Genomic and functional analysis of rmp locus variants in Klebsiella pneumoniae DOI Creative Commons
Margaret M. C. Lam,

Stephen M. Salisbury,

Logan P. Treat

et al.

Genome Medicine, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 9, 2025

Abstract Background Klebsiella pneumoniae is an opportunistic pathogen and a leading cause of healthcare-associated infections in hospitals, which are frequently antimicrobial resistant (AMR). Exacerbating the public health threat posed by K. , some strains also harbour additional hypervirulence determinants typically acquired via mobile genetic elements such as well-characterised large virulence plasmid KpVP-1. The rmpADC locus considered key feature associated with upregulated capsule expression hypermucoid phenotype, can enhance contributing to serum resistance. Typically have been susceptible all antimicrobials besides ampicillin; however, recent emergence AMR concerning. Methods Here, we investigate diversity, evolution, mobilisation prevalence dataset 14,000 genomes from isolates species complex, describe RmST typing scheme for tracking variants purposes genomic surveillance. Additionally, examine functionality representatives introduced into mutant strain lacking its native locus. Results was detected 7% dataset, mostly very small number variicola quasipneumoniae . Sequence grouped five distinct lineages ( rmp1 rmp2 rmp2A rmp3 rmp4 ) that corresponded unique elements, were differentially distributed across different populations (i.e. clonal groups) All demonstrated produce enhanced production hypermucoviscosity. Conclusions These results provide overview diversity evolution prominent factor support idea screening valuable monitor spread including strains.

Language: Английский

Carbapenem-resistant hypervirulent Klebsiella pneumoniae: where is it headed in the tug-of-war between virulence and resistance? DOI Creative Commons
Meng Wang, Hui Wang

EBioMedicine, Journal Year: 2025, Volume and Issue: 114, P. 105649 - 105649

Published: March 10, 2025

Language: Английский

Citations

0
Genomic and functional analysis of rmp locus variants in Klebsiella pneumoniae DOI Creative Commons
Margaret M. C. Lam,

Stephen M. Salisbury,

Logan P. Treat

et al.

Genome Medicine, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 9, 2025

Abstract Background Klebsiella pneumoniae is an opportunistic pathogen and a leading cause of healthcare-associated infections in hospitals, which are frequently antimicrobial resistant (AMR). Exacerbating the public health threat posed by K. , some strains also harbour additional hypervirulence determinants typically acquired via mobile genetic elements such as well-characterised large virulence plasmid KpVP-1. The rmpADC locus considered key feature associated with upregulated capsule expression hypermucoid phenotype, can enhance contributing to serum resistance. Typically have been susceptible all antimicrobials besides ampicillin; however, recent emergence AMR concerning. Methods Here, we investigate diversity, evolution, mobilisation prevalence dataset 14,000 genomes from isolates species complex, describe RmST typing scheme for tracking variants purposes genomic surveillance. Additionally, examine functionality representatives introduced into mutant strain lacking its native locus. Results was detected 7% dataset, mostly very small number variicola quasipneumoniae . Sequence grouped five distinct lineages ( rmp1 rmp2 rmp2A rmp3 rmp4 ) that corresponded unique elements, were differentially distributed across different populations (i.e. clonal groups) All demonstrated produce enhanced production hypermucoviscosity. Conclusions These results provide overview diversity evolution prominent factor support idea screening valuable monitor spread including strains.

Language: Английский

Citations

0