Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 3, 2025
The
E3
ubiquitin
ligase
RNF112
is
significantly
downregulated
in
bladder
cancer
(BLCA)
and
correlated
with
disease
progression.
In
vitro
vivo
studies
indicated
that
suppresses
BLCA
cell
proliferation,
migration,
lipid
synthesis.
Mechanistically,
directly
interacts
the
MB
II
domain
of
MYC
through
its
N-terminal
zinc
finger
motif,
catalytic
site
C97
facilitates
K48-linked
polyubiquitination
K389
residue
on
c-Myc
protein,
accelerating
degradation.
Additionally,
this
research
validated
interaction
promoter
ATP
citrate
lyase
(ACLY),
a
central
enzyme
metabolism,
promoting
transcriptional
activity.
restoration
or
ACLY
expression
attenuated
inhibitory
effects
growth,
conclusion,
study
confirmed
suppressed
synthesis
cells
by
facilitating
ubiquitin-mediated
degradation
c-Myc.
ABSTRACT
This
study
investigates
the
synergistic
inhibitory
effects
of
combining
stimulator
interferon
genes
(STING)
agonist
cyclic
diadenylate
monophosphate
(c‐di‐AMP)
and
ginsenoside
RG3
on
cisplatin
(DDP)‐resistant
gastric
cancer
(GC)
cells.
The
objective
is
to
identify
novel
therapeutic
targets
offers
insights
for
clinical
management
DDP
resistance.
Various
techniques
were
employed,
including
western
blot,
MTT
assay,
colony
formation
scratch
transwell
tubule
flow
cytometry,
Hoechst
33342
fluorescence
staining,
in
vivo
experiments,
investigate
potential
mechanisms
combined
application
STING
reversing
resistance
cancer.
combination
markedly
suppressed
key
malignant
behaviors,
proliferation,
migration,
invasion,
angiogenesis
SGC‐7901/DDP
Additionally,
this
treatment
inhibited
epithelial‐mesenchymal
transition
(EMT)
process
stem
cell‐like
characteristics
cells,
while
downregulating
expression
resistance‐related
proteins.
effectively
suppresses
growth
proliferation
Ginsenoside
RG3,
well‐documented
its
multifaceted
properties,
antioxidant,
anti‐aging,
anti‐cancer
effects,
widely
used
managing
chemotherapy‐related
side
effects.
Furthermore,
enhances
anti‐tumor
immunity
by
regulating
signal
transduction.
comprehensively
evaluated
efficacy
through
vitro
demonstrating
significant
inhibition
progression
reversal
drug
These
findings
offer
a
robust
theoretical
foundation
applications
highlight
new
future
research.
Cell Reports,
Journal Year:
2025,
Volume and Issue:
44(2), P. 115284 - 115284
Published: Feb. 1, 2025
ATP-citrate
lyase
(ACLY)
generates
cytosolic
acetyl-coenzyme
A
(acetyl-CoA)
for
lipid
synthesis
and
is
a
promising
therapeutic
target
in
diseases
with
altered
metabolism.
Here,
we
developed
inducible
whole-body
Acly-knockout
mice
to
determine
the
requirement
ACLY
normal
tissue
functions,
uncovering
its
crucial
role
skin
homeostasis.
ACLY-deficient
upregulates
acetyl-CoA
synthetase
ACSS2;
deletion
of
both
Acly
Acss2
from
exacerbates
abnormalities,
differential
effects
on
two
major
lipid-producing
compartments.
While
epidermis
depleted
barrier
lipids,
sebaceous
glands
increase
production
sebum,
supplied
at
least
part
by
circulating
fatty
acids
coinciding
adipose
lipolysis
fat
depletion.
Dietary
supplementation
further
boosts
sebum
partially
rescues
lipoatrophy
aberrant
phenotypes.
The
data
establish
critical
maintaining
integrity
highlight
as
key
organ
systemic
regulation.
Vaccines,
Journal Year:
2025,
Volume and Issue:
13(2), P. 182 - 182
Published: Feb. 13, 2025
Histone
deacetylase
3
(HDAC3)
has
emerged
as
a
critical
epigenetic
regulator
in
tumor
progression
and
immune
modulation,
positioning
it
promising
target
for
enhancing
cancer
immunotherapy.
This
work
comprehensively
explores
HDAC3's
multifaceted
roles,
focusing
on
its
regulation
of
key
immune-modulatory
pathways
such
cGAS-STING,
ferroptosis,
the
Nrf2/HO-1
axis.
These
are
central
to
evasion,
antigen
presentation,
cell
activation.
Additionally,
distinct
effects
HDAC3
various
types-including
role
T
activation,
restoring
NK
cytotoxicity,
promoting
dendritic
maturation,
modulating
macrophage
polarization-are
thoroughly
examined.
findings
underscore
capacity
reshape
microenvironment,
converting
immunologically
"cold
tumors"
into
"hot
thereby
increasing
their
responsiveness
The
therapeutic
potential
inhibitors
is
highlighted,
both
standalone
agents
combination
with
checkpoint
inhibitors,
overcome
resistance
improve
treatment
efficacy.
Innovative
strategies,
development
selective
advanced
nano-delivery
systems,
integration
photodynamic
or
photothermal
therapies,
proposed
enhance
precision
minimize
toxicity.
By
addressing
challenges
toxicity,
patient
heterogeneity,
mechanisms,
this
study
provides
forward-looking
perspective
clinical
application
inhibitors.
It
highlights
significant
personalized
immunotherapy,
paving
way
more
effective
treatments
improved
outcomes
patients.
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(3), P. 329 - 329
Published: Feb. 24, 2025
The
recognition
of
cytosolic
nucleic
acids
is
a
critical
step
in
the
host
immune
response
against
danger
signals,
such
as
molecular
patterns
from
pathogens
or
tissue
damage.
Nonetheless,
over-reactivity
to
self-nucleic
leads
sustained
production
type
I
interferon
(IFN),
mediated
either
by
cGAS
RLR,
contributing
pathogenesis
certain
autoimmune
diseases,
Aicardi–Goutières
syndrome
(AGS).
Therefore,
inhibiting
excessive
IFN
represents
potential
therapeutic
strategy
for
conditions.
In
this
study,
we
discovered
that
petroselinic
acid
(PA),
natural
compound
isolated
Apiaceae
family
plants,
effectively
suppresses
induced
acids.
Mechanistic
investigations
revealed
PA
inhibits
phosphorylation
TBK1
and
IRF3,
which
are
key
nodal
proteins
within
pathway.
Notably,
docking
suggests
binding
between
sensors,
RIG-I.
Moreover,
found
attenuates
expression
their
downstream
interferon-stimulated
genes
(ISGs)
models
AGS
disease
characterized
accumulation.
Thus,
our
research
identifies
offers
promising
treating
diseases
resulting
aberrant
hyperactivation
interferon.
