The presence of nanoparticles in aqueous droplets containing plant-derived biopolymers plays a role in heterogeneous ice nucleation DOI
Paul Bieber,

Ghinwa H. Darwish,

W. Russ Algar

et al.

The Journal of Chemical Physics, Journal Year: 2024, Volume and Issue: 161(9)

Published: Sept. 4, 2024

Organic matter can initiate heterogeneous ice nucleation in supercooled water droplets, thereby influencing atmospheric cloud glaciation. Predicting the ability of organic matter-containing droplets is challenging due to unknown mechanism for templating ice. Here, we observed presence nanoparticles aqueous samples known ice-nucleating biopolymers cellulose and lignin, as well newly identified xylan laminarin. Using our drop Freezing Ice Nuclei Counter (FINC), measured median temperature (T50) laminarin 2 μl be −14.2 −20.0 °C, respectively. Next, characterized these using nanoparticle tracking analysis, detected quantified with mean diameters between 132 267 nm. Xylan contained largest froze at higher temperatures. also dictated freezing a 1:1:1:1 mixture cellulose, laminarin, xylan. Filtration experiments down 300 kDa sample indicated that triggered freezing. Overall, only above 150 nm −20 °C. Furthermore, determined ice-active site densities normalized particle concentrations, surface area, mass show samples’ are similar sea spray aerosols nanometer-sized dust. The identification characterization substances expands growing list capable impacting formation thus climate.

Language: Английский

Aggregation of ice-nucleating macromolecules from Betula pendula pollen determines ice nucleation efficiency DOI Creative Commons
Florian Reyzek,

Nadine Bothen,

Ralph Schwidetzky

et al.

Biogeosciences, Journal Year: 2025, Volume and Issue: 22(1), P. 103 - 115

Published: Jan. 9, 2025

Abstract. Various aerosols, including mineral dust, soot, and biological particles, can act as ice nuclei, initiating the freezing of supercooled cloud droplets. Cloud droplet significantly impacts properties and, consequently, weather climate. Some nuclei exhibit exceptionally high nucleation temperatures close to 0 °C. Ice-nucleating macromolecules (INMs) found on pollen are typically not considered among most active nuclei. Still, they be highly abundant, especially for species such Betula pendula, a widespread birch tree in boreal forest. Recent studies have shown that certain tree-derived INMs activity above −10 °C, suggesting could play more significant role atmospheric processes than previously understood. Our study reveals three distinct INM classes at −8.7, −15.7, −17.4 °C present B. pendula. Freeze drying freeze–thaw cycles noticeably alter their capability, results heat treatment, size, chemical analysis indicate correspond size-varying aggregates, with larger aggregates nucleating higher temperatures, agreement previous fungal bacterial nucleators. findings suggest pendula potentially important because prevalence temperatures.

Language: Английский

Citations

1
Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges DOI Creative Commons
Mark D. Tarn, Kirsty J. Shaw, Polly B. Foster

et al.

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

Atmospheric ice-nucleating particles (INPs) make up a vanishingly small proportion of atmospheric aerosol but are key to triggering the freezing supercooled liquid water droplets, altering lifetime and radiative properties clouds having substantial impact on weather climate. However, INPs notoriously difficult model due lack information their global sources, sinks, concentrations, activity, necessitating development new instrumentation for quantifying characterizing in rapid automated manner. Microfluidic technology has been increasingly adopted by ice nucleation research groups recent years as means performing droplet analysis INPs, enabling measurement hundreds or thousands droplets per experiment at temperatures down homogeneous water. The potential microfluidics extends far beyond this, with an entire toolbox bioanalytical separation detection techniques developed over 30 medical applications. Such methods could easily be adapted biological biogenic INP revolutionize field, example, identification quantification bacteria fungi. Combined miniaturized sampling techniques, we can envisage deployment microfluidic sample-to-answer platforms automated, user-friendly field that would enable greater understanding seasonal activity. Here, review various components such platform incorporate highlight feasibility, challenges, endeavor, from assays separations bioanalysis.

Language: Английский

Citations

0
Using Synthesized Size-Resolved Lignin Nanoparticles to Investigate the Atmospheric Ice Nucleation of Biomass Burning Organic Aerosols DOI

A Zelený,

Jingqian Chen, Paul Bieber

et al.

ACS ES&T Air, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

Citations

0
Peltigera lichen thalli produce highly potent ice-nucleating agents DOI Creative Commons
Rosemary J. Eufemio,

Galit Renzer,

Mariah Rojas

et al.

Biogeosciences, Journal Year: 2025, Volume and Issue: 22(8), P. 2087 - 2096

Published: April 29, 2025

Abstract. From extracellular freezing to cloud glaciation, the crystallization of water is ubiquitous and shapes life as we know it. Efficient biological ice nucleators (INs) are crucial for organism survival in cold environments and, when aerosolized, serve a significant source atmospheric nuclei. Several lichen species have been identified potent INs capable inducing at high subzero temperatures. Despite their importance, abundance diversity still not well understood. Here, investigate nucleation activity cyanolichen-forming genus Peltigera from across range ecosystems Arctic, northwestern United States, Central South America. We find strong IN all tested species, with temperatures above −12 °C 35 % samples initiating or −6.2 °C. The aqueous extract appear be resistant freeze–thaw cycles, suggesting that they can survive dispersal through atmosphere thereby potentially influence precipitation patterns. An axenic fungal culture termed L01-tf-B03, britannica JNU22, displays an temperature −5.6 1 mg mL−1 retains remarkably concentrations low 0.1 ng mL−1. Our analysis suggests released this fungus 1000 times more than most active bacterial Pseudomonas syringae. global distribution lichens, combination activity, emphasizes potential act powerful ice-nucleating agents atmosphere.

Language: Английский

Citations

0
Chromatic forecasting hydrogels for anti-icing applications DOI Creative Commons
Wangmeng Hou, Xiaofei Chen, Dan Wang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 26, 2025

Language: Английский

Citations

0
The presence of nanoparticles in aqueous droplets containing plant-derived biopolymers plays a role in heterogeneous ice nucleation DOI
Paul Bieber,

Ghinwa H. Darwish,

W. Russ Algar

et al.

The Journal of Chemical Physics, Journal Year: 2024, Volume and Issue: 161(9)

Published: Sept. 4, 2024

Organic matter can initiate heterogeneous ice nucleation in supercooled water droplets, thereby influencing atmospheric cloud glaciation. Predicting the ability of organic matter-containing droplets is challenging due to unknown mechanism for templating ice. Here, we observed presence nanoparticles aqueous samples known ice-nucleating biopolymers cellulose and lignin, as well newly identified xylan laminarin. Using our drop Freezing Ice Nuclei Counter (FINC), measured median temperature (T50) laminarin 2 μl be −14.2 −20.0 °C, respectively. Next, characterized these using nanoparticle tracking analysis, detected quantified with mean diameters between 132 267 nm. Xylan contained largest froze at higher temperatures. also dictated freezing a 1:1:1:1 mixture cellulose, laminarin, xylan. Filtration experiments down 300 kDa sample indicated that triggered freezing. Overall, only above 150 nm −20 °C. Furthermore, determined ice-active site densities normalized particle concentrations, surface area, mass show samples’ are similar sea spray aerosols nanometer-sized dust. The identification characterization substances expands growing list capable impacting formation thus climate.

Language: Английский

Citations

2