Secretomes From Non‐Small Cell Lung Cancer Cells Induce Endothelial Plasticity Through a Partial Endothelial‐to‐Mesenchymal Transition

Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(5)

Published: March 1, 2025

The tumor microenvironment (TME) of non-small cell lung cancer (NSCLC) is highly heterogeneous and involved in tumorigenesis resistance to therapy. Among the cells TME, endothelial are associated with latter processes through endothelial-to-mesenchymal transition (EndMT). During EndMT, (ECs) progressively lose their phenotype favor a mesenchymal phenotype, which favors production cancer-associated fibroblasts (CAFs). Our study aimed investigate consequences exposure different secretomes on EC plasticity. Conditioned media (CM) were prepared from lines A549, H1755, H23, H1437, H1975. Proliferation migration ECs treated these CMs assessed by Cyquant Incucyte technologies, respectively. angiogenic capacity was following tubulogenesis Matrigel. Phenotypic changes detected flow cytometry. Morphological analysis actin fibers performed immunohistochemistry, while proteomic mass spectrometry used identify protein content secretomes. A change found when human umbilical vein (HUVECs) CMs. This phenotypic morphological change, an increase both stress fiber expression spontaneous migration. Furthermore, markers (α-SMA CD44) confirmed changes. However, did not modify rate double-labeled (vWF+/α-SMA+ or CD31+/CD44+). Proteomic identified potential targets EndMT therapeutic relevance. Taken together, data suggest that can induce partial EndMT.

Language: Английский

Therapeutic effects of lomerizine on vasculopathy in Fabry disease

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 2, 2025

Language: Английский

A Modified Model of PANoptosisto Identify Prognosis and Immunotherapy Response in Bladder Cancer

The International Journal of Biochemistry & Cell Biology, Journal Year: 2024, Volume and Issue: unknown, P. 106672 - 106672

Published: Oct. 1, 2024

Language: Английский