Tracing of the developmental origin of the caudal fin muscle in zebrafish DOI Creative Commons
Kinya G. Ota, Gembu Abe

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Teleost species possess complex caudal musculoskeletal systems. While mid-trunk muscles exhibit simple segmental patterns, several skeletal display intricate orientations in their muscle fibers. Due to this distinctive morphology, both early and recent researchers have studied the structure development of system. However, developmental origin cell populations within system remains largely unknown. In study, we performed lineage tracing primordia zebrafish using a transgenic line expressing EGFP somite derivatives following tamoxifen induction. This approach allowed us observe specific that contribute tissue formation at larval stage. By monitoring growth these labeled cells from stage, identified origins fibers fin unique teleosts, such as adductor caudalis flexor caudalis. Our findings provide descriptions aid understanding how fish-specialized structures were formed through modification processes during evolution.

Language: Английский

Fin elaboration via anterior-posterior constraint by hhip on Hedgehog signaling in teleosts DOI Creative Commons
Yoshitaka Tanaka,

S. Okayama,

Kayoko Urakawa

et al.

Development, Journal Year: 2024, Volume and Issue: 151(22)

Published: Oct. 17, 2024

ABSTRACT Pectoral fins, the anterior paired fins in fish, have enhanced maneuvering abilities due to morphological changes. Teleosts fewer radial bones their pectoral than basal species, resulting more-elaborate fins. The mechanism behind this constraint change teleosts is unclear. Here, we found that mutations hhip, which encodes an antagonist of Hedgehog signaling, led increase a localized region. Expression Shh genes, encoding ligands coincided with notable hhip expression specifically during early development. We suggest negative feedback effect signaling by regulates fin zebrafish. Additionally, re-analysis hhip-related gene data zebrafish and species revealed development characteristic not observed species. Region-specific Hox13 genes indicated median region, analogous region abundant radials expanded hhip−/− These underscore potential evolution through constrained diversity.

Language: Английский

Citations

1
Genome-Wide Identification of DNA Methylation Marks Associated with Resistance to Streptococcus Agalactiae in the Gift Strain of Nile Tilapia (Oreochromis Niloticus) DOI

Tao Fei Qiao,

Ji Ping Jiao,

Dan Dan Huang

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Adult caudal fin shape is imprinted in the embryonic fin fold DOI
E. Marc SURETTE, Joan Donahue, Stephanie A. Robinson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

Summary Appendage shape is formed during development (and re-formed regeneration) according to spatial and temporal cues that orchestrate local cellular morphogenesis. The caudal fin the primary appendage used for propulsion in most fish species, exhibits a range of distinct morphologies adapted different swimming strategies, however molecular mechanisms responsible generating these diverse shapes remain mostly unknown. In zebrafish, fins display forked shape, with longer supportive bony rays at periphery shortest center. Here, we show premature, transient pulse sonic hedgehog (shha) overexpression late embryonic results excess proliferation growth central rays, causing adult grow into triangular, truncate shape. Both global regional ectopic shha are sufficient alter may be rescued by subsequent treatment an antagonist canonical Shh pathway. induced decreased ray number fail form hypural diastema normally separates dorsal ventral lobes. While regenerate their original morphology, truncate, suggesting positional memory can permanently altered embryogenesis. Ray finned have evolved wide spectrum morphologies, ranging from forked, current work offers insights developmental underlie this diversity.

Language: Английский

Citations

0
Epigenomics of embryogenesis in turbot (Scophthalmus maximus) DOI Open Access
Óscar Aramburu, Belén G. Pardo, Ada Jiménez-González

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

ABSTRACT Embryogenesis is the crucial first step of ontogeny, where an organism with a complex body plan arises from single undifferentiated totipotent cell. This process orchestrated by dynamic changes in transcriptional regulation, influenced chromatin accessibility and nucleotide histone modifications constituting epigenetic signals enabling access to transcription factors. The epigenomic regulation embryogenesis has been studied model fishes, but little attention paid farmed fish - traits importance aquaculture rely on early developmental processes. study, framed within AQUA-FAANG consortium, reports comprehensive regulatory atlas for turbot ( Scophthalmus maximus ), flatfish representing order Pleuronectiformes. 14,560 genes were expressed embryonic transcriptome > 90% showing differential expression across consecutive stages. By integrating multi-histone ChIP-Seq marks ATAC-Seq, we built genome-wide state model, defining promoter enhancer activity Transcription factor binding motif (TFBM) analysis differentially active promoters enhancers revealed dynamism regulated gene functions, more than half TFBM enriched transition. Significant shifts occurred stages, most notably during transition shield segmentation, suggesting profound reorganization underpins somitogenesis organ development. Most stages did not involve regions genes, trend preceding activity. Comparative analyses zebrafish global transcriptomic correlation copy orthologs at matched species. While conserved dynamics many orthologous Hox notable cross-species differences identified before zygotic genome activation leading up hatching. multi-omics investigation provides novel non-coding elements controlling development, key applications biology enhancing sustainable aquaculture.

Language: Английский

Citations

0
Tracing of the developmental origin of the caudal fin muscle in zebrafish DOI Creative Commons
Kinya G. Ota, Gembu Abe

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Teleost species possess complex caudal musculoskeletal systems. While mid-trunk muscles exhibit simple segmental patterns, several skeletal display intricate orientations in their muscle fibers. Due to this distinctive morphology, both early and recent researchers have studied the structure development of system. However, developmental origin cell populations within system remains largely unknown. In study, we performed lineage tracing primordia zebrafish using a transgenic line expressing EGFP somite derivatives following tamoxifen induction. This approach allowed us observe specific that contribute tissue formation at larval stage. By monitoring growth these labeled cells from stage, identified origins fibers fin unique teleosts, such as adductor caudalis flexor caudalis. Our findings provide descriptions aid understanding how fish-specialized structures were formed through modification processes during evolution.

Language: Английский

Citations

0