis transcriptome lead decreased pyruvate upregulation some pathways (such as phenylalanine metabolism) metabolome producer long‐term conditions. These changes metabolomic profile were transferred producer, resulting abundance metabolites, such decreases pyruvate, pyruvaldhyde (also known methylglyoxal), increases 2‐hydroxylamino‐4,6‐dinitrotoluene. study provides new insight into molecular mechanisms underlying transfer ocean may provide more accurate projections ecosystem services functions over next century.

Language: Английский

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Recommendations for advancing mixoplankton research through empirical-model integration

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: June 5, 2024

Protist plankton can be divided into three main groups: phytoplankton, zooplankton, and mixoplankton. In situ methods for studying phytoplankton zooplankton are relatively straightforward since they generally target chlorophyll/photosynthesis or grazing activity, while the integration of both processes within a single cell makes mixoplankton inherently challenging to study. As result, we understand less about physiology their role in food webs, biogeochemical cycling, ecosystems compared zooplankton. this paper, posit that by merging conventional techniques, such as microscopy physiological data, with innovative like single-cell sorting omics datasets, conjunction diverse array modeling approaches ranging from comprehensive Earth system models, propel research forefront aquatic ecology. We present eight crucial questions pertaining mixotrophy, briefly outline combination existing models used address each question. Our intent is encourage more interdisciplinary on mixoplankton, thereby expanding scope data acquisition knowledge accumulation understudied yet critical component ecosystems.

Language: Английский

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Climate Change and terrigenous inputs decrease the efficiency of the future Arctic Ocean’s biological carbon pump

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 21, 2024

Abstract The Arctic experiences climate changes that are among the fastest in world and affect all Earth system components. Despite expected increase terrigenous inputs to Ocean, their impacts on biogeochemical cycles currently largely neglected IPCC-like models. We used a state-of-the-art high-resolution ocean biogeochemistry model, includes carbon nutrient from rivers coastal erosion, produce twenty-first-century pan-Arctic projections. Surprisingly, even with an anticipated rise primary production across wide range of emission scenarios, our findings indicate change leads counterintuitive 40% reduction efficiency Arctic's biological pump by 2100, which contribute 10%. Terrigenous will also drive intense CO 2 outgassing, reducing Ocean's sink at least 10% (33 TgC yr -1 ). These unexpected positive feedbacks, mostly due accelerated remineralization rates, lower Ocean’s capacity for sequestering carbon.

Language: Английский

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Reframing trait trade-offs in marine microbes

Communications Earth & Environment, Journal Year: 2024, Volume and Issue: 5(1)

Published: April 24, 2024

Abstract The oceans sequester a vast amount of carbon thus playing central role in the global cycle. Assessing how cycling will be impacted by climate change requires an improved understanding microbial dynamics, which are responsible for most transformations oceans. Current numerical models used predicting future states represent simplified phenotypes and may not produce robust predictions communities. We propose reframing approaches studying trait to allow selection on multi-trait phenotypes. Integrating statistical trait-based incorporation evolution into cycle predictions.

Language: Английский

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Nutritional thermal ecology: investigating the combined influence of temperature and nutrient availability on plant‐ectotherm trophic interactions

Oikos, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Many primary consumers in freshwater, marine and terrestrial systems are ectotherms (e.g. zooplankton insects), whose metabolisms, therefore nutritional demands, modulated by temperature. Further, nutrient availability largely influences the quality of resources consumed these organisms, hence affects whether demands fulfilled. From considerations, a crucial question arises: how do temperature together modulate trophodynamics at basis food webs? Addressing this for insects is essential since most abundant metazoans on Earth, they link production to higher trophic levels. Here, we synthesize existing literature offer avenues guide future scientific endeavours. We highlight that vast majority studies combined influence published date focus least one following research topics: 1) metabolic requirements ectotherms; 2) feeding behaviour; 3) eco‐evolutionary processes; 4) trophodynamics. pose further advances field may provide robust understanding modulations consumer resource define consumer–producer interactions across marine, freshwater ecosystems. This effort would enable combine fields Ecological stoichiometry Metabolic theory ecology, create an integrated approach, which propose call Nutritional thermal ecology.

Language: Английский

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Warming and polymetallic stress induce proteomic and physiological shifts in the neurotoxic Alexandrium pacificum as possible response to global changes

Marine Pollution Bulletin, Journal Year: 2024, Volume and Issue: 209, P. 117221 - 117221

Published: Nov. 9, 2024

Language: Английский

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Differential impacts of temperature increase on prokaryotes across temperature regimes in subtropical coastal waters: insights from field experiments

Limnology and Oceanography, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Abstract Prokaryotic communities play a dominant role in driving biogeochemical cycling marine ecosystems. How short‐term temperature increase impacts prokaryotes subtropical coastal waters is still largely unknown. Here, 14 field experiments were conducted to investigate the response of increases 3°C and 6°C, encompassing range ambient temperatures from 17°C 31°C. We found that responses prokaryotic growth, grazing pressure, community, transcriptomes increased affected by temperatures. Increased enhanced growth rate pressure heterotrophic when below 26–28°C. The had greater negative effects on compared rate; therefore, abundance generally after across all regimes. Metatranscriptomics analysis showed at an 30°C, genes involved adenosine triphosphate synthase significantly downregulated temperature. This could be major factor contributing decreased rate. In comparison, autotrophic ( Synechococcus ) exhibited better performance elevated temperatures, thriving up 35°C, beyond which their experienced dramatic decline. When exposing extremely high photosynthesis decreased. These findings highlight differential ecological communities, varying different taxa waters.

Language: Английский

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Direct quantification of unicellular algae sinking velocities reveals cell size, light, and nutrient-dependence

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 23, 2023

ABSTRACT Eukaryotic phytoplankton, also known as algae, form the basis of marine food webs and drive carbon sequestration when their biomass sinks to ocean floor. Algae must regulate vertical movement, determined by motility gravitational sinking, balance access light at surface nutrients in deeper layers. However, regulation sinking velocities remains largely unknown, especially motile species. Here, we directly quantify single-cell masses volumes calculate according Stokes’ law diverse clades unicellular microalgae. Our results reveal cell size, light, nutrient-dependency velocities. We identify dinoflagellate green algal species that increase velocity response starvation. Mechanistically, this increased is achieved photosynthesis-driven accumulation carbohydrates, which increases mass density. Moreover, correlate inversely with proliferation rates, mechanism regulating integrates signals from multiple nutrients. findings suggest composition environmental conditions contributes movement cells oceans. More broadly, our approach for measurements expands study supports modeling pump nutrient cycles.

Language: Английский

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