Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(16)
Published: Feb. 22, 2023
Here
we
report
on
a
label-free
electrochemiluminescence
(ECL)
microscopy
using
exceptionally
low
concentrations
of
the
[Ru(bpy)3
]2+
luminophore.
This
work
addresses
central
point
minimal
concentration
ECL
luminophore
required
to
image
single
entities.
We
demonstrate
possibility
record
images
cells
and
mitochondria
at
down
nM
pM.
is
7
orders
magnitude
lower
than
classically-used
corresponds
few
hundreds
luminophores
diffusing
around
biological
Yet,
it
produces
remarkably
sharp
negative
optical
contrast
images,
as
demonstrated
by
structural
similarity
index
metric
analyses
supported
predictions
covering
time.
Finally,
show
that
reported
approach
simple,
fast,
highly
sensitive
method,
which
opens
new
avenues
for
ultrasensitive
imaging
reactivity
molecule
level.
Nature Communications,
Journal Year:
2021,
Volume and Issue:
12(1)
Published: March 5, 2021
Abstract
The
recently
introduced
minimal
photon
fluxes
(MINFLUX)
concept
pushed
the
resolution
of
fluorescence
microscopy
to
molecular
dimensions.
Initial
demonstrations
relied
on
custom
made,
specialized
microscopes,
raising
question
method’s
general
availability.
Here,
we
show
that
MINFLUX
implemented
with
a
standard
microscope
stand
can
attain
1–3
nm
in
three
dimensions,
rendering
molecule-scale
widely
applicable.
Advances,
such
as
synchronized
electro-optical
and
galvanometric
beam
steering
stabilization
locks
sample
position
sub-nanometer
precision
respect
stand,
ensure
nanometer-precise
accurate
real-time
localization
individually
activated
fluorophores.
In
our
imaging
cell-
neurobiological
samples,
~800
detected
photons
suffice
2.2
nm,
whereas
~2500
yield
precisions
<1
(standard
deviation).
We
further
demonstrate
3D
~2.4
focal
plane
~1.9
along
optic
axis.
Localizing
<20
within
~100
µs,
establish
this
spatio-temporal
single
fluorophore
tracking
apply
it
diffusion
labeled
lipids
lipid-bilayer
model
membranes.
Scientific Reports,
Journal Year:
2019,
Volume and Issue:
9(1)
Published: Aug. 27, 2019
Abstract
Mitochondria
are
highly
dynamic
organelles
that
exhibit
a
complex
inner
architecture.
They
smooth
outer
membrane
and
convoluted
forms
invaginations
called
cristae.
Imaging
cristae
in
living
cells
poses
formidable
challenge
for
super-resolution
light
microscopy.
Relying
on
cell
line
stably
expressing
the
mitochondrial
protein
COX8A
fused
to
SNAP-tag
using
STED
(
st
imulated
e
mission
d
epletion)
nanoscopy,
we
demonstrate
visualization
of
dynamics
cultivated
human
cells.
We
show
HeLa
lamellar
often
arranged
groups
separated
by
voids
generally
occupied
nucleoids.
Science,
Journal Year:
2020,
Volume and Issue:
368(6496)
Published: June 11, 2020
Receptors
moving
in
and
out
of
the
synapse
The
number
neurotransmitter
receptors
their
spatial
organization
on
postsynaptic
site
is
a
central
determinant
synaptic
efficacy.
Sophisticated
techniques
to
visualize
track
movement
single
molecules
have
provided
us
with
profound
new
insights
into
these
dynamics.
We
now
know
that
undergo
movements
different
scales.
Groc
Choquet
review
our
present
understanding
mechanisms
regulate
glutamate
receptor
localization
clustering.
Receptor
are
fundamental
basic
function
participate
many
forms
plasticity.
Science
,
this
issue
p.
eaay4631
Angewandte Chemie International Edition,
Journal Year:
2019,
Volume and Issue:
59(25), P. 9812 - 9825
Published: Nov. 14, 2019
Abstract
In
vivo
fluorescent
monitoring
of
physiological
processes
with
high‐fidelity
is
essential
in
disease
diagnosis
and
biological
research,
but
faces
extreme
challenges
due
to
aggregation‐caused
quenching
(ACQ)
short‐wavelength
fluorescence.
The
development
high‐performance
long‐wavelength
aggregation‐induced
emission
(AIE)
fluorophores
high
demand
for
precise
optical
bioimaging.
chromophore
quinoline‐malononitrile
(QM)
has
recently
emerged
as
a
new
class
AIE
building
block
that
possesses
several
notable
features,
such
red
near‐infrared
(NIR)
emission,
brightness,
marked
photostability,
good
biocompatibility.
this
minireview,
we
summarize
some
recent
advances
our
established
QM,
focusing
on
the
mechanism,
regulation
wavelength
morphology,
facile
scale‐up
fast
preparation
nanoparticles,
well
potential
biomedical
imaging
applications.
