Simultaneous epitope and transcriptome measurement in single cells

Nature Methods, Journal Year: 2017, Volume and Issue: 14(9), P. 865 - 868

Published: July 31, 2017

Language: Английский

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The Human Cell Atlas

eLife, Journal Year: 2017, Volume and Issue: 6

Published: Dec. 5, 2017

The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that time is ripe to complete 150-year-old effort identify all cell types human body. Human Cell Atlas Project an international collaborative aims define terms distinctive profiles (such as gene expression profiles) and connect this information with classical cellular descriptions location morphology). An open comprehensive reference map state cells healthy tissues would propel systematic study physiological states, developmental trajectories, regulatory circuitry interactions cells, also provide framework understanding dysregulation disease. Here we describe idea, its potential utility, early proofs-of-concept, some design considerations Atlas, including commitment data, code, community.

Language: Английский

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Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Cell, Journal Year: 2016, Volume and Issue: 167(7), P. 1853 - 1866.e17

Published: Dec. 1, 2016

Language: Английский

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Mapping the Mouse Cell Atlas by Microwell-Seq

Cell, Journal Year: 2018, Volume and Issue: 172(5), P. 1091 - 1107.e17

Published: Feb. 1, 2018

Language: Английский

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Comprehensive single-cell transcriptional profiling of a multicellular organism

Science, Journal Year: 2017, Volume and Issue: 357(6352), P. 661 - 667

Published: Aug. 17, 2017

Sequencing each cell of the nematode Single-cell sequencing is challenging owing to limited biological material available in an individual and high cost across multiple cells. Cao et al. developed a two-step combinatorial barcoding method profile both single-cell single-nucleus transcriptomes without requiring physical isolation cell. The authors profiled almost 50,000 single cells from Caenorhabditis elegans larval stage were able identify recover information different, even rare, types. Science , this issue p. 661

Language: Английский

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The Technology and Biology of Single-Cell RNA Sequencing

Molecular Cell, Journal Year: 2015, Volume and Issue: 58(4), P. 610 - 620

Published: May 1, 2015

Language: Английский

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Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing

Science, Journal Year: 2015, Volume and Issue: 348(6237), P. 910 - 914

Published: May 8, 2015

Chromatin state and the single cell Identifying chromatin of any cell, which may or not have a different function represent stages relative to others collected within culture, experiment, tissue, has been challenging. Cusanovitch et al. skirted previously identified technological limitations identify regions accessible at single-cell resolution. Combinatorial cellular indexing, strategy for multiplex barcoding thousands cells per was successfully used investigate genome-wide accessibility landscape in each over 15,000 cells. Science , this issue p. 910

Language: Английский

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Assembly of functionally integrated human forebrain spheroids

Nature, Journal Year: 2017, Volume and Issue: 545(7652), P. 54 - 59

Published: April 25, 2017

Language: Английский

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Integrative single-cell analysis of transcriptional and epigenetic states in the human adult brain

Nature Biotechnology, Journal Year: 2017, Volume and Issue: 36(1), P. 70 - 80

Published: Dec. 11, 2017

Language: Английский

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Exponential scaling of single-cell RNA-seq in the past decade

Nature Protocols, Journal Year: 2018, Volume and Issue: 13(4), P. 599 - 604

Published: March 1, 2018

Language: Английский

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