Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
Photochemically
triggered,
transient,
and
temporally
oscillatory-modulated
transcription
machineries
are
introduced.
The
resulting
dynamic
circuits
implemented
to
guide
photochemically
oscillatory
modulation
of
thrombin
toward
temporal
control
over
fibrinogenesis.
One
system
describes
the
assembly
a
reaction
module
leading
triggered
formation
an
active
machinery
that,
in
presence
RNase
H,
guides
transient
activation
A
second
introduces
photochemical
triggering
circuit
consisting
two
coupled
machineries,
depletion
intermediate
product.
concept
is
applied
develop
leads
generation
anti-thrombin
aptamer-modified
oscillating
product
induces
rhythmic
inhibition
thrombin,
accompanied
by
cyclic
deactivation
fibrinogenesis
process.
operation
computational
kinetic
models,
allowing
predict
behaviors
under
different
auxiliary
conditions.
phototriggered
circuit-guided
examined
physiological-like
conditions
within
human
plasma
environment.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 5, 2024
Abstract
DNA
information
storage
provides
an
excellent
solution
for
metadata
due
to
its
high
density,
programmability,
and
long-term
stability.
However,
current
research
in
primarily
focuses
on
the
processes
of
storing
reading
data,
lacking
comprehensive
solutions
secure
wiping.
Herein,
we
present
a
method
random
sanitization
using
CRISPR-Cas12a
(RSDISC)
based
precise
control
thermodynamic
energy
primer-template
hybridization.
We
utilize
collateral
cleavage
(trans-activity)
single-stranded
(ssDNA)
by
achieve
selective
files
metadata.
This
enables
ssDNA
degradation
with
different
GC
content,
lengths,
secondary
structures
efficiency
up
99.9%
28,258
oligonucleotides
within
one
round.
demonstrate
that
number
erasable
could
reach
10
11.7
model
hybridization
efficiency.
Overall,
RSDISC
approach
set
foundation
encryption,
file
classification,
memory
deallocation
accurate
data
storage.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 18, 2024
Human
papillomavirus
(HPV)
screening
is
vital
for
the
early
detection
and
prevention
of
cervical
cancer.
However,
existing
methods
often
face
challenges
related
to
speed,
simplicity,
multiplexing,
especially
in
resource-limited
settings.
Here
we
developed
a
portable
SlipChip-based
multiplexed
rapid
nucleic
acid
testing
platform,
named
SMART,
designed
simultaneously
detect
HPV16
HPV18.
SMART
allows
seamless
integration
RPA
Cas12a
assays
on
SlipChip
includes
heating
membrane
regulate
on-chip
assay
temperatures.
This
operate
as
stand-alone
platform
without
additional
control
instruments.
The
also
features
an
All-in-One
imaging
mode
data
acquisition,
enhancing
its
performance.
enables
sensitive
HPV18
DNA
across
multiple
samples
just
36
min
with
limit
approximately
6
copies
per
reaction.
Testing
56
clinical
at
risk
HPV
infection
validated
SMART's
performance,
showing
97.7%
sensitivity
100%
specificity.
In
summary,
offers
system
capable
rapidly
distinguishing
between
two
most
harmful
subtypes,
showcasing
significant
potential
rapid,
various
applications.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
Photochemically
triggered,
transient,
and
temporally
oscillatory-modulated
transcription
machineries
are
introduced.
The
resulting
dynamic
circuits
implemented
to
guide
photochemically
oscillatory
modulation
of
thrombin
toward
temporal
control
over
fibrinogenesis.
One
system
describes
the
assembly
a
reaction
module
leading
triggered
formation
an
active
machinery
that,
in
presence
RNase
H,
guides
transient
activation
A
second
introduces
photochemical
triggering
circuit
consisting
two
coupled
machineries,
depletion
intermediate
product.
concept
is
applied
develop
leads
generation
anti-thrombin
aptamer-modified
oscillating
product
induces
rhythmic
inhibition
thrombin,
accompanied
by
cyclic
deactivation
fibrinogenesis
process.
operation
computational
kinetic
models,
allowing
predict
behaviors
under
different
auxiliary
conditions.
phototriggered
circuit-guided
examined
physiological-like
conditions
within
human
plasma
environment.
