Stem Cell Research & Therapy,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Nov. 15, 2024
Exosomes
(Exos)
from
adipose-derived
stem
cells
(ADSCs)
have
a
high
inclusion
content
and
low
immunogenicity,
which
helps
to
control
inflammation
accelerate
the
healing
of
wounds.
Unfortunately,
yield
exosomes
is
poor,
raises
expense
lengthens
treatment
period
in
addition
impairing
exosomes'
therapeutic
impact.
Thus,
one
key
problems
that
needs
be
resolved
current
exosome
study
increasing
yield.
Tetraspanin-6
(TSPAN6)
overexpression
knockdown
models
ADSCs
were
constructed
determine
number
secreted
by
each
group
as
well
multivesicular
bodies
(MVBs)
intraluminal
vesicles
(ILVs)
within
cells.
Subsequently,
binding
region
interaction
between
TSPAN6
syntenin-1
was
identified
using
yeast
two-hybrid
assay,
itself
immunoprecipitation.
Finally,
cellular
animal
studies
conducted
investigate
role
class
exosomes.
When
compared
group,
intracellular
MVBs
ILVs
significantly
larger,
ADSCsTSPAN6+-Exos
more
than
three
times
higher.
However,
TSPAN6's
ability
stimulate
secretion
reduced
result
knockdown.
Additional
assay
demonstrated
critical
structures
for
their
N-terminal,
Postsynaptic
density
protein
95/Discs
large
protein/Zonula
occludens
1
(PDZ1),
PDZ2
domains
syntenin-1,
C-terminal
TSPAN6.
In
trials,
wound
rate
best
while
experiments
better
enhanced
proliferation
migration
human
skin
fibroblasts
(HSFs)
umbilical
vein
endothelial
(HUVECs).
stimulates
formation,
creation
ADSCs.
Syntenin-1
essential
stimulation
ADSCs-Exos
secretion.
Furthermore,
has
greater
support
healing,
angiogenesis,
variety
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Aug. 5, 2024
Wound
healing
is
a
complicated
obstacle,
especially
for
chronic
wounds.
Mesenchymal
stem
cell-derived
exosomes
may
be
promising
cell-free
approach
treating
skin
wound
healing.
Exosomes
can
accelerate
by
attenuating
inflammation,
promoting
angiogenesis,
cell
proliferation,
extracellular
matrix
production
and
remodeling.
However,
many
issues,
such
as
off-target
effects
high
degradation
of
in
sites
need
to
addressed
before
applying
into
clinical
therapy.
Therefore,
the
bioengineering
technology
has
been
introduced
modify
with
greater
stability
specific
therapeutic
property.
To
prolong
function
time
local
concentration
bed,
use
biomaterials
load
emerges
strategy.
In
this
review,
we
summarize
biogenesis
characteristics
exosomes,
role
healing,
applications
modified-exosomes
The
challenges
prospects
are
also
discussed.
Stem Cell Research & Therapy,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Nov. 15, 2024
Exosomes
(Exos)
from
adipose-derived
stem
cells
(ADSCs)
have
a
high
inclusion
content
and
low
immunogenicity,
which
helps
to
control
inflammation
accelerate
the
healing
of
wounds.
Unfortunately,
yield
exosomes
is
poor,
raises
expense
lengthens
treatment
period
in
addition
impairing
exosomes'
therapeutic
impact.
Thus,
one
key
problems
that
needs
be
resolved
current
exosome
study
increasing
yield.
Tetraspanin-6
(TSPAN6)
overexpression
knockdown
models
ADSCs
were
constructed
determine
number
secreted
by
each
group
as
well
multivesicular
bodies
(MVBs)
intraluminal
vesicles
(ILVs)
within
cells.
Subsequently,
binding
region
interaction
between
TSPAN6
syntenin-1
was
identified
using
yeast
two-hybrid
assay,
itself
immunoprecipitation.
Finally,
cellular
animal
studies
conducted
investigate
role
class
exosomes.
When
compared
group,
intracellular
MVBs
ILVs
significantly
larger,
ADSCsTSPAN6+-Exos
more
than
three
times
higher.
However,
TSPAN6's
ability
stimulate
secretion
reduced
result
knockdown.
Additional
assay
demonstrated
critical
structures
for
their
N-terminal,
Postsynaptic
density
protein
95/Discs
large
protein/Zonula
occludens
1
(PDZ1),
PDZ2
domains
syntenin-1,
C-terminal
TSPAN6.
In
trials,
wound
rate
best
while
experiments
better
enhanced
proliferation
migration
human
skin
fibroblasts
(HSFs)
umbilical
vein
endothelial
(HUVECs).
stimulates
formation,
creation
ADSCs.
Syntenin-1
essential
stimulation
ADSCs-Exos
secretion.
Furthermore,
has
greater
support
healing,
angiogenesis,
variety
