The blast effector Pwl2 is a virulence factor that modifies the cellular localisation of host protein HIPP43 to suppress immunity DOI Creative Commons
Vincent Were, Yan Xia, Andrew J. Foster

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract The rice blast fungus Magnaporthe oryzae secretes a battery of effector proteins to facilitate host infection. Among these effectors, Pwl2 was first identified as specificity determinant for infection weeping lovegrass ( Eragrostis curvula ) and is also recognised by the barley Mla3 resistance gene. However, its biological activity not known. Here we show that PWL2 expression regulated Pmk1 MAP kinase during cell-to-cell movement M. at plasmodesmata (PD)-containing pit field sites. Consistent with regulation, provide evidence binds heavy metal-binding isoprenylated protein HIPP43, which results in displacement from plasmodesmata. Transgenic lines overexpressing either or HIPP43 exhibit attenuated immune responses increased disease susceptibility. By contrast, SNDEYWY mutant does interact fails alter PD localisation HIPP43. Targeted deletion three copies Δpwl2 showing gain-of-virulence lines, but reduction severity on susceptible plants. Taken together, our virulence factor acts suppressing immunity through perturbing plasmodesmatal deployment

Language: Английский

The blast effector Pwl2 is a virulence factor that modifies the cellular localisation of host protein HIPP43 to suppress immunity DOI Creative Commons
Vincent Were, Yan Xia, Andrew J. Foster

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract The rice blast fungus Magnaporthe oryzae secretes a battery of effector proteins to facilitate host infection. Among these effectors, Pwl2 was first identified as specificity determinant for infection weeping lovegrass ( Eragrostis curvula ) and is also recognised by the barley Mla3 resistance gene. However, its biological activity not known. Here we show that PWL2 expression regulated Pmk1 MAP kinase during cell-to-cell movement M. at plasmodesmata (PD)-containing pit field sites. Consistent with regulation, provide evidence binds heavy metal-binding isoprenylated protein HIPP43, which results in displacement from plasmodesmata. Transgenic lines overexpressing either or HIPP43 exhibit attenuated immune responses increased disease susceptibility. By contrast, SNDEYWY mutant does interact fails alter PD localisation HIPP43. Targeted deletion three copies Δpwl2 showing gain-of-virulence lines, but reduction severity on susceptible plants. Taken together, our virulence factor acts suppressing immunity through perturbing plasmodesmatal deployment

Language: Английский

Citations

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