Multiomics analyses decipher intricate changes in the cellular and metabolic landscape of steatotic livers upon dietary restriction and sleeve gastrectomy DOI Creative Commons
Shuai Chen, Qinghe Zeng, Xiurong Cai

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(11), P. 4438 - 4457

Published: Jan. 1, 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a chronic, progressive that encompasses spectrum of steatosis, steatohepatitis (or MASH), and fibrosis. Evidence suggests dietary restriction (DR) sleeve gastrectomy (SG) can lead to remission hepatic steatosis inflammation through weight loss, but it unclear whether these procedures induce distinct metabolic or immunological changes in MASLD livers. This study aims elucidate the intricate following DR, SG sham surgery rats fed high-fat diet as model obesity-related MASLD, comparison clinical cohort patients undergoing SG. Single-cell single-nuclei transcriptome analysis, spatial metabolomics, immunohistochemistry revealed landscape, while circulating biomarkers were measured serum samples. Artificial intelligence (AI)-assisted image analysis characterized distribution hepatocytes, myeloid cells lymphocytes. In experimental rats, improved body mass index, injury triglyceride levels. Both DR attenuated fibrosis rats. Metabolism-related genes (

Language: Английский

Dual-release hydrocortisone treatment improves serum and peripheral blood mononuclear cell inflammatory and immune profiles in patients with autoimmune primary adrenal insufficiency DOI Creative Commons
Laura Tomasello, Antonina Coppola, Giuseppe Pizzolanti

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 23, 2025

Objective The primary outcome was the evaluation of T-cell phenotype in autoimmune adrenal insufficiency (PAI). Secondary outcomes included CD4 + CD25 Foxp3 Treg population and gene expression levels IL-6, IL-17A, cyclooxygenase (COX)-2, heat shock proteins (HSP)-70, indoleamine-2,3-dioxygenase (IDO), programmed death-ligand 1 (PD-L1), inducible nitric oxide synthase (iNOS), thioredoxin (TXN)-1. Methods We prospectively 15 patients with PAI on conventional glucocorticoid (GC) replacement therapy, switched to dual-release hydrocortisone (DR-HC), 20 healthy controls. Serum inflammatory parameters peripheral blood mononuclear cells (PBMCs) were evaluated at baseline after 12 months treatment. Result At baseline, significantly higher CD8 (both p < 0.001) percentages, a lower /CD8 ratio ( 0.05), /CD25 T observed compared After DR-HC treatment, we found IL-6 = 0.019), IL-17A 0.046), COX-2 0.001), HSP-70 0.006), TXN-1 0.008) PD-L1 IDO mRNA values baseline. significant increase 0.012), 0.003), decrease 0.0014), 0.005), 0.0008), as well conversion 0.033), GCs. Conclusions switch from GCs treatment altered lymphocyte Treg-independent manner, inducing improvements immune profile PAI.

Language: Английский

Citations

0
Experimental verification of Myriocin molecular bioactivity in revoking rheumatoid arthritis: New emphasis on S100A1 repression and PPAR-γ activation via non-canonical pathways in the flare-up of cytokines DOI

Ghada S. El‐Tanbouly

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 158, P. 114811 - 114811

Published: May 11, 2025

Language: Английский

Citations

0
The role of hypoxic microenvironment in autoimmune diseases DOI Creative Commons
Xun Gong,

S. Yang,

Zhenyu Wang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 7, 2024

The hypoxic microenvironment, characterized by significantly reduced oxygen levels within tissues, has emerged as a critical factor in the pathogenesis and progression of various autoimmune diseases (AIDs). Central to this process is hypoxia-inducible factor-1 (HIF-1), which orchestrates wide array cellular responses under low conditions. This review delves into multifaceted roles microenvironment modulating immune cell function, particularly highlighting its impact on activation, metabolic reprogramming, angiogenesis. Specific focus given mechanisms hypoxia contributes development exacerbation such rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), multiple sclerosis (MS), dermatomyositis (DM). In these conditions, not only disrupts tolerance but also enhances inflammatory promotes tissue damage. discusses emerging therapeutic strategies aimed at targeting pathways, including application HIF-1α inhibitors, mTOR other modulators response. By providing comprehensive overview interplay between dysfunction AIDs, offers new perspectives underlying highlights potential avenues for intervention.

Language: Английский

Citations

3
Multiomics analyses decipher intricate changes in the cellular and metabolic landscape of steatotic livers upon dietary restriction and sleeve gastrectomy DOI Open Access
Shuai Chen, Qinghe Zeng, Xiurong Cai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 17, 2024

Abstract Metabolic dysfunction-associated steatotic liver disease (MASLD) is a chronic, progressive that encompasses spectrum of steatosis, steatohepatitis (or MASH), and fibrosis. Evidence suggests dietary restriction (DR) sleeve gastrectomy (SG) can lead to remission hepatic steatosis inflammation through weight loss, but it unclear whether these procedures induce distinct metabolic or immunological changes in MASLD livers. This study aims elucidate the intricate following DR, SG sham surgery rats fed high-fat diet as model obesity-related MASLD, comparison clinical cohort patients undergoing SG. Single-cell single-nuclei transcriptome analysis, spatial metabolomics, immunohistochemistry revealed landscape, while circulating biomarkers were measured serum samples. Artificial intelligence (AI)-assisted image analysis characterized distribution hepatocytes, myeloid cells lymphocytes. In experimental rats, improved BMI, injury triglyceride levels. Both DR attenuated fibrosis rats. Metabolism-related genes ( Ppara , Cyp2e1 Cyp7a1 ) upregulated hepatocytes upon SG, broadly lipid metabolism on cholangiocytes, monocytes, macrophages, neutrophils. Furthermore, promoted cell accumulation not only ameliorating activating repair processes. Regions with potent infiltration marked enhanced capacities Additionally, disruption periportal hepatocyte functions was observed DR. conclusion, this indicates dynamic cellular crosstalk livers Notably, PPARα- gut-liver axis-related processes, metabolically-active indicate intervention-related mechanisms supporting for treatment MASLD.

Language: Английский

Citations

0
Multiomics analyses decipher intricate changes in the cellular and metabolic landscape of steatotic livers upon dietary restriction and sleeve gastrectomy DOI Creative Commons
Shuai Chen, Qinghe Zeng, Xiurong Cai

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(11), P. 4438 - 4457

Published: Jan. 1, 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a chronic, progressive that encompasses spectrum of steatosis, steatohepatitis (or MASH), and fibrosis. Evidence suggests dietary restriction (DR) sleeve gastrectomy (SG) can lead to remission hepatic steatosis inflammation through weight loss, but it unclear whether these procedures induce distinct metabolic or immunological changes in MASLD livers. This study aims elucidate the intricate following DR, SG sham surgery rats fed high-fat diet as model obesity-related MASLD, comparison clinical cohort patients undergoing SG. Single-cell single-nuclei transcriptome analysis, spatial metabolomics, immunohistochemistry revealed landscape, while circulating biomarkers were measured serum samples. Artificial intelligence (AI)-assisted image analysis characterized distribution hepatocytes, myeloid cells lymphocytes. In experimental rats, improved body mass index, injury triglyceride levels. Both DR attenuated fibrosis rats. Metabolism-related genes (

Language: Английский

Citations

0